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利用腺病毒载体过表达Meis 1基因能够上调化疗药对NSCLC细胞的杀伤作用 被引量:1
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作者 王娜 马德宾 +1 位作者 高蕊 刘蕾 《科学技术与工程》 北大核心 2016年第25期25-29,43,共6页
新型肿瘤抑制基因Meis 1(Myeloid ecotropic viral integration site 1)最近被证实可能具有肿瘤增殖抑制活性,是肿瘤患者预后和治疗的潜在指示分子,但其功能和作用机制尚需深入分析。考察利用腺病毒载体表达Meis 1蛋白对抗肿瘤药物杀伤N... 新型肿瘤抑制基因Meis 1(Myeloid ecotropic viral integration site 1)最近被证实可能具有肿瘤增殖抑制活性,是肿瘤患者预后和治疗的潜在指示分子,但其功能和作用机制尚需深入分析。考察利用腺病毒载体表达Meis 1蛋白对抗肿瘤药物杀伤NSCLC细胞的影响。非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞系A549感染Meis 1的腺病毒表达载体后,使用MTT、和软琼脂成集落实验验证其对NSCLC细胞增殖的抑制作用;进一步分别使用系列浓度梯度的抗肿瘤药物舒尼替尼(Sunitinib)、吉非替尼(Gefitinib)、紫杉醇(Paclitaxel)和吉西他滨(Gemcitabine)处理A549、Calu3、H460以及H358细胞,检测其抑制率,计算IC_(50)值。最后,利用耐药细胞系A549/ADR检测Meis 1逆转NSCLC化疗药多药耐药的作用。MTT和软琼脂成集落实验结果显示,Meis 1过表达能够抑制A549细胞的增殖与锚定非依赖性生长。抑制率实验显示Meis 1过表达能够上调抗肿瘤药物对NSCLC细胞的杀伤作用,显著下调其IC_(50)值。此外,Meis 1还具有逆转NSCLC细胞化疗药物多药耐药的作用。利用腺病毒载体表达MEIS1蛋白能够增加肿瘤细胞对化疗药物敏感型的作用,具有逆转肺癌细胞MDR的作用。 展开更多
关键词 非小细胞肺癌 抗肿瘤 化疗药耐药 MEIS 1基因
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苯胺类毒物在非小细胞肺癌细胞中诱导肺耐药蛋白表达和抗肿瘤药物耐受 被引量:2
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作者 车向前 马菲菲 +1 位作者 张云 林育红 《科学技术与工程》 北大核心 2018年第5期185-189,共5页
苯胺类毒物(aniline poison)是一类常用化工原料和环境污染物,通过食物或饮水污染进入体内后能够干扰人体正常内分泌系统(endocrinium)稳态,影响细胞遗传物质的稳定最终导致细胞DNA突变增加癌变或畸变的风险。最近的研究表明,苯胺类毒... 苯胺类毒物(aniline poison)是一类常用化工原料和环境污染物,通过食物或饮水污染进入体内后能够干扰人体正常内分泌系统(endocrinium)稳态,影响细胞遗传物质的稳定最终导致细胞DNA突变增加癌变或畸变的风险。最近的研究表明,苯胺类毒物还有可能影响恶性肿瘤的治疗效果。选取代表性的三个苯胺类毒物:对苯二胺(p-phenylene diamine,p-PDA)、间苯二胺(m-phenylene diamine,m-PDA)、邻苯二胺(o-phenylene diamine,o-PDA),考察其在非小细胞肺癌(non-small cell lung cancer,NSCLC)中诱导肺耐药蛋白(human lung resistance protein,LRP)表达及其可能的分子机制。培养NSCLC细胞系A549,使用系列浓度梯度的对苯二胺(p-phenylene diamine,p-PDA)、间苯二胺(m-phenylene diamine,m-PDA)、邻苯二胺(o-phenylene diamine,o-PDA)处理细胞后,使用实时荧光定量PCR(q PCR)检测LRP的mRNA表达;蛋白印记实验(Western bolt,WB)检测LRP的蛋白表达;进一步使用芳香烃受体(aryl hydrocarbon receptor,AhR)的小干扰RNA(siRNA)抑制A549细胞中AHR的表达,确定对苯二胺、间苯二胺、邻苯二胺对LRP的诱导作用是否依赖于AhR;在此基础上,使用对苯二胺、间苯二胺、邻苯二胺预处理A549细胞后,再分别使用抗肿瘤药物吉非替尼(Gefitinib)、吉西他滨(Gemcitabine)处理A549细胞,利用MTT实验检测上述抗肿瘤药物对A549细胞存活的抑制率(inhibitory rate,IR),并计算药物作用的半数抑制率(IC50值)。q PCR实验与WB实验结果显示,对苯二胺、间苯二胺、邻苯二胺刺激A549细胞能够剂量依赖地诱导LRP的mRNA、蛋白表达水平。干扰AhR表达后,该三种苯胺类毒物不能诱导LRP的mRNA与蛋白表达。MTT实验结果表明,对苯二胺、间苯二胺、邻苯二胺能够下调抗肿瘤药物吉非替尼、吉西他滨对A549细胞的杀伤作用:吉非替尼作用于A549细胞的IC50值从(1.20±0.25)μmol/L上调为(5.67±0.44)μmol/L、(6.07±0.30)μmol/L与(7.51±0.28)μmol/L,其耐药指数(resistance folds)分别为4.73、5.06与6.26;吉西他滨作用于A549细胞的IC_(50)值从(0.44±0.10)μmol/L上调至(1.55±0.25)μmol/L、(1.88±0.19)μmol/L与(2.33±0.40)μmol/L,其耐药指数分别为3.52、4.27与5.30。3种代表性苯胺类毒物能够在肺癌细胞中通过AhR诱导LRP表达,并且能够引起A549对抗肿瘤药物耐受。 展开更多
关键词 非小细胞肺癌 苯胺类毒物 抗肿瘤 化疗药耐药
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细胞凋亡抑制蛋白cIAP与卵巢癌耐药性的研究进展 被引量:4
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作者 李晨星 刘忠宇 赵恩锋 《现代生物医学进展》 CAS 2012年第18期3584-3585,3521,共3页
