In the era of network live broadcasting for everyone,the development of live broadcasting platforms is also more intelligent and diversified.However,in the face of a large group of elderly users,the interface interact...In the era of network live broadcasting for everyone,the development of live broadcasting platforms is also more intelligent and diversified.However,in the face of a large group of elderly users,the interface interaction design mode used is still mainly based on the interaction mode for young groups,and is not designed for elderly users.Therefore,a design method for optimizing the interaction interface of live broadcasting platform for elderly users was proposed in this study.Firstly,the case study method and Delphi expert survey method were used to determine the design needs of elderly users and the design mode was analysed.Secondly,the orthogonal design principle was used to design a test sample of the interactive interface of live broadcasting platform applicable for the elderly users,and then a user evaluation system was established to calculate the weights of the design elements using hierarchical analysis,and then the predictive relationship between the design mode of the interactive interface of live broadcasting platform and the elderly users was established by Quantitative Theory I.Finally,Genetic Algorithm was applied to generate the optimized design scheme.The results showed that the design method based on the Genetic Algorithm and the combination of Quantitative Theory can scientifically and effectively optimize the design of the interactive interface of the live broadcasting platform for the elderly users,and improve the experience of the elderly users.展开更多
In order to further simulate the real condition of pavements during the service life,moisture is considered in long-term aging tests based on the existing researches about thermal aging and oxidation aging of asphalt....In order to further simulate the real condition of pavements during the service life,moisture is considered in long-term aging tests based on the existing researches about thermal aging and oxidation aging of asphalt.Water is injected into the pressure aging vessel(PAV)to simulate the aging process during the service life.The performance-based strategic highway research program(SHRP)parameters G/sinδ and S(t)are adopted to evaluate the high-temperature properties and low-temperature properties of the aged asphalt,respectively.The Thailand 90# asphalt,the modified Thailand 90# asphalt and the Shell 70# asphalt are used in the test.It is found that the moisture has a significant influence on parameter G/sinδ when combined with heat and oxygen,so water aging makes high-temperature performance decay more seriously.But the low-temperature performance does not change remarkably after water aging.Since the influences of temperature,oxygen and water are taken into account in the PAV test,the accuracy of asphalt aging tests can be improved and the long-term aging process of asphalt pavement can be exactly simulated.展开更多
The basic theory and effect of the new farming method of "Fenlong" cultivation which has been included in the main extension technology of Ministry of Agriculture of the People's Republic of China is fully illustra...The basic theory and effect of the new farming method of "Fenlong" cultivation which has been included in the main extension technology of Ministry of Agriculture of the People's Republic of China is fully illustrated for the first time, and it is the fourth set (generation) of farming modes and methods following manpower, animal and mechanical (tractor) farming. It follows the natural law to achieve soil activation, water saving, oxygen increase, warming and desalination through the active use of natural resources like soil, rainfall and solar energy, thereby promoting a new round of natural agricultural production and quality improvement and water con- servation, which has crop yield increase by 10%-30%, quality improvement of 5%, natural precipitation retaining increase by100%. The characteristics and mechanism are the use of spiral drill for one-time completion of the land preparation by drilling vertically to 30-50 cm of soil layer through high speed peeling. After instant high temperature and many fierce impacts, mechanical frictions, it could achieve the multiplication of the number of loose soil, soil physical modification and expansion of the soil nutrients, reservoirs, oxygen, microorganisms ("Four pools"). Fenlong cultivation can give birth to new farming culture and civilization, and it can achieve the physical "desalinized" transformation and utilization of saline soil. The formation of Fenlong green farming technology system makes it possible to invent the farming tools of "serf-propelled Fenlong machinery" that has got the patent, and it is the method for farmland (dry land, paddy field) Fenlong cultivation, saline-alkali soil smash-ridging cultivation and for the abundance of grass ecology on degraded grassland. The application of Fenlong "4+1" (arable, saline-alkali soil, grasslands, Sponge City+rivers) green development in China can achieve the "double safety" of food and living space.展开更多
Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to i...Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to identify their structures. Results Sevencompounds were isolated and their structures were identified as ferulic acid (1), conife-rylferukte(2) , bis (2-ethylhexyl) phthalate (3), dibutyl phthalate (4), lignoceric acid (5), palmitic acid(6), and Z-6, 7-cis-dihydroxyligustilide (7) Conclusion Bis (2-ethylhexyl) phthalate and dibutylphthalate were obtained from Angelica sinensis for the first time.展开更多
AIM: To study the inhibitory effects of Fuzheng Yiliuyin (Decoction for Suppressing Tumors by Strengthening the Body Resistance) in combination with chemotherapeutics on human gastric carcinoma cell strain. METHODS...AIM: To study the inhibitory effects of Fuzheng Yiliuyin (Decoction for Suppressing Tumors by Strengthening the Body Resistance) in combination with chemotherapeutics on human gastric carcinoma cell strain. METHODS: Fuzheng Yiliuyin (ZY) combined with various kinds of chemotherapeutics was put into two kinds of cultivated human gastric carcinoma cell strains, then its inhibitory effects on human gastric carcinoma cell strains were determind by the MTT method. Flow cytorneter was used to assay the apoptosis rate, and the ultrastructure of gastric carcinoma cells was observed under transmission electron microscope. RESULTS: Obvious apoptosis was seen in gastric carcinoma cells after treatment with ZY for 72 h. ZY and chemical drugs had synergistic inhibition effects on the cultivated gastric carcinoma cells, but the effects were different on various cell strains. The inhibitory effects of ZY could be strengthened by cytotoxic action and apoptosis. ZY combined with tluorouracil, etoposide and cisplatin (EFP) chemotherapeutics had better inhibitory effects on SGC-7901, while ZY combined with EFP or with DDP chemotherapeutics had better inhibitory effects than other drugs on MGC-803. CONCLUSION: ZY induces apoptosis and inhibits the growth of gastric carcinoma cells. ZY has the synergistic function of chemotherapeutics.展开更多
Hydrous magnesium oxide coated fly ash (MFA) has environmental remediation potential by providing a sub- strate for the adsorption of aqueous Cr(Ⅲ). Aqueous Cr(Ⅲ) adsorption onto MFA was examined as a function...Hydrous magnesium oxide coated fly ash (MFA) has environmental remediation potential by providing a sub- strate for the adsorption of aqueous Cr(Ⅲ). Aqueous Cr(Ⅲ) adsorption onto MFA was examined as a function of MFA dosage, pH and initial Cr(Ⅲ) concentration with the Box-Behnken approach used for experimental design and optimization using response surface methodology (RSM). pH and dosage (dosage and concentration) have significant interactive effects on Cr(Ⅲ) adsorption efficiency. Analysis of variance shows that the response surface quadratic model is highly significant and can effectively predict the experimental outcomes. Cr(Ⅲ) removal effi- ciency of 98% was obtained using optimized conditions of MFA dosage, pH and initial Cr(Ⅲ) concentration of 1,5 7 g. L- 1, 4.11 and 126 mg. L- 1, respectively. Cr(Ⅲ) adsorption onto MFA is mainly attributed to the interaction between Or(Ⅲ) and the functional group --OH of the hydrous magnesium oxide, in all probability caused by chemisorptions. The results of this study can conduce to reveal the interactions between Cr(Ⅲ) pollutant and MFA characteristics, posing important implications for the cost-effective alternative adsorption technology in the treatment of heavy metal containing wastewater.展开更多
The fractionation of moderately and highly organic phosphorus (P o) in acid soil was studied by two me thods. By the first method, after incubation for 40 d, the mineralization rates of eight constituents of stab...The fractionation of moderately and highly organic phosphorus (P o) in acid soil was studied by two me thods. By the first method, after incubation for 40 d, the mineralization rates of eight constituents of stable P o in the soil were determined. By the second method, five constituents of precipitates of stable P o in the soil were separated, then the five precipitates were put back into the original soils and incubated for 40 d and 60 d. Then, mineralization rates of the five precipitates were determined. The same results were obtained by the two methods. When the pH of the alkali solution containing stable P o was adjusted from 3.00 to 3.10, the mineralization rate of moderately stable P o was rapidly raised. Therefore, the pH 3.00 is the critical point between moderately and highly stable P o.展开更多
