Accompanying with the increasingly saturated genome figures,DNA chip has been widely applied.Thanks to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various ...Accompanying with the increasingly saturated genome figures,DNA chip has been widely applied.Thanks to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various research fields including biology,medicine and chemistry.This article overviews the application of DNA chip technology in animal medicine from gene expression spectrum research,pathogenic microbial detection,bacterial typing,genetic mutations and polymorphism detection,pathogenic microbial genomics research,as well as its principle and classification.展开更多
In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurat...In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurately and flexibly control the bio-molecules as they move within the channels, a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental. In this report, the current changed value of different buffer solutions, e.g., KC1, TE, and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 μm inner diameter, driven by biases of 50 or 100 mV. The results revealed that the relation- ship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation, which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.展开更多
基金Supported by National Natural Science Foundation of China(30700597)~~
文摘Accompanying with the increasingly saturated genome figures,DNA chip has been widely applied.Thanks to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various research fields including biology,medicine and chemistry.This article overviews the application of DNA chip technology in animal medicine from gene expression spectrum research,pathogenic microbial detection,bacterial typing,genetic mutations and polymorphism detection,pathogenic microbial genomics research,as well as its principle and classification.
基金supported by the Major Research Plan of the National Natural Science Foundation of China(Grant No.91123030)the Interna-tional Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant No.2011DFA12220)the National Natural Science Foundation of China(Grant No.61378083)
文摘In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurately and flexibly control the bio-molecules as they move within the channels, a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental. In this report, the current changed value of different buffer solutions, e.g., KC1, TE, and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 μm inner diameter, driven by biases of 50 or 100 mV. The results revealed that the relation- ship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation, which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.
