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治疗性低温对大鼠心肌细胞收缩功能的保护作用
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作者 周晓辉 蔡玥 +4 位作者 郭珊珊 吴清华 王淑梅 王娜 张博 《川北医学院学报》 CAS 2023年第11期1475-1479,共5页
目的:探讨温度对大鼠心肌细胞收缩功能的影响,并寻找可用于心肌保护的最佳温度。方法:用酶解法急性分离大鼠左室心肌细胞并复钙,采用可视化单细胞动缘探测系统检测并比较逐步升温(25~37℃)和恒温孵育(25、30、35℃)对大鼠心肌细胞收缩... 目的:探讨温度对大鼠心肌细胞收缩功能的影响,并寻找可用于心肌保护的最佳温度。方法:用酶解法急性分离大鼠左室心肌细胞并复钙,采用可视化单细胞动缘探测系统检测并比较逐步升温(25~37℃)和恒温孵育(25、30、35℃)对大鼠心肌细胞收缩功能的影响,用收缩幅度的相对变化作为评价指标,明确能使心肌细胞产生最大且最稳定收缩的温度。结果:27~37℃逐步升温过程中,大鼠心肌细胞接受持续电刺激后稳定收缩的比例随温度的升高而下降。27~30℃逐步温度升高过程中,大鼠心肌细胞收缩幅度与25℃相比逐渐增大(P<0.05);31~37℃时,随着温度继续上升,心肌细胞出现不规律收缩、形态改变,甚至挛缩死亡,且收缩幅度与25℃比较,差异无统计学意义(P>0.05)。25℃、30℃、35℃固定恒温孵育后的心肌细胞收缩幅度两两比较,差异均有统计学意义(P<0.05),其中30℃孵育的细胞收缩幅度最大且稳定(P<0.05)。结论:温度变化影响大鼠心肌细胞的收缩功能,30℃时细胞收缩力强且稳定。 展开更多
关键词 心肌细胞 温度 动缘检测 收缩
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大鼠心肌细胞分离方法的改进 被引量:13
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作者 石晓路 柳絮 +5 位作者 郭会彩 张丽男 勾向博 熊晨 苏倩 王永利 《中国药理学通报》 CAS CSCD 北大核心 2010年第5期687-690,共4页
目的介绍一种易于判断酶解终点的分离大鼠心肌细胞的方法。方法成年SD大鼠麻醉后开胸取出心脏,分别采用传统的Langendorff灌流装置和本实验室改进的Langen-dorff灌流装置,于恒温37℃灌流消化液(含0.6%胶原酶B,0.6%BSA,30μmol.L-1Ca2+... 目的介绍一种易于判断酶解终点的分离大鼠心肌细胞的方法。方法成年SD大鼠麻醉后开胸取出心脏,分别采用传统的Langendorff灌流装置和本实验室改进的Langen-dorff灌流装置,于恒温37℃灌流消化液(含0.6%胶原酶B,0.6%BSA,30μmol.L-1Ca2+的台氏液)分离细胞,并采用IonOptix单细胞动缘检测系统检测细胞功能。结果传统的Langendorff灌流装置分离细胞,酶解时间约13~16min,但此法终点难以判断,每次分离的细胞质量差别较大,且不耐钙,收缩的稳定性较差。而以改进的Langendorff灌流装置分离细胞易于确定消化终点,细胞成活率大于80%,在含1.8mmol.L-1Ca2+的台氏液中存活率也高达50%。给予电压15V、频率1Hz、波宽4ms的持续电刺激时,其在1000s内收缩舒张功能稳定。结论经改造的Langendorff灌流装置分离的心肌细胞成活率高,可控性强,结果稳定,能够更好地用于检测细胞收缩舒张功能的实验。 展开更多
关键词 LANGENDORFF 心肌细胞 细胞分离 终点判断 动缘检测 收缩
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PHOTO-ELECTRIO MEASURING SYSTEM FOR TOOL EDGE DETECTION BY THE TEOHNOLOGY OF COMPUTER DYNAMIO DISPLAY MOVING IMAGE
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作者 孙旭东 林玉池 张国雄 《Transactions of Tianjin University》 EI CAS 1999年第1期47-51,共5页
This paper describes a technology of dynamic display moving image by computer monitor,which is initially used in the design of tool detection system. The paper presents the hardware and software principie and edge det... This paper describes a technology of dynamic display moving image by computer monitor,which is initially used in the design of tool detection system. The paper presents the hardware and software principie and edge detection process. The way of marking edge point coordinates and stability of moving image also is analyzed. The method reforms the conventional design of the 2-D vision detection system. Moreover,it facilitates the design of the systematic mechanical construction,is convenient to compile instrument systemsoftware,and realizes to detect and track display image simultaneously. By the work,the tool detection system is improved to practical application. 展开更多
关键词 edge detection dynamic display tool detection instrument
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Automatic Damage Detection of Engineering Ceramics Ground Surface Based on Texture Analysis 被引量:2
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作者 林滨 陈善功 +1 位作者 韩雪松 梁小虎 《Transactions of Tianjin University》 EI CAS 2013年第4期267-271,共5页
Ground textures seriously interfere with the exact identification of grinding damage. The common nondestructive testing techniques for engineering ceramics are limited by their difficulty and cost. Therefore, this pap... Ground textures seriously interfere with the exact identification of grinding damage. The common nondestructive testing techniques for engineering ceramics are limited by their difficulty and cost. Therefore, this paper proposes a global image reconstruction scheme in ground texture surface using Fourier transform (FT). The lines associated with high-energy frequency components in the spectrum that represent ground texture information can be detected by Hough transform (HT), and the corresponding high-energy frequency components are set to zero. Then the spectrum image is back-transformed into the spatial domain image with inverse Fourier transform (IFT). In the reconstructed image, the main ground texture information has been removed, whereas the surface defects information is preserved. Finally, Canny edge detection is used to extract damage image in the reconstructed image. The experimental results of damage detection for the ground texture surfaces of engineering ceramics have shown that the proposed method is effective. 展开更多
关键词 engineering ceramics damage detection ground texture Fourier transform
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Study on calculation for the position and scope of tropical cyclone
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作者 刘永禄 邵利民 郎丰旺 《Marine Science Bulletin》 CAS 2013年第2期37-45,共9页
