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地被菊匍匐性的遗传分析与RAPD标记研究 被引量:14
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作者 赵静媛 陈发棣 +1 位作者 滕年军 陈素梅 《中国农业科学》 CAS CSCD 北大核心 2009年第2期734-741,共8页
【目的】探讨地被菊株型匍匐性的遗传控制,并寻找与匍匐性连锁的RAPD标记,为匍匐型地被菊新品种选育、分子标记辅助育种和匍匐性相关基因的克隆奠定基础。【方法】以盆栽小菊品种‘早意大利红’为母本,地被菊品种‘03(6)-12’为父本构... 【目的】探讨地被菊株型匍匐性的遗传控制,并寻找与匍匐性连锁的RAPD标记,为匍匐型地被菊新品种选育、分子标记辅助育种和匍匐性相关基因的克隆奠定基础。【方法】以盆栽小菊品种‘早意大利红’为母本,地被菊品种‘03(6)-12’为父本构建F1群体,从F1中选取株型表现为匍匐、直立和中间型的单株各1株,分别自交,构建F2群体,并以选取的3个类型F1单株为母本与‘03(6)-12’回交,构建BC1群体。以主枝分枝角度作为株型划分依据,统计每个群体中不同株型分离比,推测地被菊株型控制相关基因特点。此外,以F1分离群体为试材,采用集团分离分析法(bulkedsegregantanalysis,BSA)构建株型匍匐/直立基因池,利用200个RAPD随机引物筛选与地被菊匍匐基因相连锁的分子标记。【结果】通过遗传分析和χ2检验表明,地被菊匍匐/直立特性由1对不完全显性主效基因控制,同时存在微效修饰基因。筛选出1个与地被菊匍匐性状控制基因相连锁的分子标记A-10555。经过重复性验证和群体单株验证,该标记与地被菊株型匍匐/直立位点遗传距离为7.96cM。【结论】以主枝分枝角度作为株型的划分标准是可行的,通过F1、F2和BC1分离群体能对地被菊株型控制位点进行有效分析。获得的分子标记与株型匍匐/直立位点的遗传距离小,能用于辅助育种。 展开更多
关键词 地被菊 株型匍匐性 遗传分析 集团分离分析法(BSA) RAPD标记
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与地被菊株型匍匐性连锁RAPD标记的SCAR转化 被引量:9
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作者 赵静媛 陈素梅 陈发棣 《林业科学》 EI CAS CSCD 北大核心 2009年第9期147-150,共4页
In this study,PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum(Dendranthema grandiflorum) was recovered,and subsequently subcloned and ... In this study,PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum(Dendranthema grandiflorum) was recovered,and subsequently subcloned and sequenced. A pair of 18-base specific primers was designed based on the obtained sequence.The validity of this SCAR marker was verified by screening parents and 152 individuals of the F1 progeny,and the consistence in the locus of the special band and the number of recombinants was observed between SCAR analysis and RAPD analysis. The size of this SCAR marker was 555 bp,which was designated as SCA10555. The results demonstrated that the RAPD marker was converted to SCAR marker successfully. This study provides bases for new cultivars developing,molecular markers assisted breeding and creeping habit related genes cloning of the ground-cover chrysanthemum. 展开更多
关键词 地被菊 株型匍匐性 SCAR标记
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西南地区扁穗牛鞭草野生资源匍匐性研究 被引量:6
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作者 刘金平 张新全 陈永霞 《安徽农业科学》 CAS 北大核心 2005年第5期796-798,共3页
以基株为单位,匍匐茎长与自然高度比为形态指数,对西南地区60份扁穗牛鞭草[(HemarthriaCompressa(L.F.)R.Br.)]野生材料进行匍匐性研究。结果表明:西南地区牛鞭草形态差异很大,具有显著的形态多样性;自然高度与匍匐茎长变异系数分别为42... 以基株为单位,匍匐茎长与自然高度比为形态指数,对西南地区60份扁穗牛鞭草[(HemarthriaCompressa(L.F.)R.Br.)]野生材料进行匍匐性研究。结果表明:西南地区牛鞭草形态差异很大,具有显著的形态多样性;自然高度与匍匐茎长变异系数分别为42.62%和18.87%;形态指数变异大,最大值是H049的12.079,最小值是H002的1.133,两者相差90%,形态指数的变异系数高达55.81%。匍匐性与材料原生地海拔高度关系不大,相关系数为-0.03592(F=0.8004>0.5)。匍匐性与自然高度的相关系数为-0.71945,存在明显的负相关(F<0.0001),具有极高的置信度。对匍匐性聚类分析,材料分为7类,可依据匍匐性类型,有针对性地对资源进行研究、驯化和选育。 展开更多
关键词 西南地区 扁穗牛鞭草 野生资源 匍匐性 聚类分析 牧草
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匍匐性矮牵牛的扦插繁殖
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作者 郑雪东 《新疆林业》 2004年第5期36-36,共1页
匍匐性矮牵牛属于长日照植物,从播种到开花约需春季90~110天左右,夏季70~90天左右,而本人在多次试验的情况下,从扦插到开花冬季需60~80天,且长势良好,匍匐性能亦良好,具体作法如下:
关键词 匍匐性矮牵牛 扦插繁殖 肥水管理 插穗
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我国狗牙根种质资源匍匐性的研究 被引量:17
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作者 刘建秀 郭爱桂 郭海林 《中国草地》 CSCD 2002年第2期36-38,共3页