Irregular plates are very common structures in engineering,such as ore structures in mining.In this work,the Galerkin solution to the problem of a Kirchhoff plate lying on the Winkler foundation with two edges simply ...Irregular plates are very common structures in engineering,such as ore structures in mining.In this work,the Galerkin solution to the problem of a Kirchhoff plate lying on the Winkler foundation with two edges simply supported and the other two clamped supported is derived.Coordinate transformation technique is used during the solving process so that the solution is suitable to irregular shaped plates.The mechanical model and the solution proposed are then used to model the crown pillars between two adjacent levels in Sanshandao gold mine,which uses backfill method for mining operation.After that,an objective function,which takes security,economic profits and filling effect into consideration,is built to evaluate design proposals.Thickness optimizations for crown pillars are finally conducted in both conditions that the vertical stiffness of the foundation is known and unknown.The procedure presented in the work provides the guidance in thickness designing of complex shaped crown pillars and the preparation of backfill materials,thus to achieve the best balance between security and profits.展开更多
In this study, supported nonmetal (boron) doping TiO2 coating photocatalysts were prepared by chemical vapor deposition (CVD) to enhance the activity under visible light irradiation and avoid the recovering of TiO2. B...In this study, supported nonmetal (boron) doping TiO2 coating photocatalysts were prepared by chemical vapor deposition (CVD) to enhance the activity under visible light irradiation and avoid the recovering of TiO2. Boron atoms were successfully doped into the lattice of TiO2 through CVD, as evidenced from XPS analysis. B-doped TiO2 coating catalysts showed drastic and strong absorption in the visible light range with a red shift in the band gap transition. This novel B-TiO2 coating photocatalyst showed higher photocatalytic activity in methyl orange degradation under visible light irradiation than that of the pure TiO2 photocatalyst.展开更多
Two calculation modes for the effect of external load on slope stability, i.e., mode I in which the external load is thought to act on slope surface, and mode II in which the external load is thought to act on slip su...Two calculation modes for the effect of external load on slope stability, i.e., mode I in which the external load is thought to act on slope surface, and mode II in which the external load is thought to act on slip surface along the force action line, were considered. Meanwhile, four basic distribution patterns of external load were used, of which complex external loads could be composed. In analysis process, several limit equilibrium methods, such as Swedish method, simplified Bishop method, simplified Janbu method, Spencer method, Morgenstern-Price(M-P) method, Sarma method, and unbalanced thrust method, were also adopted to contrast their differences in slope stability under the external load. According to parametric analysis, some conclusions can be obtained as follows:(1) The external load, with the large magnitude, small inclination angle, and acting position close to the slope toe,has more positive effect on slope stability;(2) The results calculated using modes I and II of external load are similar, indicating that the calculation mode of external load has little influence on slope stability;(3) If different patterns of external loads are equivalent to each other, their slope stability under these external loads are the same, and if not, the external load leads to the better slope stability,as action position of the resultant force for external load is closer to the lower sliding point of slip surface.展开更多
AIM: To explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred i...AIM: To explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred into gastric cancer cell line MKN-45, subclone cells were obtained by persistent G_(418) selection. Cellular Smac gene expression was determined by RT-PCR and Western blotting. After treatment with mitomycin (MMC) as an apoptotic inducer, in vitro cell growth activities were investigated by trypan blue-staining method and MTT colorimetry. Cell apoptosis and its rates were determined by electronic microscopy, annexin V-FTTC and propidium iodide staining flow cytometry. Cellular caspase-3 protein expression and its activities were assayed by Western blotting and colorimetry. RESULTS: When compared with MKN-45 cells, the selected subclone cell line MKN-45/Smac had significantly higher Smac mRNA (3.12±0.21 vs 0.82±0.14, t=7.52, P<0.01) and protein levels (4.02±0.24 vs0.98±0.11, t=8.32, P<0.01). After treatment with 10 μg/mL MMC for 6-24 h, growth inhibition rate of MKN-45/Smac (15.8±1.2-54.8±2.9%) was significantly higher than that of MKN-45 (5.8±0.4-24.0±1.5%, t=6.42, P<0.01). Partial MKN-45/Smac cancer cells presented characteristic morphological changes of apoptosis under the electronic microscope with an apoptosis rate of 36.4±2.1%, which was significantly higher than that of MKN-45 (15.2±0.8%, t=9.25, P<0.01). Compared with MKN-45, caspase-3 expression levels in MKN-45/Smac were improved significantly (3.39±0.42 vs0.96±0.14, t=8.63, P<0.01), while its activities were 3.25 times as many as those of MKN-45 (0.364±0.010 vs0.112±0.007, t=6.34, P<0.01). CONCLUSION: Stable transfection of extrinsic Smac gene and its over-expression in gastric cancer cell line can significantly enhance cellular caspase-3 expression and activities, ameliorate apoptosis-inducing effects of mitomycin C on cancer cells, which is a novel strategy to improve chemotherapeutic effects on gastric cancer.展开更多
