Hierarchical clustering analysis have been done on 48 strains of the representative insects inclusion body viruses (26 strains of nuclear polyhedrosls viruses, 13 strains of granulosis viruses and 9 strains of cytopla...Hierarchical clustering analysis have been done on 48 strains of the representative insects inclusion body viruses (26 strains of nuclear polyhedrosls viruses, 13 strains of granulosis viruses and 9 strains of cytoplasmic polyhedrosis viruses) by capillary gas chromatography, using the eight strategies of hierarchical clustering of Euclidean distance coefficient. A comparison between the dendrograms obtained by these strategies has been made. The results showed that there are definite discriminations between baculovirus group (genus) and cytoplasmic polyhedrosis virus group (genus). The relationships among the genera, groups, subgroups and strains could clearly be unfolded. The similarities and differences among strains also be distinguished. The average method, flexible method （β=0.00）, and group average method in eight strategies of hierarchical clustering had the advantage of other strategies.展开更多
本试验根据Gen Bank中I群禽腺病毒(FAV-Ⅰ)代表株AAU46933(CELO株)的Hexon基因序列设计一对通用引物,摸索了合适的扩增条件,建立了SYBR Green I荧光定量PCR方法,并对9株鸡包涵体肝炎毒株的病毒滴度进行了测定,并与OD260法、鸡胚病变法...本试验根据Gen Bank中I群禽腺病毒(FAV-Ⅰ)代表株AAU46933(CELO株)的Hexon基因序列设计一对通用引物,摸索了合适的扩增条件,建立了SYBR Green I荧光定量PCR方法,并对9株鸡包涵体肝炎毒株的病毒滴度进行了测定,并与OD260法、鸡胚病变法以及细胞病变法测定的结果进行比较。试验结果表明,本方法的检测下限为0.98×102拷贝/μL。以细胞病变法为标准测定方法,经统计学分析发现,鸡胚病变法与细胞病变法之间无统计学差异(P=0.591);荧光定量PCR法与细胞病变法测得结果之间存在线性相关性(相关系数r=0.965,P<0.05);OD260法与细胞病变法测得的结果间无相关性(P>0.05)。以上结果说明,荧光定量PCR法测定结果能够间接反映病毒感染力,与传统方法相比,该方法操作简单、耗时短、成本低,具有较高的应用价值。展开更多
文摘Hierarchical clustering analysis have been done on 48 strains of the representative insects inclusion body viruses (26 strains of nuclear polyhedrosls viruses, 13 strains of granulosis viruses and 9 strains of cytoplasmic polyhedrosis viruses) by capillary gas chromatography, using the eight strategies of hierarchical clustering of Euclidean distance coefficient. A comparison between the dendrograms obtained by these strategies has been made. The results showed that there are definite discriminations between baculovirus group (genus) and cytoplasmic polyhedrosis virus group (genus). The relationships among the genera, groups, subgroups and strains could clearly be unfolded. The similarities and differences among strains also be distinguished. The average method, flexible method （β=0.00）, and group average method in eight strategies of hierarchical clustering had the advantage of other strategies.
文摘本试验根据Gen Bank中I群禽腺病毒(FAV-Ⅰ)代表株AAU46933(CELO株)的Hexon基因序列设计一对通用引物,摸索了合适的扩增条件,建立了SYBR Green I荧光定量PCR方法,并对9株鸡包涵体肝炎毒株的病毒滴度进行了测定,并与OD260法、鸡胚病变法以及细胞病变法测定的结果进行比较。试验结果表明,本方法的检测下限为0.98×102拷贝/μL。以细胞病变法为标准测定方法,经统计学分析发现,鸡胚病变法与细胞病变法之间无统计学差异(P=0.591);荧光定量PCR法与细胞病变法测得结果之间存在线性相关性(相关系数r=0.965,P<0.05);OD260法与细胞病变法测得的结果间无相关性(P>0.05)。以上结果说明,荧光定量PCR法测定结果能够间接反映病毒感染力,与传统方法相比,该方法操作简单、耗时短、成本低,具有较高的应用价值。