卵巢恶性肿瘤是女性生殖系统三大恶性肿瘤之一,其发病率在女性生殖系统肿瘤中占第三位,而死亡率却高居首位。目前对于晚期卵巢癌(ⅡI或Ⅳ期)多倾向于用新辅助化疗+肿瘤细胞减灭术+术后周期性化疗的治疗方法。但是,尽管多数患者... 卵巢恶性肿瘤是女性生殖系统三大恶性肿瘤之一,其发病率在女性生殖系统肿瘤中占第三位,而死亡率却高居首位。目前对于晚期卵巢癌(ⅡI或Ⅳ期)多倾向于用新辅助化疗+肿瘤细胞减灭术+术后周期性化疗的治疗方法。但是,尽管多数患者在最初对化疗药物较敏感,但仍有60%-80%最终死于卵巢癌,这些患者大部分都对化疗药物产生了耐药性,在更换新的化疗方案初期是有效的,但最终仍会耐药。近年来,有关细胞凋亡抑制蛋白(clAP,cellularinhibitorsofapoptosisproteins)在卵巢癌复发耐药中的作用机制的研究越来越受到重视。研究证实,clAP在耐药肿瘤细胞中呈高表达,并与多种因子共同参与形成了上皮性卵巢癌的耐药机制,抑制了化疗药物引起的肿瘤细胞的凋亡。这些发现为攻克卵巢癌的耐药机制提供了重要线索,也为卵巢癌化疗药物的应用指出了新的方向。 展开更多
关键词 卵巢癌 化疗药耐药 cIAP Smac蛋白模拟物 NF-ΚB TRAIL
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The Expression of NF-Kappa B Family Protein and Its Relationship with Drug Resistance in Breast Cancer Cell Lines
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作者 胡群 孔祥 《The Chinese-German Journal of Clinical Oncology》 CAS 2003年第4期216-218,251,252,共5页
Objective: To study the expression of NFκB family protein in breast cancer cell lines and the relationship between NFκB family protein and the drug resistance.Methods: Expression of P65, IκB-α in 14 breast cancer ... Objective: To study the expression of NFκB family protein in breast cancer cell lines and the relationship between NFκB family protein and the drug resistance.Methods: Expression of P65, IκB-α in 14 breast cancer cell lines and P50 in 11 breast cancer cell lines was detected by Western blot. The sensitivity of the cells to ADM was determined by MTT.Results: 1κB-α located mainly in the cytoplasm. P65 and P50 were in both of cytoplasm and nucleus. The expression level of P50 was higher than that of P65, especially in nucleus. MTT assay showed that IC50 was three-fold higher in the cell lines which expressed P50 in nucleus than those P50 negative in nucleus, but no difference was found in the expression of P65.Conclusion: The expression of P50 in nucleus may predict the chemotherapy resistance in breast cancer, so it can be used as an indicator to predict the prognosis. The expression of P50 is more often in breast cancer, and it may play a more important role in the chemotherapy resistance than other NFκB family members. Key words breast cancer - NFκB - chemotherapy resistance 展开更多
关键词 breast cancer NFΚB chemotherapy resistance
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Use of arrays to investigate the contribution of ATP-binding cassette transporters to drug resistance in cancer chemotherapy and prediction of chemosensitivity 被引量:7
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作者 Jian-Ting Zhang 《Cell Research》 SCIE CAS CSCD 2007年第4期311-323,共13页
Multidrug resistance (MDR) is a major problem in cancer chemotherapy. One of the best known mechanisms of MDR is the elevated expression of ATP-binding cassette (ABC) transporters. While some members of human ABC ... Multidrug resistance (MDR) is a major problem in cancer chemotherapy. One of the best known mechanisms of MDR is the elevated expression of ATP-binding cassette (ABC) transporters. While some members of human ABC transporters have been shown to cause drug resistance with elevated expression, it is not yet known whether the over-expression of other members could also contribute to drug resistance in many model cancer cell lines and clinics. The recent development ofmicroarrays and quantitative PCR arrays for expression profiling analysis of ABC transporters has helped address these issues. In this article, various arrays with limited or full list of ABC transporter genes and their use in identifying ABC transporter genes in drug resistance and chemo-sensitivity prediction will be reviewed. 展开更多