利用草坪草匍匐性的量化指标—形态指数对我国狗牙根种质资源匍匐性进行了研究和评价。结果表明 ,我国狗牙根种质资源形态指数变异系数为 4 8.5 % ,种源间形态指数差异极显著 ,并且随纬度增大呈显著减小趋势 (y =0 .70 6 - 0 .0 2 4x) ... 利用草坪草匍匐性的量化指标—形态指数对我国狗牙根种质资源匍匐性进行了研究和评价。结果表明 ,我国狗牙根种质资源形态指数变异系数为 4 8.5 % ,种源间形态指数差异极显著 ,并且随纬度增大呈显著减小趋势 (y =0 .70 6 - 0 .0 2 4x) ,而随经度增大呈增大趋势 (y =0 .371+0 .0 0 2 4x)。多重比较结果表明 ,C191、C2 97及C2 19的形态指数显著高于Tifway。 展开更多
关键词 狗牙根 种质资源 形态指数 匍匐性 中国 牧草
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大麦新矮源华矮11的耐寒性研究
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作者 韩永璞 雷成军 《甘肃农业科技》 2021年第4期56-60,共5页
以母本华矮11、父本华大麦6号和华矮11/华大麦6号122个株系的DH群体为研究对象,通过调查耐寒相关形态指标来选择耐寒性强的大麦株系。结果表明,大麦苗期幼苗匍匐性越好,其耐寒性也越强,更能安全度过低温寒害;返青期前,SPAD值与其耐寒程... 以母本华矮11、父本华大麦6号和华矮11/华大麦6号122个株系的DH群体为研究对象,通过调查耐寒相关形态指标来选择耐寒性强的大麦株系。结果表明,大麦苗期幼苗匍匐性越好,其耐寒性也越强,更能安全度过低温寒害;返青期前,SPAD值与其耐寒程度呈显著正相关;成熟期空秕率、产量与SPAD值相关性不显著,说明该株系的耐寒性遗传受多种数量基因的影响。 展开更多
关键词 大麦 新矮源 华矮11 耐寒 匍匐性 SPAD值
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Architectural Plasticity in Response to Soil Moisture in the Stoloniferous Herb, Duchesnea indica 被引量:17
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作者 罗学刚 董鸣 《Acta Botanica Sinica》 CSCD 2002年第1期97-100,共4页
Architectural plasticity of clonal plants may enhance exploitation of soil moisture heterogeneity by the plants. The plasticity of clonal architecture in response to soil moisture in the stoloniferous herb, Duchesne... Architectural plasticity of clonal plants may enhance exploitation of soil moisture heterogeneity by the plants. The plasticity of clonal architecture in response to soil moisture in the stoloniferous herb, Duchesnea indica Focke, was investigated in an experiment with different soil moisture contents as treatments, i.e. 40%, 60%, 80%, 100% of the maximum moisture content of soil (MMCS). As soil moisture content increased, the spacer length, ramet density, branching intensity and branching angle of D. indica plants changed by quadratic curve. And the optimum habitat for the plants was at 80% of the MMCS. This architectural plasticity in D. indica was simulated through the Dynamic Logistic Model. The imitative effect was statistically satisfactory. Its architectural plasticity observed here may allow the species to show foraging behavior in its habitat where soil moisture is patchily distributed. 展开更多
关键词 Duchesnea indica clonal plant soil moisture clonal architecture PLASTICITY
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Intraclonal Resource Sharing and Functional Specialization of Ramets in Response to Resource Heterogeneity in Three Stoloniferous Herbs 被引量:8
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作者 于飞海 董鸣 张称意 《Acta Botanica Sinica》 CSCD 2002年第4期468-473,共6页
Environments with reciprocal patchiness of resources, in which the availability of two resources such as light and soil nutrients are patchily distributed in horizontal space and negatively correlated in each patch, a... Environments with reciprocal patchiness of resources, in which the availability of two resources such as light and soil nutrients are patchily distributed in horizontal space and negatively correlated in each patch, are common in many ecosystems. The strategies by which clonal plants adapt to this type of heterogeneous environment were examined in three stoloniferous