动物营养的“盈亏”系统

提出动物营养"盈亏"的概念,从营养"盈亏"的角度论述了我国动物营养"盈亏",重点阐述了"亏"系统的状况和发展途径,为动物营养更好的服务于畜牧业生产而进行了理论上的探讨,以期对科研和生产工作者有所启发。

关于动物营养与饲料学科课程群建设探究

动物营养与饲养学科课程群是动物学科专业的重要组成之一。有效提高动物营养与饲养学科课程群的构建,不仅能够加强各个学科之间的关联、落实教育信息分享,并且还可以最大程度推动教育形式、教育手段的革新优化,从而培育出更多的高水准、高素养的人才。本文重点探究了动物营养和饲料学科课程群构建的时代环境、价值作用、构建项目与构建策略等,意在能够为动物营养与饲养学科的教育升级带来一定帮助。

枸杞多糖的生理功能及其在动物生产中的应用

随着我国饲料“禁抗”时代的来临,天然的植物提取物逐渐成为一种新型替代抗生素的饲料添加剂。枸杞多糖是从枸杞及其副产物中提取出来的植物多糖类物质,主要由甘露糖、葡萄糖、半乳糖、阿拉伯糖、半乳糖醛酸和鼠李糖等组成,具有抗氧化、抗肿瘤以及提高机体免疫力等多种生理功能,是枸杞及其副产物的主要活性成分。本文综述了枸杞多糖的主要制备工艺、生理功能和作用机制及其在动物生产中应用的研究进展,旨在为枸杞多糖作为新型饲料添加剂在动物生产中的应用提供参考。

不同放养密度对雪峰乌骨鸡生长性能、屠宰性能、血清生化和免疫指标的影响

本试验旨在研究不同放养密度对雪峰乌骨鸡生长性能、屠宰性能、血清生化以及免疫指标的影响。选用健康的35日龄、体重一致的脱温雪峰乌骨鸡母鸡苗1620只,随机分为3个放养密度组,分别为低密度组(LSD组,60只/667 m^(2))、中密度组(MSD组,120只/667 m^(2))和高密度组(HSD组,180只/667 m^(2)),每组6个重复(林地面积500 m^(2)),LSD组每个重复45只,MSD组每个重复90只,HSD组每个重复135只。预试期7 d,正试期78 d。结果显示:1)LSD组雪峰乌骨鸡的平均日增重和料重比显著高于MSD组和HSD组(P<0.05)。2)LSD组宰前活重和屠体重均显著高于MSD组和HSD组(P<0.05)。3)LSD组血清中谷草转氨酶活性显著低于HSD组(P<0.05),LSD组和MSD组血清中碱性磷酸酶活性显著高于HSD组(P<0.05);LSD组血清中甘油三酯含量显著高于HSD组(P<0.05);HSD组血清中超氧化物歧化酶活性显著低于LSD组和MSD组(P<0.05)。4)HSD组血清中免疫球蛋白G、免疫球蛋白M、白细胞介素-2、白细胞介素-6和肿瘤坏死因子-α含量显著高于LSD组和MSD组(P<0.05),MSD组血清中免疫球蛋白A含量显著高于LSD组和HSD组(P<0.05)。由此可见,放养密度为60只/667 m^(2)时能有效提高雪峰乌骨鸡的生长性能,同时保证有较好的屠宰性能、抗氧化能力及免疫力。

Long-term modulation of the immune system by perinatal nutrition

Nutrition during perinatal period is more critical for the developme nt of the immune system than during adulthood, and the relationship between earl y nutrition and diseases in later life has been established. In humans and labor atory animals, the plasticity of metabolic function in foetuses or neonates enab les them to adapt to malnutrition for survival; however, such an adaptation, as usually evidenced by retarded growth, stunted development of lymphoid organs and impaired immunocompetence, can maintain and persist into later life even when n utrition is improved. Early nutrition may thus programme' the immune system of a nimals. Limited experimental studies have also revealed that long-term immunity against nematode parasites in sheep can be enhanced by a short-term protein su pplementation shortly after weaning, a form of 'nutritional programming', but su ch an effect appears to vanish if the nutritional status of young animals alread y meets at least the requirement for maintenance.