关键词 GENOMICS MDR drug resistance ABC transporter MICROARRAY real time quantitative PCR
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Research progress in the use of combinations of platinum-based chemotherapy and epidermal growth factor receptor-tyrosine kinase inhibitors
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作者 Chi Pan Suzhan Zhang Jianjin Huang 《The Chinese-German Journal of Clinical Oncology》 CAS 2013年第3期133-136,共4页
In the past decade,the advent of the epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs)has dramatically influenced the therapeutic strategies for treating lung cancer,but with tumor progression and... In the past decade,the advent of the epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs)has dramatically influenced the therapeutic strategies for treating lung cancer,but with tumor progression and drug resistance,patients will ultimately develop reduced sensitivity to EGFR-TKIs.How can we delay the emergence of drug resistance? What is the next strategy after drug resistance? How to reasonably combine platinum-based chemotherapy and EGFR-TKIs? These questions are currently the focus of lung cancer research.Clinical studies have reported that platinum-based chemotherapy can increase the sensitivity to EGFR-TKIs.However,results of pre-clinical and clinical studies have been inconsistent.The mechanisms of platinum chemotherapy and EGFR-TKIs are still unknown due to the lack of systematic research.Therefore,systematic studies are required to show the mechanisms of EGFR-TKIs and chemotherapy agents and define the markers sensitive to their combinations when given concurrently or sequentially. 展开更多
关键词 platinum-based chemotherapy epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) gefi-tinib: erlotinib
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Histone modification as a drug resistance driver in brain tumors
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作者 Guifa Xi Barbara Mania-Farnell +1 位作者 Ting Lei Tadanori Tomita 《Oncology and Translational Medicine》 2016年第5期216-226,共11页
Patients with brain tumors,specifically,malignant forms such as glioblastoma,medulloblastoma and ependymoma,exhibit dismal survival rates despite advances in treatment strategies.Chemotherapeutics,the primary adjuvant... Patients with brain tumors,specifically,malignant forms such as glioblastoma,medulloblastoma and ependymoma,exhibit dismal survival rates despite advances in treatment strategies.Chemotherapeutics,the primary adjuvant treatment for human brain tumors following surgery,commonly lack efficacy due to either intrinsic or acquired drug resistance.New treatments