The interactions between human erythrocyte spectrin(SP) and Pt(II) complexes with different composition and configuration were studied by fluorescence and circular dichroism spectra. The results showed that there are ...The interactions between human erythrocyte spectrin(SP) and Pt(II) complexes with different composition and configuration were studied by fluorescence and circular dichroism spectra. The results showed that there are 4.7×10 2 binding sites of cisplatin(CDDP) in a spectrin tetramer(SPT). Among them, about 70 sites with apparent binding constant K 1】3.47×10 6 were of highest affinity, 1.8×10 2 sites with K 2 = 3.47×10 6 were of high affinity, and other 2.2×10 2 sites with K 3 = 8.77×10 5 were of low affinity. The conformation change of spectrin, depending on the concentration of Pt(II) complex and molar ratio(R) of Pt(II) complex to spectrin, was induced by the binding of Pt(II) complexes. It indicated that the interaction of both CDDP and cis diaquodiamine platinum(DADP) with SP followed a two step first order kinetic process in the first stage (1 h), and the kinetic constants were determined. In the second stage, the induced conformation change, polymerization and depolymerization of SP were probably involved. It was noticed that in the reaction of SP and Pt(II) complexes with 1,2 cyclohexanediammine isomers as chiral carrier ligand, stereo matching played a more important role than the affinity of Pt(II) to thiol groups of SP.展开更多
The morphology of tissue structures composing the pyloric orifice is thought to play a role in effectively mixing aqueous gastric effluent with duodenal secretions. To understand the physical mechanisms leading to eff...The morphology of tissue structures composing the pyloric orifice is thought to play a role in effectively mixing aqueous gastric effluent with duodenal secretions. To understand the physical mechanisms leading to efficient digestion requires computational models that allow for analyses of the contributions of individual structural components. Thus, we have simulated 2-D channel flows through representative models of the duodenum with moving boundary capabilities in order to quantitatively assess the importance of notable features. A well-tested flow solver was used to computationally isolate and compare geometric and kinematic parameters that lead to various characteristics of fluid motion at the antroduodenal junction. Scalar variance measurement was incorporated to quantify the mixing effectiveness of each component. It was found that the asymmetric geometry of the pyloric orifice in concert with intermittent gastric outflow and luminal constriction is likely to enhance homogenization of gastric effluent with duodenal secretions.展开更多
Whilst methods exist to indirectly measure the effects of increased flow or gastro-oesophageal refluxing, they cannot quantitatively measure the amount of acid travelling back up into the oesophagus during reflux, nor...Whilst methods exist to indirectly measure the effects of increased flow or gastro-oesophageal refluxing, they cannot quantitatively measure the amount of acid travelling back up into the oesophagus during reflux, nor can they indicate the flow rate through the oesophagogastric junction (OGJ). Since OGJ dysfunction affects flow it seems most appropriate to describe the geometry of the OGJ and its effect on the flow. A device known as the functional lumen imaging probe (FLIP) has been shown to reliably measure the geometry of and pressure changes in the OGJ. FLIP cannot directly measure flow but the data gathered from the probe can be used to model flow through the junction by using computational flow dynamics (CFD). CFD uses a set of equations known as the Navier-Stokes equations to predict flow patterns and is a technique widely used in engineering. These equations are complex and require appropriate assumptions to provide simplifications before useful data can be obtained. With the assumption that the cross-sectional areas obtained via FLIP are circular, the radii of these circles can be obtained. A cubic interpolation scheme can then be applied to give a high-resolution geometry for the OGJ. In the case of modelling a reflux scenario, it can be seen that at the narrowest section a jet of fluid squirts into the oesophagus at a higher velocity than the fluid surrounding it. This jet has a maximum velocity of almost 2 ms-1 that occurs where the OGJ is at its narrowest. This simple prediction of acid ‘squirting’ into the oesophagusillustrates how the use of numerical methods can be used to develop a better understanding of the OGJ. This initial work using CFD shows some considerable promise for the future.展开更多
As responses to metropolitan suburbanization and rural urbanization,the formation and evolution of urban fringes should be understood against the background of overall economic development and spatial reconstruction o...As responses to metropolitan suburbanization and rural urbanization,the formation and evolution of urban fringes should be understood against the background of overall economic development and spatial reconstruction of entire metropolises.At the same time,however,endogenous interactions between industrial structure and spatial patterns of non-agricultural activities are also worthy of scholarly attention.Since the 1980s,studies on urban fringes in China have been restricted by the lack of micro-level data.This paper investigates the spatial expansion and structural evolution of the urban fringe by taking the case of Beijing and uses systematic firm-level data in 1996 and 2001 from the National Census of Basic Units.The diversity of distribution patterns across industrial sectors brings about two interrelated results.On the one hand,structural adjustment of non-agricultural industries promotes the expansion and spatial evolution of the urban fringe.On the other hand,the stability and dynamics of industrial structure coexist in the moving urban fringe.This study also reveals that the outward-moving urban fringe is the optimal location for manufacturing,especially heavy manufacturing,as well as traditional producer and consumer services.However,industries with spatial stickiness such as tourism and sports have not moved with the fringe.Most advanced services remain concentrated in the city center.The authors argue that it is essential for understanding and managing urban fringes to take into account spatial evolution and industrial structural adjustment together with their interaction with each other.展开更多
文摘The interactions between human erythrocyte spectrin(SP) and Pt(II) complexes with different composition and configuration were studied by fluorescence and circular dichroism spectra. The results showed that there are 4.7×10 2 binding sites of cisplatin(CDDP) in a spectrin tetramer(SPT). Among them, about 70 sites with apparent binding constant K 1】3.47×10 6 were of highest affinity, 1.8×10 2 sites with K 2 = 3.47×10 6 were of high affinity, and other 2.2×10 2 sites with K 3 = 8.77×10 5 were of low affinity. The conformation change of spectrin, depending on the concentration of Pt(II) complex and molar ratio(R) of Pt(II) complex to spectrin, was induced by the binding of Pt(II) complexes. It indicated that the interaction of both CDDP and cis diaquodiamine platinum(DADP) with SP followed a two step first order kinetic process in the first stage (1 h), and the kinetic constants were determined. In the second stage, the induced conformation change, polymerization and depolymerization of SP were probably involved. It was noticed that in the reaction of SP and Pt(II) complexes with 1,2 cyclohexanediammine isomers as chiral carrier ligand, stereo matching played a more important role than the affinity of Pt(II) to thiol groups of SP.