Acute phase reaction and acute phase proteins

A review of the systemic acute phase reaction with major cytokines involved, and the hepatic metabolic changes, negative and positive acute phase proteins (APPs) with function and associated pathology is given. It appears that APPs represent appropriate analytes for assessment of animal health. Whereas they represent non-specific markers as biological effect reactants, they can be used for assessing nutritional deficits and reactive processes, especially when positive and negative acute phase variables are combined in an index. When such acute phase index is applied to separate healthy animals from animals with some disease, much better results are obtained than with single analytes and statistically acceptable results for culling individual animals may be reached. Unfortunately at present no cheap, comprehensive and easy to use system is available for assessing various acute phase proteins in serum or blood samples at the same time. Protein microarray or fluid phase microchip technology may satisfy this need; and permit simultaneous analysis of numerous analytes in the same small volume sample and enable integration of information derived from systemic reactivity and nutrition with disease specific variables. Applying such technology may help to solve health problems in various countries not only in animal husbandry but also in human populations.

Preliminary Study on Biological Control of Cyclops of Zooplankton in Drinking Water Source

An ecological project called fish biomanipulation, other than the conventional fishery culture technique, was put forward in this paper for the excess propagation control of Cyclops. The control effects on Cyclops of four species of fish were investigated experimentally at stocking density of 30 g per cubic meter of water. The experimental results showed that the food habit of the fish had significant influence on the biological control of Cyclops. The propagation of Cyclops could be controlled effectively and also the water quality was improved simultaneously by stocking the filter-feeding fishes, such as silver carp and bighead carp. Whereas, herbivorous Ctenopharyugodon idellus and omnivorous Cyprinus carpio had no obvious biological effects on controlling the growth of Cyclops and restoring water quality. The results further proved that under condition of proper poly-culture density of silver carp and bighead carp, the number of Cyclops might be controlled at very low level and the eutrophication might be abated by removing the nutrients from water body.

Assessing benthic ecological status in stressed Liaodong Bay (China) with AMBI and M-AMBI

Rapid economic development in recent decades has resulted in environmental degradation of Liaodong Bay, North China, where eutrophication is becoming more evident because of excess nutrients inputs. To assess the benthic ecological status in Liaodong Bay, AZTI＇s Marine Biotic Index （AMBI） and multivariate-AMBI （MoAMBI） were applied using both benthic macroinvertebrate density and biomass data collected from Liaodong Bay in July 2007. This first application of AMBI and M-AMBI in Liaodong Bay showed that the nearshore areas of the bay, especially near river estuaries, were severely disturbed, with a clear gradient of disturbance decreasing seaward. Ecological status assessed from density and biomass data was quite similar. Significant relationships were also found between both indices and environmental variables in Liaodong Bay. Moreover, the spatial distributions of both AMBI and M-AMBI matched those of plotted eutrophication indices （EI） in the surface water layer, and significant linear correlations were found between both benthic indices and EI. In general, both AMBI and M-AMBI worked well on assessing the ecological status of Liaodong Bay under eutrophication stress due to excess nutrients inputs.

Effects of Replacing Fishmeal with Animal By-products Meal Supplementation in Diets on the Growth and Nutrient Utilization of Mangrove Red Snapper

A feeding trial was conducted for 75 d to evaluate the nutritive value of a mixture of animal by-products （MAB） as a possible protein source in diets for juvenile mangrove red snapper, Lutjanus argentimaculatus （mean initial body weight, 30 g）. Fish were fed one of five isonitrogenous diets （40% crude protein） replacing 0, 25% （MAB25）, 50% （MAB50）, 75% （MAB75） and 100% （MAB100） of fish meal protein with similar percentages of MAB. The MAB consisted of 25% cow liver meal, 20% leather meal, 20% meat and bone meal, 15% blood meal, 10% APC （poultry feather meal）, 8% poultry manure dried, 1.5% choline and 0.5% chromic oxide. After 75 d of feeding, fish fed with diets MAB50, MAB75 and MABI00 exhibited significantly lower growth performance than that of fish fed with control and MAB25 diets. The optimum level of MAB was estimated to be 23%. Replacement of fish meal by MAB23% showed the following performance： maximum weight gain, 510%; SGR, 2.39% and FCE, 2.83%. The MAB substitution up to 75% of fish meal protein in diets did not show differences in apparent protein digestibility （83.6% for MAB25, 79.2% for MAB50, 78.7% for MAB75） compared with control （83.4%）, whereas in MABI00 group digestibility （65.3%） was significantly lower than in other groups. The apparent phosphorus absorption of test diet groups was significantly higher （37.1% for MAB25, 28.5% for MABS0, 55.6% for MAB75 and 54.5% for MABI00） than that of control （1 1.2%）. The levels of protein and ash in the whole body, carcass and viscera increased as MAB substitution in diets increased, whereas lipids and moisture remained consistent among all treatment groups. These results showed that approximately 23% of fish meal protein could be replaced by a mixture of animal by-products for juvenile snapper growing from 30 g to 167 g in 75 d without compromising growth performance and feed efficiency.