The accurate model is the most important and basic condition for the application of advanced process control, but the conventional methods do not provide satisfactory results in the case of unstable processes. To effe...The accurate model is the most important and basic condition for the application of advanced process control, but the conventional methods do not provide satisfactory results in the case of unstable processes. To effec-tively control these processes, a novel identification method (Model Parameters and Initial States Identification si-multaneously in closed loop —MPISI) is proposed. The model parameters and initial states of state equation can be simultaneously identified using this method. The results of simulation and application show that this method has the advantageous of disturbance-rejection and robustness. This method proposes a novel way for the optimization and the advanced control of the process systems.展开更多
Introducing purifiers into hydrogen network can enhance the recovery and reuse of hydrogen in refineries, further reducing the consumption of fresh hydrogen. Based on previous graphical methods, this work proposes a s...Introducing purifiers into hydrogen network can enhance the recovery and reuse of hydrogen in refineries, further reducing the consumption of fresh hydrogen. Based on previous graphical methods, this work proposes a simple and unified graphical method for integration of hydrogen networks with purification processes. Scenarios with different hydrogen concentrations of purified product can be analyzed by the unified procedure. As a result, the maximum hydrogen saved by purification reuse can he identified and the corresponding purification process can be optimized, The proposed method is easy and non-iterative, and it is valid to purification processes with any feed concentration. A conventional hydrogen network is analyzed to test the effectiveness of the proposed method.展开更多
文摘In the era of network live broadcasting for everyone,the development of live broadcasting platforms is also more intelligent and diversified.However,in the face of a large group of elderly users,the interface interaction design mode used is still mainly based on the interaction mode for young groups,and is not designed for elderly users.Therefore,a design method for optimizing the interaction interface of live broadcasting platform for elderly users was proposed in this study.Firstly,the case study method and Delphi expert survey method were used to determine the design needs of elderly users and the design mode was analysed.Secondly,the orthogonal design principle was used to design a test sample of the interactive interface of live broadcasting platform applicable for the elderly users,and then a user evaluation system was established to calculate the weights of the design elements using hierarchical analysis,and then the predictive relationship between the design mode of the interactive interface of live broadcasting platform and the elderly users was established by Quantitative Theory I.Finally,Genetic Algorithm was applied to generate the optimized design scheme.The results showed that the design method based on the Genetic Algorithm and the combination of Quantitative Theory can scientifically and effectively optimize the design of the interactive interface of the live broadcasting platform for the elderly users,and improve the experience of the elderly users.
基金The National Natural Science Foundation of China(No.50878054)
文摘In order to further simulate the real condition of pavements during the service life,moisture is considered in long-term aging tests based on the existing researches about thermal aging and oxidation aging of asphalt.Water is injected into the pressure aging vessel(PAV)to simulate the aging process during the service life.The performance-based strategic highway research program(SHRP)parameters G/sinδ and S(t)are adopted to evaluate the high-temperature properties and low-temperature properties of the aged asphalt,respectively.The Thailand 90# asphalt,the modified Thailand 90# asphalt and the Shell 70# asphalt are used in the test.It is found that the moisture has a significant influence on parameter G/sinδ when combined with heat and oxygen,so water aging makes high-temperature performance decay more seriously.But the low-temperature performance does not change remarkably after water aging.Since the influences of temperature,oxygen and water are taken into account in the PAV test,the accuracy of asphalt aging tests can be improved and the long-term aging process of asphalt pavement can be exactly simulated.