herbs,Potentilla reptans L. var. sericophylla Franch., P. anserina L. and Halerpestes ruthenica (Jacq.) Qvcz., commonly inhabiting forest understories, grasslands and low saline meadows, respectively. As pairs of connected ramets were subjected to reciprocal patchiness of light and nutrients, stolon connection between the two ramets significantly enhanced biomass of both ramet growing in low light intensity but high soil nutrient condition (LH ramet) and ramet growing in high light intensity but low soil nutrient condition (HL ramet) as well as whole ramet pairs (consisting of LH ramets and HL ramets). Additionally, stolon connection greatly increased root/shoot ratio of LH ramet while significantly decreased that of HL ramet. The results indicate that a reciprocal transportation of resources between interconnected ramets and a functional specialization of ramets in uptake of abundant resources occurred. By resource sharing and functional specialization, clonal plants can efficiently acquire locally abundant resources and buffer the stress caused by reciprocal patchiness of resources. 展开更多
关键词 Halerpestes ruthenica Potentilla anserina P. reptans var. sericophylla functional specialization light intensity nutrient content reciprocal patchiness of resources resource sharing
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园林地被植物——南美蟛蜞菊
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作者 伊爱娟 《农村百事通》 2004年第12期33-33,共1页
关键词 南美蟛蜞菊 园林地被植物 浙江温州 匍匐性 露地栽植 耐潮湿 全日照 栽培经验 10月份 多年生草本
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2、菖蒲
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《畜牧市场》 1995年第4期51-51,共1页
2、菖蒲菖蒲属于菖蒲属,天南星科植物。形态特征:具香气,多年生草本,可达15厘米高,通常单独密生成丛,相当木质化的根状茎常暴露在地表。叶片似鸢尾植物的叶,剑形.冥有刺激入的香味,叶长15~91厘米,扁平、光滑,边缘无... 2、菖蒲菖蒲属于菖蒲属,天南星科植物。形态特征:具香气,多年生草本,可达15厘米高,通常单独密生成丛,相当木质化的根状茎常暴露在地表。叶片似鸢尾植物的叶,剑形.冥有刺激入的香味,叶长15~91厘米,扁平、光滑,边缘无锯齿和具平行叶脉。花呈短粗棒形的花... 展开更多
关键词 菖蒲属 根状茎 形态特征 天南星科植物 茶水浸泡 美国经济 多年生草本 匍匐性 福斯特 大萧条
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Effects of Traffic Stress on the Physiological Characteristics of Agrostis stolonifera
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作者 姬承东 陈平 周芸芸 《Agricultural Science & Technology》 CAS 2015年第8期1610-1613,1680,共5页
[Objective] This study aimed to investigate the effects of traffic stress on the physiological characteristics of Agrostis stolonifera under high temperature and humidity in summer. [Method] Total 3 creeping bentgrass... [Objective] This study aimed to investigate the effects of traffic stress on the physiological characteristics of Agrostis stolonifera under high temperature and humidity in summer. [Method] Total 3 creeping bentgrass varieties (lines) were se- lected as materials, and their physiological characteristics were determined and ana- lyzed, including leaf relative water content, relative plasma membrane permeability, peroxidase activity, superoxide dismutase (SOD) activity, catalase activity, proline content and malondialdehyde (MDA) content. [Result] Under the traffic stress, the leaf relative water content, relative plasma membrane permeability, MDA content, proline content and protection enzymes activities showed significant differences among different traffic intensities and tested varieties (P〈0.05). The variations of various physiological indexes of Yuexuan 1 were smallest, and it showed relatively strong traffic tolerance. [Conclusion] Considering the traffic tolerance, the 3 tested creeping bentgrass varieties ranked as Yuexuan 1〉New Strain No.2〉Penncross. 展开更多