Design and Research on Artificial Rumen Continuous Culture System

[Objective] The paper was to study the dynamic changes of forage nutrient substance fermentation in rumen, and a set of continuous culture system of artificial rumen was designed. [Method] With in vivo as control, the simulating rumen fer- mentation effect in vitro culture system was evaluated. [Result] The simulation rumen fermentation test needed adaptive phase of 2-3 d, and the fermentation state was relatively stable within 3-9 d, with good effects. The test showed certain regularity variation with index value of rumen in vivo. [Conclusion] The continuous culture sys- tem of artificial rumen could be used as the ideal model to study the rumen fermen- tation in vivo.

AVAILABILITY OF PHOSPHORUS FROM SELECTED INORGANIC PHOSPHATES TO JUVENILE ABALONE,HALIOTIS DISCUS HANNAI INO.

The availability of phosphorus to juvenile abalone, Haliotis discus hannai from primary, secondary and tertiary calcium phosphate, primary sodium or potassium phosphate separately or in combination was determined in a 120-day feeding trial. Seven semi-purified diets were formulated to contain relatively constant dietary phosphorus, ranging from 0.78%-0.82%. The concentrations of dietary phosphorus were presumed to be slightly below the requirement for abalone. A brown alga, Laminaria japonica, was used as a control diet. Abalone juveniles of similar size (average weight 1.18 g; average shell length 18.74 mm) were distributed in a single-pass, flow-through system using a completely randomized design with eight treatments and three replicates each treatment. The survival ranged from 71.1% to 81.1%, and was not significantly (P>0.05) related to dietary treatment. However, the weight gain rate (41.72%-65.02%), daily increment in shell length (36.87-55.07 μm) and muscle RNA-DNA ratio (3.44-4.69) were significantly (P<0.05) affected by dietary treatment. Soft body alkaline phosphatase activity (10.9-19.8 U/g wet tissue) and carcass levels of lipid (7.71%-9.33%) and protein (46.68%-49.35%) were significantly (P<0.05) responsive to available phosphorus of the diets. Dietary treatment had significant effect (P<0.05) on concentrations of phosphorus in the whole body (WB) and soft body (SB). Apparent digestibility coefficients (45%-97%) of phosphorus were also significantly (P<0.05) different between dietary treatments. The results indicated that among these inorganic compounds, primary calcium, potassium and sodium phosphates separately or in combination could be utilized effectively by juvenile abalone as dietary phosphorus sources (availability ranging from 87%-97%). However, secondary and tertiary calcium phosphates were found to be low in availability, the values being 45% and 77%, respectively.

Effect of Sinai San decoction on the development of non-alcoholic steatohepatitis in rats

AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into three groups randomly: control group (n = 9),model group (n = 9) and treatment group (n = 9). The rats of model group and treatment group were given small dosage of CCL4 combined with a fat-rich diet, andthose of control group were given normal diet. After four weeks of fat-rich diet feeding, the rats of treatment group were given Sinai san decoction. The serum levels of aminotransferase and lipid were measured, and the pathology of livers was observed by HE staining after the rats were sacrificed at eight weeks.RESULTS: The rats' livers presented the pathology of steatosis and inflammation with higher serum levels of ALT and AST in the model group. In the treatment group the serum ALT and AST levels decreased significantly and were close to the control group. The hepatic inflammation scores also decreased markedly, but were still higher than those of control group. And the degree of hepatocyte steatosis was similar to that of model group.CONCLUSION: Sinai san decoction may ameliorate the hepatic inflammation of rats with steatohepatitis induced by small dosage of CCL4 combined with a fat-rich diet,but does not prevent the development of hepatocyte steatosis.