targeting epigenetic factors are being explored.Post-translational histone modification provides a critical regulatory platform for processes such as chromosome condensation and segregation,apoptosis,gene transcription,and DNA replication and repair.This work reviews how aberrant histone modifications and alterations in histone-modifying enzymes can drive the acquisition of drug resistance in brain tumors.Elucidating these mechanisms should lead to new treatments for overcoming drug resistance. 展开更多
关键词 histone modification drug resistance brain tumor
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卵巢癌的信息报道
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作者 申文江 《癌症康复》 2020年第3期15-17,共3页
卵巢癌是女性死亡率居第一位的恶性肿瘤。全世界每年约20万人确诊为卵巢癌,12.5万病人死于本病。尽管70%的病人初次化疗有效,可能达到缓解,但大多数终因对化疗药耐药而复发。我国卵巢癌每年新发病例5.2万,死亡2.2万。过去10年,发病率增... 卵巢癌是女性死亡率居第一位的恶性肿瘤。全世界每年约20万人确诊为卵巢癌,12.5万病人死于本病。尽管70%的病人初次化疗有效,可能达到缓解,但大多数终因对化疗药耐药而复发。我国卵巢癌每年新发病例5.2万,死亡2.2万。过去10年,发病率增长了30%,死亡率增长了18%。 展开更多
关键词 卵巢癌 新发病例 初次化疗 恶性肿瘤 化疗药耐药 死亡率 发病率
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Hollow iron oxide nanoparticles as multidrug resistant drug delivery and imaging vehicles 被引量:11
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作者 Ruijun Xing Ashwinkumar A. Bhirde +4 位作者 Shouju Wang Xiaolian Sun Gang Liu Yanglong Hou Xiaoyuan Chen 《Nano Research》 SCIE EI CAS CSCD 2013年第1期1-9,共9页
Magnetic nanopartides have been used as drug delivery vehicles against a number of cancer cells. Most of these theranostic formulations have used solid iron oxide nanoparticles (SIONPs) loaded with chemotherapeutics... Magnetic nanopartides have been used as drug delivery vehicles against a number of cancer cells. Most of these theranostic formulations have used solid iron oxide nanoparticles (SIONPs) loaded with chemotherapeutics as nano-carrier formulation for both magnetic resonance imaging (MRI) and cancer therapy. In this study, we applied the dopamine-plus-human serum albumin (HSA) method to modify hollow iron oxide nanoparticles (HIONPs) and encapsuated doxorubicin (DOX) within the hollow porous structure of the nano-carrier. The new delivery system can load more drug than solid iron oxide nanoparticles of the same core size using the same coating strategy. The HIONPs-DOX formulation also has a pH-dependent drug release behaviour. Compared with free DOX, the HIONPs-DOX were more effectively uptaken by the multidrug resistant OVCAR8- ADR cells and consequently more potent in killing drug resistant cancer cells. MRI phantom and cell studies also showed that the HIONPs-DOX can decrease the T2 MRI signal intensity and can be used as a MR/contrast agent while acting as a drug delivery vehicle. For the first time, the dual application of chemo drug transport and MR imaging using the HIONPs-DOX formulation was achieved against both DOX-sensitive and DOX-resistant cancer cells. 展开更多
关键词 drug resistance hollow nanoparticles DOXORUBICIN magnetic resonanceimaging (MRI) drug delivery
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