The scale, shape and position are three main factors to forecast tropical cyclone. The aim of the paper is to recognize tropical cyclone (TC) in the satellite cloud pictures according to the scale, shape and positio... The scale, shape and position are three main factors to forecast tropical cyclone. The aim of the paper is to recognize tropical cyclone (TC) in the satellite cloud pictures according to the scale, shape and position of clouds. The study includes Canny edge detection, contour extraction and other techniques. The solutions are also established. The experiments show that the method can recognize the TC in the satellite pictures. The study is beneficial for TC track. 展开更多
关键词 tropical cyclone (TC) Canny edge detection contour extraction automation recognition
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Using Sobel Operator for Automatic Edge Detection in Medical Images 被引量:3
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作者 A.V. Doronicheva A. A. Socolov S. Z. Savin 《Journal of Mathematics and System Science》 2014年第4期257-260,共4页
The problems of installation and integration of complex suite of software for processing medical images. Based analysis of the situation is realized in an easier integration of an automated system using the latest inf... The problems of installation and integration of complex suite of software for processing medical images. Based analysis of the situation is realized in an easier integration of an automated system using the latest information technologies using the web - environment for analysis and segmentation of DICOM - images. 展开更多
关键词 SEGMENTATION medical image the algorithm contouring the Ring Road the quality of medical images
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Iris Localization Algorithm Based on Improved Generalized Symmetry Transform
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作者 左坤隆 刘文耀 +1 位作者 朱昊 王晓东 《Transactions of Tianjin University》 EI CAS 2004年第4期259-264,共6页
Accuracy and fastness of iris localization are very important in automatic iris recognition. A new fast iris localization algorithm based on improved generalized symmetry transform (GST) was proposed by utilizing (iri... Accuracy and fastness of iris localization are very important in automatic iris recognition. A new fast iris localization algorithm based on improved generalized symmetry transform (GST) was proposed by utilizing (iris) symmetry. GST was improved in three aspects:1) A new distance weight function is defined. The new weight function, which is effective in iris localization, utilized the characteristic of irises that the iris is a circular object and it has one inner boundary and one outer boundary. 2) Each calculation of the symmetry measurement of a pair of symmetry points was performed by taking one point of a pair as the starting point of the transformation. This is the most important reason for fast iris localization,due to which, repetitious computation was largely excluded. 3) A new phase weight function was proposed to adjust GST to locate circle target much better because the inner part of iris is darker than the outer part. The edge map of iris image was acquired and GST was only implemented on the edge point, which decreased computation without loss of accuracy. The modification of distance weight function and phase weight function leads to the accuracy of localization, and other ideas speed up the localization. Experiments show that the average speed of new algorithm is about 7.0—8.5 times as high as traditional ones including integro-differential operator and Hough transform method. 展开更多
关键词 iris recognition edge detection MORPHOLOGY generalized symmetry transform (GST)
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A New Fast Edge Detection Algorithm Based on NAM-structured Plane Decomposition
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作者 WU Xueli CHEN Chuanbo SAREM Mudar HUANG Wei 《Geo-Spatial Information Science》 2009年第4期281-288,共8页
The nonsymmetry and antipacking pattern representation model (NAM), inspired by the concept of the packing problem, uses a set of subpatterns to represent an original pattern. The NAM is a promising method for image... The nonsymmetry and antipacking pattern representation model (NAM), inspired by the concept of the packing problem, uses a set of subpatterns to represent an original pattern. The NAM is a promising method for image representation because of its ability to focus on the interesting subsets of an image. In this paper, we develop a new method for gray-scale image representation based on NAM, called NAM-structured plane decomposition (NAMPD), in which each subpattern is associated with a rectangular region in the image. The luminance function of pixels in this region is approximated by an oblique plane model. Then, we propose a new and fast edge detection algorithm based on NAMPD. The theoretical analyses and experimental results presented in this paper show that the edge detection algorithm using NAMPD performs faster than the classical ones because it permits the execution of operations on subpatterns instead of pixels. 展开更多
关键词 nonsymmetry and antipacking pattern representation model (NAM) packing problem image processing edge detection
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