文摘The morphology of tissue structures composing the pyloric orifice is thought to play a role in effectively mixing aqueous gastric effluent with duodenal secretions. To understand the physical mechanisms leading to efficient digestion requires computational models that allow for analyses of the contributions of individual structural components. Thus, we have simulated 2-D channel flows through representative models of the duodenum with moving boundary capabilities in order to quantitatively assess the importance of notable features. A well-tested flow solver was used to computationally isolate and compare geometric and kinematic parameters that lead to various characteristics of fluid motion at the antroduodenal junction. Scalar variance measurement was incorporated to quantify the mixing effectiveness of each component. It was found that the asymmetric geometry of the pyloric orifice in concert with intermittent gastric outflow and luminal constriction is likely to enhance homogenization of gastric effluent with duodenal secretions.
基金Supported by a Science Foundation Ireland through the ETS Walton Visiting Professor Programme a grant from the Higher Education Authority in Ireland
文摘Whilst methods exist to indirectly measure the effects of increased flow or gastro-oesophageal refluxing, they cannot quantitatively measure the amount of acid travelling back up into the oesophagus during reflux, nor can they indicate the flow rate through the oesophagogastric junction (OGJ). Since OGJ dysfunction affects flow it seems most appropriate to describe the geometry of the OGJ and its effect on the flow. A device known as the functional lumen imaging probe (FLIP) has been shown to reliably measure the geometry of and pressure changes in the OGJ. FLIP cannot directly measure flow but the data gathered from the probe can be used to model flow through the junction by using computational flow dynamics (CFD). CFD uses a set of equations known as the Navier-Stokes equations to predict flow patterns and is a technique widely used in engineering. These equations are complex and require appropriate assumptions to provide simplifications before useful data can be obtained. With the assumption that the cross-sectional areas obtained via FLIP are circular, the radii of these circles can be obtained. A cubic interpolation scheme can then be applied to give a high-resolution geometry for the OGJ. In the case of modelling a reflux scenario, it can be seen that at the narrowest section a jet of fluid squirts into the oesophagus at a higher velocity than the fluid surrounding it. This jet has a maximum velocity of almost 2 ms-1 that occurs where the OGJ is at its narrowest. This simple prediction of acid ‘squirting’ into the oesophagusillustrates how the use of numerical methods can be used to develop a better understanding of the OGJ. This initial work using CFD shows some considerable promise for the future.
基金Under the auspices of National Natural Science Foundation of China(No.40830746,40871075)
文摘As responses to metropolitan suburbanization and rural urbanization,the formation and evolution of urban fringes should be understood against the background of overall economic development and spatial reconstruction of entire metropolises.At the same time,however,endogenous interactions between industrial structure and spatial patterns of non-agricultural activities are also worthy of scholarly attention.Since the 1980s,studies on urban fringes in China have been restricted by the lack of micro-level data.This paper investigates the spatial expansion and structural evolution of the urban fringe by taking the case of Beijing and uses systematic firm-level data in 1996 and 2001 from the National Census of Basic Units.The diversity of distribution patterns across industrial sectors brings about two interrelated results.On the one hand,structural adjustment of non-agricultural industries promotes the expansion and spatial evolution of the urban fringe.On the other hand,the stability and dynamics of industrial structure coexist in the moving urban fringe.This study also reveals that the outward-moving urban fringe is the optimal location for manufacturing,especially heavy manufacturing,as well as traditional producer and consumer services.However,industries with spatial stickiness such as tourism and sports have not moved with the fringe.Most advanced services remain concentrated in the city center.The authors argue that it is essential for understanding and managing urban fringes to take into account spatial evolution and industrial structural adjustment together with their interaction with each other.
