中脑中央灰质(midbrain Central Gray,mCG)又称为大脑导水管周围灰质,是种系发生上比较古老的结构。它与Tsai氏腹侧被盖区、脚间核、Gudden氏被盖核及Bechterew氏被盖上中央核一起构成Nauta“边缘中脑区”(1)。长期以来,该结构一直为人...中脑中央灰质(midbrain Central Gray,mCG)又称为大脑导水管周围灰质,是种系发生上比较古老的结构。它与Tsai氏腹侧被盖区、脚间核、Gudden氏被盖核及Bechterew氏被盖上中央核一起构成Nauta“边缘中脑区”(1)。长期以来,该结构一直为人们所重视。特别是60年代末发现其可能参与动物镇痛作用以来,尤为人们所注目,对其进行了大量研究。有关其结构的文献十分浩繁,现就其研究进展简要综述如下:展开更多
AIM: The molecular diagnosis of microsatellite instability (MSI) in colorectal cancer (CRC) is based on the analysis of five microsatellite markers. Among them, the two mononucleotide microsatellite repeats are consid...AIM: The molecular diagnosis of microsatellite instability (MSI) in colorectal cancer (CRC) is based on the analysis of five microsatellite markers. Among them, the two mononucleotide microsatellite repeats are considered more informative for this analysis than the three dinucleotide ones. The aim of this study is to establish the most relevant markers for MSI analysis in colorectal cancers from Asian patients. METHODS: The MSI analysis of 143 CRC cases in a routine molecular diagnostic laboratory was reviewed. Analysis by fluorescence-based PCR of the five recommended microsatellites was performed, followed by data interpretation according to internationally accepted guidelines. The results were analyzed to address (1) the rate of success in the analysis of histopathological samples not specifically preparedfor molecular analysis; (2) the relative importance ofindividual markers in the diagnosis of high-MSI (H-MSI).RESULTS: MSI analysis was unsuccessful in 34 cases (24%), but for tissues archived in recent years the unsuccessful rate was 5%. We found the D2S123 marker the mostvulnerable to inadequate tissue preservation, failing to amplify in 58 instances. Approximately 30% (32/109) of the cases were H-MSI, while 7/109 (6%) were low-MSI.A detailed analysis of the H-MSI cases revealed that the dinucleotide repeats (and D5S346 in particular) were more relevant than the mononucleotide repeats in assigning the correct MSI status. CONCLUSION: The analysis of dinucleotide repeats isessential for the establishment of MSI status in Asian CRC patients.展开更多
文摘中脑中央灰质(midbrain Central Gray,mCG)又称为大脑导水管周围灰质,是种系发生上比较古老的结构。它与Tsai氏腹侧被盖区、脚间核、Gudden氏被盖核及Bechterew氏被盖上中央核一起构成Nauta“边缘中脑区”(1)。长期以来,该结构一直为人们所重视。特别是60年代末发现其可能参与动物镇痛作用以来,尤为人们所注目,对其进行了大量研究。有关其结构的文献十分浩繁,现就其研究进展简要综述如下:
基金Supported by the Health Services Development Program, Ministry of Health, Singapore (Project Reference No.: HSDP01N02). The study was carried out following the ethical approval of our Institutional Review Board (IRB Reference Code 03.104)
文摘AIM: The molecular diagnosis of microsatellite instability (MSI) in colorectal cancer (CRC) is based on the analysis of five microsatellite markers. Among them, the two mononucleotide microsatellite repeats are considered more informative for this analysis than the three dinucleotide ones. The aim of this study is to establish the most relevant markers for MSI analysis in colorectal cancers from Asian patients. METHODS: The MSI analysis of 143 CRC cases in a routine molecular diagnostic laboratory was reviewed. Analysis by fluorescence-based PCR of the five recommended microsatellites was performed, followed by data interpretation according to internationally accepted guidelines. The results were analyzed to address (1) the rate of success in the analysis of histopathological samples not specifically preparedfor molecular analysis; (2) the relative importance ofindividual markers in the diagnosis of high-MSI (H-MSI).RESULTS: MSI analysis was unsuccessful in 34 cases (24%), but for tissues archived in recent years the unsuccessful rate was 5%. We found the D2S123 marker the mostvulnerable to inadequate tissue preservation, failing to amplify in 58 instances. Approximately 30% (32/109) of the cases were H-MSI, while 7/109 (6%) were low-MSI.A detailed analysis of the H-MSI cases revealed that the dinucleotide repeats (and D5S346 in particular) were more relevant than the mononucleotide repeats in assigning the correct MSI status. CONCLUSION: The analysis of dinucleotide repeats isessential for the establishment of MSI status in Asian CRC patients.