White Mulberry （Morus alba） Foliage as a Feeding Supplement for Growing Calves

The aim of this study was to evaluate the feed consumption and weight gain in calves fed with a nutritional block made of white mulberry （Morus alba） for a 90-day-period, involving dry season months （March-May） in Tuxpan, Veracruz, Mexico. A total of 45 growing Zebu x Swiss calves with an average weight of 200 kg were assigned randomly in three groups of 15 animals, and the following treatments of nutritional block were offered to each group： grazing animals （To）, grazing animals plus nutritious block with white mulberry （T1）, and grazing animals plus nutritious block without white mulberry （T2）. A consumption of 0.545 kg/d from block in treatment T1 was observed, significantly improving calves growing and showing a weight gain of 0.933 kg/d. Use of white mulberry in nutritional blocks is a good alternative for growing calves, as a feeding strategy given the seasonality of pasture production in the tropics.

Use of Silage Acid Devil Fish （pterygoplichthys spp.） as Protein Supplement in Finishing Beef Cattle

Animal nutrition is one of the most important limiting factors in animal production, especially in ruminants, providing proteins being the main constraint, due to the limited availability and high cost of protein sources （oilseed meals）. Currently in the dam ＂El lnfiernillo＂ in Michoacan Mexico, has a large population of devil fish （Pterygoplichthys spp.）, which is an economic and ecological problem, because it is not consumed by humans and causes pollution to be discarded directly into the environment. For that reason the objective of this study was to evaluate the use of silage acid devil fish （SADF） in fattening beef cattle as a protein supplement. SADF is defined as a product semi-liquid or pasty mixed with formic acid, which leads to a decrease in pH to near 4.0. Used 18 young bulls （Bos taurus × Bos indicus） for 60 days with a starting weight of 278.9 ± 51.2 kg, housed in individual pens with food and water ad libitum were randomly assigned to three treatments with different levels of inclusion SADF （0%, 12% and 18%）. They were weighed to the beginning of the experiment and later every 30 days, previous fasting of 24 hours. To determine the food consumption, weigh every day the offered food and the surplus. There were no significant differences （P 〈 0.05） among treatments with different levels of inclusion of SADF with respect to daily weight gain, with values of 952 ± 324, 927 ±322 and 854 ± 307 g/day, respectively. The dry matter intake （DMI） was 8.9, 9.3 and 7.7 kg/day to 0%, 12% and 18% of SADF, respectively. In the same values for feed conversion were 9.34, 10.03 and 9.01 kg DMI/kg of weigh live, and carcass yield of 60.6%, 60.3% and 58.5%, respectively. It is concluded that fish silage acid devil is an excellent alternative in feeding beef cattle as a protein supplement.

Changes in the small-jellyfish community in recent decades in Jiaozhou Bay, China

We used long term monitoring data to evaluate changes in abundance and species dominance of small-jellyfish (collected with zooplankton net whose bell diameter was less than 5 cm) between 1991 and 2009 in the Jiaozhou Bay, China. Zooplankton samples were vertically towed with conical plankton net from near-bottom to surface, identified microscopically, and mapped in time-space using Grapher 7.0 and Surfer 8.0. Results show that the abundance of small-jellyfish throughout the bay had been increasing during 2001-2009 on average of 15.2 ind./m 3 , almost 5 times higher than that between 1991 and 2000. The occurrence of peak abundance shifted from spring to summer after 2000, and two peaks appeared in spring and summer, respectively, after 2005. Both the abundance and the frequency of blooms of small-jellyfish increased after 2000 in the bay. In addition, the biodiversity of jellyfish has increased significantly in recent years with a change in dominant species. Several new dominant species appeared after 2000, including Rathkae octopunctata in winter, Phialidium hemisphaericum in spring, summer, and autumn, Phialucium carolinae in spring, and Pleurobrachia globosa in summer and autumn, while some previous dominant species throughout the 1990s (Eirene ceylonensis, Zanclea costata, Lovenella assimilis, and Muggiaea atlantica) were no longer dominant after 2000. The abundance of small-jellyfish was positively correlated with the density of dinoflagellates, and the abundance of zooplankton. We believe that the changes in smalljellyfish abundance and species composition were the result of eutrophication, aquaculture and coastal construction activities around the bay. Concurrently, seawater warming and salinity decrease in recent decades promoted the growth and reproduction of small-jellyfish in the bay.

