To look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellu lar allogenous nerve grafts (ANG) with chemical extraction. Methods: The doub...To look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellu lar allogenous nerve grafts (ANG) with chemical extraction. Methods: The double adhesion culture and Arab c to prohibit th e fibroblast growth were used to achieve high purified Scs. Triton x 100 and sodium deoxycholate were used to achieve ANG. Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro. The consequence was anal ysed. Results: High purified Scs and ANG were acquired, which could i ntegrate each other well. Scs could survive and transfer to aline in vitro. Conclusions: Populating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury.展开更多
文摘To look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellu lar allogenous nerve grafts (ANG) with chemical extraction. Methods: The double adhesion culture and Arab c to prohibit th e fibroblast growth were used to achieve high purified Scs. Triton x 100 and sodium deoxycholate were used to achieve ANG. Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro. The consequence was anal ysed. Results: High purified Scs and ANG were acquired, which could i ntegrate each other well. Scs could survive and transfer to aline in vitro. Conclusions: Populating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury.