基金Supported by the National Key Technology R&D Program of China(2014BAD06B05)the Major Project of Science and Technology of Guangxi(2017AA22015)~~
文摘The basic theory and effect of the new farming method of "Fenlong" cultivation which has been included in the main extension technology of Ministry of Agriculture of the People's Republic of China is fully illustrated for the first time, and it is the fourth set (generation) of farming modes and methods following manpower, animal and mechanical (tractor) farming. It follows the natural law to achieve soil activation, water saving, oxygen increase, warming and desalination through the active use of natural resources like soil, rainfall and solar energy, thereby promoting a new round of natural agricultural production and quality improvement and water con- servation, which has crop yield increase by 10%-30%, quality improvement of 5%, natural precipitation retaining increase by100%. The characteristics and mechanism are the use of spiral drill for one-time completion of the land preparation by drilling vertically to 30-50 cm of soil layer through high speed peeling. After instant high temperature and many fierce impacts, mechanical frictions, it could achieve the multiplication of the number of loose soil, soil physical modification and expansion of the soil nutrients, reservoirs, oxygen, microorganisms ("Four pools"). Fenlong cultivation can give birth to new farming culture and civilization, and it can achieve the physical "desalinized" transformation and utilization of saline soil. The formation of Fenlong green farming technology system makes it possible to invent the farming tools of "serf-propelled Fenlong machinery" that has got the patent, and it is the method for farmland (dry land, paddy field) Fenlong cultivation, saline-alkali soil smash-ridging cultivation and for the abundance of grass ecology on degraded grassland. The application of Fenlong "4+1" (arable, saline-alkali soil, grasslands, Sponge City+rivers) green development in China can achieve the "double safety" of food and living space.
文摘Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to identify their structures. Results Sevencompounds were isolated and their structures were identified as ferulic acid (1), conife-rylferukte(2) , bis (2-ethylhexyl) phthalate (3), dibutyl phthalate (4), lignoceric acid (5), palmitic acid(6), and Z-6, 7-cis-dihydroxyligustilide (7) Conclusion Bis (2-ethylhexyl) phthalate and dibutylphthalate were obtained from Angelica sinensis for the first time.
基金Supported by TCM Administration Bureau of Shannxi Province,China, No. 199704
文摘AIM: To study the inhibitory effects of Fuzheng Yiliuyin (Decoction for Suppressing Tumors by Strengthening the Body Resistance) in combination with chemotherapeutics on human gastric carcinoma cell strain. METHODS: Fuzheng Yiliuyin (ZY) combined with various kinds of chemotherapeutics was put into two kinds of cultivated human gastric carcinoma cell strains, then its inhibitory effects on human gastric carcinoma cell strains were determind by the MTT method. Flow cytorneter was used to assay the apoptosis rate, and the ultrastructure of gastric carcinoma cells was observed under transmission electron microscope. RESULTS: Obvious apoptosis was seen in gastric carcinoma cells after treatment with ZY for 72 h. ZY and chemical drugs had synergistic inhibition effects on the cultivated gastric carcinoma cells, but the effects were different on various cell strains. The inhibitory effects of ZY could be strengthened by cytotoxic action and apoptosis. ZY combined with tluorouracil, etoposide and cisplatin (EFP) chemotherapeutics had better inhibitory effects on SGC-7901, while ZY combined with EFP or with DDP chemotherapeutics had better inhibitory effects than other drugs on MGC-803. CONCLUSION: ZY induces apoptosis and inhibits the growth of gastric carcinoma cells. ZY has the synergistic function of chemotherapeutics.
