文献报告在药理学化疗药理教学中的应用

目的:探讨文献报告在药理学化疗药理模块教学中的应用策略和效果。方法:将四川大学2016级八年制临床医学专业共69人,分为六个组,每个组分别按照学习进度进行该部分对应文献的学习,并以幻灯加讲解形式做文献报告。全部课程完结后,采用问卷调查进行教学效果评价,并收集反馈信息。结果:文献报告能显著提高学生对学术文献相关知识、医学英语和化疗药理的掌握。结论:文献报告能提高学生的学习效果,有助于科研能力的培养。

化疗药理教学软件包及其应用

各学科的新进展和化疗新药问世周期的缩短,加剧了化疗药理授课内容与时数的矛盾。加之化疗药物内容多而散,多因素相关情况交叉而多变,无实验课辅助教学,容易枯燥或杂乱,造成教与学上的困难,为此,促使我们将计算机应用与化疗药理教学相结合,编制一套化疗药理教学软件包。一、化疗药理教学软件包的内容化疗药理教学软件包的大部分内容,是以江明性教授主编的全国统一教材《药理学》为基础,按教学大纲要求编制而成。少部分内容是参考其他药理学教材。

Effective siRNA targets screening for human telomerase reverse transcriptase

AIM: To study the inhibitory effects of siRNAs targeting different hTERT sequences and to screen the effective siRNA sequence.METHODS: Five double-stranded siRNAs targeting coding and non-coding regions of hTERT gene were designed and synthesized by T7 transcription system in vitro. siRNA4sequence was screened by full length gene targeting technique and the rest of the siRNA sequences were selected randomly. After being purified by ethanol precipitation, the siRNAs were transfected to the human hepatocellular carcinoma cell (HepG2) by Lipofectamine 2000TM. At 48-72 h after siRNAs transfection, MTT assay,RT-PCR and Western-blot were applied to evaluate the effects of siRNAs on cell growth, mRNA and protein expression level of hTERT gene, respectively.RESULTS: Compared to the control cells, the cells treated with the five double-stranded siRNAs exhibited different degrees of inhibition of cell proliferation in a dose-dependent manner. siRNA2 and siRNA4, exhibited obvious effects of inhibiting hTERT mRNA and protein expression in HepG2cells.CONCLUSION: siRNAs targeting different hTERT sequences have significantly various inhibitory effects on hTERT gene expression. The siRNA sequence screened by full length gene targeting technique has comparable inhibitory effect with the rest siRNA sequences screened by random selection, suggesting that siRNAs and antisense oligonucleic acids may have the same effective target sites. Compared with chemical synthesis method,synthesizing double-stranded siRNA by T7 transcription system in vitro is a rapid, simple, and inexpensive method suitable for screening high-effect siRNA targeting site for specific gene.

Optimizing 6-mercaptopurine and azathioprine therapy in the management of inflammatory bowel disease

The thiopurine drugs,6-mercaptopurine(6-MP) and azathioprine,are efficacious in the arsenal of inflammatory bowel disease(IBD) therapy.Previous reports indicate that 6-thioguanine nucleotide(6-TGN) levels correlate with therapeutic efficacy,whereas high 6-methylmercaptopurine(6-MMP) levels are associated with hepatotoxicity and myelotoxicity.Due to their complex metabolism,there is wide individual variation in patient response therein,both in achieving therapeutic drug levels as well as in developing adverse reactions.Several strategies to optimize 6-TGN while minimizing 6-MMP levels have been adopted to administer the thiopurine class of drugs to patients who otherwise would not tolerate these drugs due to side-effects.In this report,we will review different approaches to administer the thiopurine medications,including the administration of 6-mercaptopurine in those unsuccessfully treated with azathioprine;coadministration of thiopurine with allopurinol;co-administration of thiopurine with anti-tumor necrosis factor α;6-TGN administration;desensitization trials;and split dosing of 6-MP.

Heroin Narratives： Normalisation and Demonisation of Heroin Use at a Swedish Online Message Board

Heroin is considered potent and addictive and users are often stigmatised as the other. On the internet, however, hegemonic ideas about drugs can be resisted. This study elucidates how efforts to uormalise and demonise heroin meet at a Swedish online message board （Flashback Forum）. The study aims at analysing how heroin use is given meaning by discussants in a thread called ＂I will start with heroin＂. The data were perceived and structured as a collaborative, overarching narrative about when, why, and for whom heroin use can make sense. Discussants used stories about pleasure-seekers, miserable junkies, and self-medicating addicts to depict the activity as unproblematic and rational, problematic and irrational, or as problematic and rational. No stories about heroin use departed from the plot-lines of pleasure maximisation and pain minimisation. Results elucidate a discursive battle in which： （1） heroin use as minimisation of pain ＂wins＂ over heroin use as maximisation of pleasure; and （2） there is a general agreement that heroin use cannot be irrational and unproblematic at the same time. It is suggested that these features are central to contemporary drug discourse, and that they probably hinder public understanding of why people use heroin and therefore contribute to keep users stigmatised.

Correlation Analysis between TCM Syndromes and Physicochemical Parameters

Traditionally, differentiation of syndromes of Traditional Chinese Medicine (TCM) mainly depends on the information obtained from four diagnosis methods. Now many physicochemical parameters are available in clinic. There exists great correlation between TCM syndromes and physicochemical parameters. The objective of the paper is to analyze the correlation between TCM syndromes and physicochemical parameters quantitatively. Correlation analysis has been widely studied and many analysis methods have been developed. Mutual information based on entropy can measure arbitrary dependence between variables. It has been applied to many kinds of fields, especially to pattern recognition. But most works are restricted to discrete variables and little work has been done to study the relation between discrete and continuous variables. A novel algorithm is proposed to calculate the mutual information between discrete and continuous variables. It is used to analyze the correlation between TCM syndromes and physicochemical parameters.