关键词 TRAMPLING Agrostis stolonifera Physiological characteristics
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Effect of Nitrogen Fertilizer on Growth Characteristics and Productivity of Creeping Forage Plants for Tree-Pasture Integrated System
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作者 R. Dianita L. Abdullah 《Journal of Agricultural Science and Technology(A)》 2011年第8期1118-1121,共4页
Sustainable forage production is one of the most important factors in livestock production system. Smallholder livestock production system is a part of agricultural practice in Indonesia. Limited land owned by farmers... Sustainable forage production is one of the most important factors in livestock production system. Smallholder livestock production system is a part of agricultural practice in Indonesia. Limited land owned by farmers was dominantly cultivated for food crops, and very small part of it used for cultivating forage plants. This leads to restriction of smallholder livestock development, because no more land available for forage production. Integrated forage production system by planting forage plants and food crops or trees in the same area becomes a considerable cropping system that widely practiced by the farmers in Indonesia. Some grasses and legumes have morphological advantages overcoming growth condition under shading. These growth characteristics are benefit in designing compatible system components for enhanced productivity in tree-pasture systems. Four potential shade-tolerant creeping forages that were combined with 2 levels of nitrogen application were tested using factorial completely randomized design 3 replicates. The first factor was species of creeping forages (stoloniferous): BH = Brachiaria humidicola, PN = Paspalum notatum, AC - Axonopus compressus and, AP = Arachis pintoi. The second factor was nitrogen (urea) fertilizer: A = without N fertilizer and B = with N fertilizer (300 kg Urea/ha). The observations included the growth rates of plant length, plant length, number of leaves, herbages yield (shoot), root dry weight, senescence, total N-shoot, N-soil and N-uptake. The results showed that growth characteristics and productivity of creeping forage plants was significantly differed by the species. Most species showed an increase in growth trend, except P. notatum. A. compressus significantly showed as the best performance species compared to other, in terms of growth rates, plant length, number of leaves, herbage yield (shoot) and root dry weight, N-uptake and N-shoot. This was lbllowed by B. humidicola, P. notatum, and A. pintoi. Based on previous study, A. compressus found as one of the native species in most tree system. It indicated its superior growth compared to other species tested. However, it is needed further research to observe the compatibility of each species in tree-pasture systems. 展开更多
关键词 Creeping forages growth characteristics tree-pasture system.
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