基金Supported by the State Key Development Program for Basic Research of China(2014CB460601)the International S&T Cooperation Program of China(2014DFE70070)
文摘Hydrous magnesium oxide coated fly ash (MFA) has environmental remediation potential by providing a sub- strate for the adsorption of aqueous Cr(Ⅲ). Aqueous Cr(Ⅲ) adsorption onto MFA was examined as a function of MFA dosage, pH and initial Cr(Ⅲ) concentration with the Box-Behnken approach used for experimental design and optimization using response surface methodology (RSM). pH and dosage (dosage and concentration) have significant interactive effects on Cr(Ⅲ) adsorption efficiency. Analysis of variance shows that the response surface quadratic model is highly significant and can effectively predict the experimental outcomes. Cr(Ⅲ) removal effi- ciency of 98% was obtained using optimized conditions of MFA dosage, pH and initial Cr(Ⅲ) concentration of 1,5 7 g. L- 1, 4.11 and 126 mg. L- 1, respectively. Cr(Ⅲ) adsorption onto MFA is mainly attributed to the interaction between Or(Ⅲ) and the functional group --OH of the hydrous magnesium oxide, in all probability caused by chemisorptions. The results of this study can conduce to reveal the interactions between Cr(Ⅲ) pollutant and MFA characteristics, posing important implications for the cost-effective alternative adsorption technology in the treatment of heavy metal containing wastewater.
文摘The fractionation of moderately and highly organic phosphorus (P o) in acid soil was studied by two me thods. By the first method, after incubation for 40 d, the mineralization rates of eight constituents of stable P o in the soil were determined. By the second method, five constituents of precipitates of stable P o in the soil were separated, then the five precipitates were put back into the original soils and incubated for 40 d and 60 d. Then, mineralization rates of the five precipitates were determined. The same results were obtained by the two methods. When the pH of the alkali solution containing stable P o was adjusted from 3.00 to 3.10, the mineralization rate of moderately stable P o was rapidly raised. Therefore, the pH 3.00 is the critical point between moderately and highly stable P o.
基金Project (51504044) supported by the National Natural Science Foundation of ChinaProject (14KF05) supported by the Research Fund of the State Key Laboratory of Coal Resources and Mine Safety(CUMT),China+2 种基金Project (2015CDJXY) supported by the Fundamental Research Funds for the Central Universities,ChinaProject (2015M570607) supported by Postdoctoral Science FoundationProject (2011DA105287-MS201503) supported by the Independent Subject of State Key Laboratory of Coal Mine Disaster Dynamics and Control,China
文摘Irregular plates are very common structures in engineering,such as ore structures in mining.In this work,the Galerkin solution to the problem of a Kirchhoff plate lying on the Winkler foundation with two edges simply supported and the other two clamped supported is derived.Coordinate transformation technique is used during the solving process so that the solution is suitable to irregular shaped plates.The mechanical model and the solution proposed are then used to model the crown pillars between two adjacent levels in Sanshandao gold mine,which uses backfill method for mining operation.After that,an objective function,which takes security,economic profits and filling effect into consideration,is built to evaluate design proposals.Thickness optimizations for crown pillars are finally conducted in both conditions that the vertical stiffness of the foundation is known and unknown.The procedure presented in the work provides the guidance in thickness designing of complex shaped crown pillars and the preparation of backfill materials,thus to achieve the best balance between security and profits.
基金Project (Nos. 90610005 and 20576120) supported by the National Natural Science Foundation of China
文摘In this study, supported nonmetal (boron) doping TiO2 coating photocatalysts were prepared by chemical vapor deposition (CVD) to enhance the activity under visible light irradiation and avoid the recovering of TiO2. Boron atoms were successfully doped into the lattice of TiO2 through CVD, as evidenced from XPS analysis. B-doped TiO2 coating catalysts showed drastic and strong absorption in the visible light range with a red shift in the band gap transition. This novel B-TiO2 coating photocatalyst showed higher photocatalytic activity in methyl orange degradation under visible light irradiation than that of the pure TiO2 photocatalyst.