Studies on BN rats model to determine the potential allergenicity of proteins from genetically modified foods

AIM： To develop a Brown Norway （BN） rat model to determine the potential allergenicity of novel proteins in genetically modified food. METHODS： The allergenicity of different proteins were compared, including ovalbumin （OVA）, a potent respiratory and food allergen, bovine serum albumin （BSA）, a protein that is considered to have a lesser allergenic potential, and potato acid phosphatase （PAP）, a non-allergenic protein when administered to BN rats via different routes of exposure （intraperitoneally or by gavage）. IgG and IgE antibody responses were determined by ELISA and PEA, respectively. An immunoassay kit was used to determine the plasma histamine level. In addition, possible systemic effect of allergens was investigated by monitoring blood pressure. RESULTS： OVA provoked very vigorous protein-specific IgG and IgE responses, low grade protein-specific IgG and IgE responses were elicited by BSA, while by neither route did PAP elicit anything. In either routes of exposure, plasma histamine level in BN rats sensitized with OVA was higher than that of BSA or PAP. In addition, an oral challenge with BSA and PAP did not induce any effect on blood pressure, while a temporary drop in systolic blood pressure in few animals of each routes of exposure was found by an oral challenge with OVA. CONCLUSION： BN rat model might be a useful and predictive animal model to study the potential allergenicity of novel food proteins.

Nutritional Composition and Topoisomerase Inhibitor Activity of Ethnomedicinal Marine Mollusk Nerita albicilla

DNA topoisomerases （topo） I and II are molecular targets of several potent anticancer agents. Thus, inhibitors of these enzymes are potential candidates for anticancer development. Traditionally, Nerita albicilla had been used in Kei Island, Southern Maluku, Indonesia to treat liver disease including cancer. The paper reports on the chemical composition ofNerita albicilla and its topo I inhibitor ofhexane, ethyl acetate and methanol extracts. Topoisomerase-I inhibitor activity was determined using the method reported by TopoGEN. The proximate analysis described that Nerita albicilla dried powder contained 12.45% ± 0.05% moisture; 9.17% ± 0.03% ash; 62.05% ± 0.10% protein; 5.58% ± 0.08% fat; 6.60% ± 0.02% crude fiber and 4.15% ± 0.24% carbohydrate （by difference）. Furthermore, the protein consisted of 11 essential amino acids and six non-essential amino acids. It contained significant amount of branched-chain amino acids （BCAA） valine, leucine, isoleucine （a total of 187.8 mg g-1 protein） and lower content of aromatic amino acids phenylalanine, tyrosine and histidine （a total of 111.26 mg .g-1 protein）. The protein score was 92.2. The yield of hexane, ethyl acetate and methanol extracts ofNerita albicilla were 2.05% ± 0.05%, 1.56% ± 0.06% and 6.99% ± 0.14%, respectively. All extracts showed topoisomerase-I inhibitor activities. Minimum inhibitory concentration （MIC） of methanol extract was 2.50 ug mL-1. Chemical screening of the extracts showed that they contained steroidal and alkaloid compounds. The investigation revealed that Nerita albicilla contains active compounds that could be potential for nutraceutical or pharmaceutical development.

