Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.Methods: Conditioned medium of ...Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.Methods: Conditioned medium of osteosarcoma cell lines (MG-63) was collected, concentrated and dialyzed. The concentrated protein was purified through gel chromatography on Sephcryl-S-100. The purified protein was tested by BMP monoclonal antibody (McAb), its molecular weight (MW) was determined by SDS-PAGE and its biological activity was demonstrated by heterotopic ossification.Results: The purified protein was proved to be BMP by BMP McAb, had a satisfactory heterotopic ossification, and its MW was about 21 kD.Conclusion: BMP existed in the conditioned medium of osteosarcoma cell and had a satisfactory biological activity after purification. Because osteosarcoma cell can be cultured and grew for a long timein vitro, this method will be helpful to a vast extraction of BMP and clinical application. Key words osteosarcoma cell - conditioned medium - bone morphogenetic protein - protein purification This project was a key scientific and technological program of Hubei Provicial Scientific and Technological Committee (No. 002p1503).展开更多
A soil batch experiment was conducted to investigate both separate and compound effects of three types of surfactants: anionic dodecylbenzene sulfonic acid sodiumsalt (DBSS), cationic cetyltrimethylammonium bromide (C...A soil batch experiment was conducted to investigate both separate and compound effects of three types of surfactants: anionic dodecylbenzene sulfonic acid sodiumsalt (DBSS), cationic cetyltrimethylammonium bromide (CTAB), and non-ionic nonyl phenol polyethyleneoxy ether (TX-100), as well as ethylenediaminetetraacetic acid (EDTA) on cadmium solubility, sorption kinetics, and sorption-desorption behavior in purple soil. The results indicated that both individual application of the three types of surfactants and surfactants combined with EDTA could stimulate Cd extraction from the soil with a general effectiveness ranking of EDTA/TX-100 > EDTA/DBSS > EDTA/CTAB > EDTA > TX-100 > DBSS > CTAB. Further study showed that the compound application of surfactants and EDTA had stronger (P < 0.05) effects on Cd solubility than those added individually. The application of surfactants and EDTA to purple soil (P < 0.05) decreased the proportion of Cd sorbed, while their effectiveness ranking was similar to that of enhanced solubilization. The sorption kinetics of Cd in purple soil was best described by the double-constant equation, while the Freundlich equation gave an excellent fit to the sorption isotherm curves. Therefore, surfactant-enhanced remediation of Cd contaminated soil is feasible and further research should be conducted.展开更多
基金This project was a key scientific and technological program of Hubei Provicial Scientific and Technological Committee (No.002p1503).
文摘Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.Methods: Conditioned medium of osteosarcoma cell lines (MG-63) was collected, concentrated and dialyzed. The concentrated protein was purified through gel chromatography on Sephcryl-S-100. The purified protein was tested by BMP monoclonal antibody (McAb), its molecular weight (MW) was determined by SDS-PAGE and its biological activity was demonstrated by heterotopic ossification.Results: The purified protein was proved to be BMP by BMP McAb, had a satisfactory heterotopic ossification, and its MW was about 21 kD.Conclusion: BMP existed in the conditioned medium of osteosarcoma cell and had a satisfactory biological activity after purification. Because osteosarcoma cell can be cultured and grew for a long timein vitro, this method will be helpful to a vast extraction of BMP and clinical application. Key words osteosarcoma cell - conditioned medium - bone morphogenetic protein - protein purification This project was a key scientific and technological program of Hubei Provicial Scientific and Technological Committee (No. 002p1503).
基金Project supported by the Foundation for University Key Teachers through the Education Committee of Chongqing,China (No. 110758).
文摘A soil batch experiment was conducted to investigate both separate and compound effects of three types of surfactants: anionic dodecylbenzene sulfonic acid sodiumsalt (DBSS), cationic cetyltrimethylammonium bromide (CTAB), and non-ionic nonyl phenol polyethyleneoxy ether (TX-100), as well as ethylenediaminetetraacetic acid (EDTA) on cadmium solubility, sorption kinetics, and sorption-desorption behavior in purple soil. The results indicated that both individual application of the three types of surfactants and surfactants combined with EDTA could stimulate Cd extraction from the soil with a general effectiveness ranking of EDTA/TX-100 > EDTA/DBSS > EDTA/CTAB > EDTA > TX-100 > DBSS > CTAB. Further study showed that the compound application of surfactants and EDTA had stronger (P < 0.05) effects on Cd solubility than those added individually. The application of surfactants and EDTA to purple soil (P < 0.05) decreased the proportion of Cd sorbed, while their effectiveness ranking was similar to that of enhanced solubilization. The sorption kinetics of Cd in purple soil was best described by the double-constant equation, while the Freundlich equation gave an excellent fit to the sorption isotherm curves. Therefore, surfactant-enhanced remediation of Cd contaminated soil is feasible and further research should be conducted.