基金Project(2015M580702)supported by the China Postdoctoral Science FoundationProject(51608541)supported by the National Natural Science Foundation of ChinaProject(2014122006)supported by the Guizhou Provincial Department of Transportation Foundation,China
文摘Two calculation modes for the effect of external load on slope stability, i.e., mode I in which the external load is thought to act on slope surface, and mode II in which the external load is thought to act on slip surface along the force action line, were considered. Meanwhile, four basic distribution patterns of external load were used, of which complex external loads could be composed. In analysis process, several limit equilibrium methods, such as Swedish method, simplified Bishop method, simplified Janbu method, Spencer method, Morgenstern-Price(M-P) method, Sarma method, and unbalanced thrust method, were also adopted to contrast their differences in slope stability under the external load. According to parametric analysis, some conclusions can be obtained as follows:(1) The external load, with the large magnitude, small inclination angle, and acting position close to the slope toe,has more positive effect on slope stability;(2) The results calculated using modes I and II of external load are similar, indicating that the calculation mode of external load has little influence on slope stability;(3) If different patterns of external loads are equivalent to each other, their slope stability under these external loads are the same, and if not, the external load leads to the better slope stability,as action position of the resultant force for external load is closer to the lower sliding point of slip surface.
文摘AIM: To explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred into gastric cancer cell line MKN-45, subclone cells were obtained by persistent G_(418) selection. Cellular Smac gene expression was determined by RT-PCR and Western blotting. After treatment with mitomycin (MMC) as an apoptotic inducer, in vitro cell growth activities were investigated by trypan blue-staining method and MTT colorimetry. Cell apoptosis and its rates were determined by electronic microscopy, annexin V-FTTC and propidium iodide staining flow cytometry. Cellular caspase-3 protein expression and its activities were assayed by Western blotting and colorimetry. RESULTS: When compared with MKN-45 cells, the selected subclone cell line MKN-45/Smac had significantly higher Smac mRNA (3.12±0.21 vs 0.82±0.14, t=7.52, P<0.01) and protein levels (4.02±0.24 vs0.98±0.11, t=8.32, P<0.01). After treatment with 10 μg/mL MMC for 6-24 h, growth inhibition rate of MKN-45/Smac (15.8±1.2-54.8±2.9%) was significantly higher than that of MKN-45 (5.8±0.4-24.0±1.5%, t=6.42, P<0.01). Partial MKN-45/Smac cancer cells presented characteristic morphological changes of apoptosis under the electronic microscope with an apoptosis rate of 36.4±2.1%, which was significantly higher than that of MKN-45 (15.2±0.8%, t=9.25, P<0.01). Compared with MKN-45, caspase-3 expression levels in MKN-45/Smac were improved significantly (3.39±0.42 vs0.96±0.14, t=8.63, P<0.01), while its activities were 3.25 times as many as those of MKN-45 (0.364±0.010 vs0.112±0.007, t=6.34, P<0.01). CONCLUSION: Stable transfection of extrinsic Smac gene and its over-expression in gastric cancer cell line can significantly enhance cellular caspase-3 expression and activities, ameliorate apoptosis-inducing effects of mitomycin C on cancer cells, which is a novel strategy to improve chemotherapeutic effects on gastric cancer.
基金Supported by the Common Project Plan of Beijing Municipal Education Commission (No.100100435).
文摘The accurate model is the most important and basic condition for the application of advanced process control, but the conventional methods do not provide satisfactory results in the case of unstable processes. To effec-tively control these processes, a novel identification method (Model Parameters and Initial States Identification si-multaneously in closed loop —MPISI) is proposed. The model parameters and initial states of state equation can be simultaneously identified using this method. The results of simulation and application show that this method has the advantageous of disturbance-rejection and robustness. This method proposes a novel way for the optimization and the advanced control of the process systems.
基金Supported by the National Basic Research Program of China(2012CB720500)the National Natural Science Foundation of China(21276204)
文摘Introducing purifiers into hydrogen network can enhance the recovery and reuse of hydrogen in refineries, further reducing the consumption of fresh hydrogen. Based on previous graphical methods, this work proposes a simple and unified graphical method for integration of hydrogen networks with purification processes. Scenarios with different hydrogen concentrations of purified product can be analyzed by the unified procedure. As a result, the maximum hydrogen saved by purification reuse can he identified and the corresponding purification process can be optimized, The proposed method is easy and non-iterative, and it is valid to purification processes with any feed concentration. A conventional hydrogen network is analyzed to test the effectiveness of the proposed method.