Food preferences by birds using bird-feeders in winter： a large-scale experiment

Background: Intentional winter bird feeding in gardens is one of the most common interactions between birds and humans. Because feeding may have both desired effects(provisioning of nutritious food for under-nourished birds) and undesired effects(favouritism of competitively superior species, transmission of disease), management of supplementary sites should be optimized from an ecological and conservation perspective. Therefore, the main aim of this study was to experimentally test winter food preferences of birds, with underlying potential influence of habitat(rural vs. urban) on realised food preferences pattern.Methods: We conducted an experimental analysis of food preferences of wintering birds by provided bird-feeders in urban and rural environments across Poland. Data were collected twice during winter 2013–2014 across Poland, in total with 80 experimental trials.Results: Sunflower seeds were the most preferred food supplement both in urban and rural habitats, significantly more exploited than any other food simultaneously available in feeders(animal fat, millet seed and dry fruits of rowanberry). However, no significant differences were recorded between urban and rural habitats in use of food.Conclusions: The degree of use of a particular type of food at bird-feeders depended on the overall use of food in a bird-feeder—consumption of each of the four types of food was significantly positively correlated with that of the others, and it was positively correlated with the number of birds observed at the feeders.

Recent Advances in Researches on Physiologically Active Substances in Holothurians

In this report, we reviewed recent literature on physiologically active substances from sea cucumbers (SCs) andtheir activities together with results obtained from our study. Preventive properties against lipid metabolism were reported inrats using a whole SC preparation with no particular constituent specified. Administration of the preparation lowered serumand hepatic cholesterol levels and improved the HDL/LDL ratio. These functions may be attributed to the stimulatory effectof the extract on the secretion of cholesterol in feces. Novel fucosylated chondroitin sulfates (FCSs) from Ludwigothureagrisea significantly induced fibroblast growth factor 2-dependent angiogenesis in human umbilical vein endothelial cells (HU-VECs). The proangiogenetic activity seemed attributable to the action of the sulfated fucose branches on the polysaccharide.SCs contain mycosporine-like amino acids (MAAs) that are capable of absorbing UV. A biogenetic precursor of MAAs wasfirst reported in SCs. The anti-proliferative effects of a branched chain fatty acid from a sea cucumber on prostate cancercells was reported with the activity of 5-lipoxygenase. Glycosphingolipid constituents in SCs have been systematically ana-lyzed over the past ten years. The results showed that the gangliosides in several SCs differed from those of mammals in thata sialic acid of SC gangliosides directly binded to glucose of cerebroside. Neuritogenic activity of the glycosphingolipids wasdemonstrated in vitro experiments and may lead to the development of therapeutic products for neurological disorders. Ourstudy also showed that sphingoid bases, the hydrolyzed products of glycosphingolipids from SCs, induced significant apoptosisin several tumor cell lines.

Does salinity change determine zooplankton variability in the saline Qarun Lake(Egypt)?

Zooplankton and 14 abiotic variables were studied during August 2011 at 10 stations in Lake Qarun, Egypt. Stations with the lowest salinity and highest nutrient concentrations and turbidity were close to the discharge of waters from the El-Bats and E1-Wadi drainage systems. A total of 15 holozooplankton species were identified. The salinity in Lake Qarun increased and fluctuated since 1901：12 g/L in 1901; 8.5 g/L in 1905; 12.0 g/L in 1922; 30.0 g/L in 1985; 38.7 g/L in 1994; 35.3 g/L in 2006, and 33.4 g/L in 2011. The mean concentration of nutrients （nitrate, nitrite and orthophosphate） gradually increased from 35, 0.16 and 0.38 μg/L, respectively, in 1953-1955 to 113, 16.4, and 30.26 μg/L in 2011. From 1999-2003 some decrease of species diversity occurred. Average total zooplankton density was 30 000 ind./m^3 in 1974-1977; 356 125 ind./m^3 in 1989; 534 000 ind./m^3 in 1994-1995; from 965 000 to 1 452 000 ind./m^3 in 2006, and 595 000 ind./m^3 in 2011. A range of long-term summer salinity variability during the last decades was very similar to a range of salinity spatial variability in summer 2011. There is no significant correlation between zooplankton abundance and salinity in spatial and long-term changes. We conclude that salinity fluctuations since at least 1955 did not directly drive the changes of composition and abundance of zooplankton in the lake. A marine community had formed in the lake, and it continues to change. One of the main drivers of this change is a regular introduction and a pressure of alien species on the existent community. Eutrophication also plays an important role. The introduction ofMnemiopsis leidyi, first reported in 2014, may lead to a start of a new stage of the biotic changes in Lake Qarun, when eutrophication and the population dynamics of this ctenophore will be main drivers of the ecosystem change.