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工业大麻种子及苗期性别鉴定方法的研究
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作者 孙玉婷 丁美云 +5 位作者 王璐瑶 于梦迪 李珊珊 金忠民 张东向 刘丽杰 《甘肃农业大学学报》 CAS CSCD 2023年第1期130-136,共7页
【目的】探究工业大麻在生产实践中减少雄株比例,提高雌株比例的方法。【方法】通过形态学观察法、生理代谢差异比较、化学物质鉴定法及DNA分子标记鉴定法对工业大麻的种子及未进行性别分化的苗期叶片进行性别鉴定。【结果】通过测定雌... 【目的】探究工业大麻在生产实践中减少雄株比例,提高雌株比例的方法。【方法】通过形态学观察法、生理代谢差异比较、化学物质鉴定法及DNA分子标记鉴定法对工业大麻的种子及未进行性别分化的苗期叶片进行性别鉴定。【结果】通过测定雌雄工业大麻叶片中的NADH和TTCH含量发现,雌雄性工业大麻叶片的NADH和TTCH含量差异明显。通过显色反应发现,甲基红染色20 h后,雄麻变为紫红色,雌麻变为黄褐色。溴麝香草酚蓝染色12 h后,雌麻变为草绿色,雄麻变为天蓝色。快蓝B盐染色20 h后,雌叶表层纤毛变成淡红色,雄叶纤毛仍为无色透明态。通过特异性序列OPV-08和SCAR1可鉴定工业大麻种子及苗期的雌雄性。【结论】形态学观察法、生理代谢差异比较、化学物质鉴定法及DNA分子标记鉴定法(特异性序列OPV-08和SCAR1)可用于工业大麻的性别鉴定。 展开更多
关键词 工业大麻 雌雄鉴定 形态学观察 代谢差异 化学物质鉴定 分子标记鉴定
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Study on Flavonoids and Triterpenoids Content of some Euphorbiaceae Plants 被引量:1
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作者 Laila Abdel-Ghany Refahy 《Journal of Life Sciences》 2011年第2期100-107,共8页
The Spurge family (Euphorbiaceae) is a large family of flowering plants. Two plant species of this family were collected from the Egyptian desert, extracted with methanol subjected to silica gel column chromatograph... The Spurge family (Euphorbiaceae) is a large family of flowering plants. Two plant species of this family were collected from the Egyptian desert, extracted with methanol subjected to silica gel column chromatography to give six compounds, three compounds from each plant. The compounds isolated from the methanol extract of Euphorbia retusa Forssk were identified as kaempferol-3-beta-D glucopyranosyl (1), 3-O-alpha-L-xylopyranosyl (1→3)-alpha-L xylopyranosyl (1→2), beta-D glucopyranosyllup 1,12-diene-3,21-diol (2) and 5,7,8,3',4'-pentahydroxy-3-methoxyflavone (3) and the compounds isolated from Euphorbia paralais L. identified as 3,5,3'-trihydroxy-6,7-d-methoxy-4' (7"-hydroxygeranyl-1"-ether) flavones (4), 3-O-syringic acid (1→4)-alpha-L- xylopyranosyl (1→3), beta-D-rahminopyranosyl hederagenin (5) and 3-O-syringic acid (1→4), beta-D-rahminopyranosyl (1→3),beta-D-rahminopyranosyl hederagenin (6). The structure of the isolated compounds was elucidated by chemical and spectrometric analysis such as Infera red (IR), proton and carbon nuclear magnetic resonance (1H-, 13C-NMR) and chemical ionization mass spectrometry (CI-MS). 展开更多
关键词 Euphorbiaeeae Euphorbia retusa Euphorbiaparalais FLAVONOIDS TRITERPENE HEDERAGENIN lupine.
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Identification of Emergent Substances as an Indicator of the Presence of Microorganism in the Downstream of River Moraca in Locality-Vukovci
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作者 Drazana Radonj ic Marijana Krivokapic Mirjana Miloradov 《Journal of Environmental Science and Engineering(A)》 2013年第9期556-560,共5页
Based on the screening analysis for the first time in Montenegro, water samples for the presence of emergent wereanalyzed. Results from the locality Vukovci, the lower course of the river Morale, have shown the presen... Based on the screening analysis for the first time in Montenegro, water samples for the presence of emergent wereanalyzed. Results from the locality Vukovci, the lower course of the river Morale, have shown the presence of chemical components that indicate the presence of emergent in the water. Thus identification component is associated with the presence of fiziological groups of microorganisms at the site, which with its functional and significant role in many ways reduce environmental stress. 展开更多
关键词 Emergent fiziological groups of microorganisms environmental stress.
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