目的:建立紫杉醇诱发大鼠周围神经疾病的模型,研究α-硫辛酸对紫杉醇诱发大鼠周围神经疾病的治疗作用。方法:40只SD大鼠随机平均分为紫杉醇组(P组)、对照组(C组)、预治疗组(AP组)和后治疗组(PA组)。四组分别隔日给予紫杉醇2mg/kg(P组、A...目的:建立紫杉醇诱发大鼠周围神经疾病的模型,研究α-硫辛酸对紫杉醇诱发大鼠周围神经疾病的治疗作用。方法:40只SD大鼠随机平均分为紫杉醇组(P组)、对照组(C组)、预治疗组(AP组)和后治疗组(PA组)。四组分别隔日给予紫杉醇2mg/kg(P组、AP组和PA组)或等量生理盐水(C组)ip,共4次。AP组和PA组分别于D0-D6和D21-D27给予α-硫辛酸25 mg/kg(ip,qd)。D0、D7、D14、D21和D28分别测量大鼠的机械痛阈和热痛阈、脊髓背角NF-κB的表达及星型胶质细胞的数量、脊髓背角和背根神经节中NF-κB的含量。结果:P组D14和D21的热痛阈显著低于C组,4g和15 g von Frey纤维刺激缩足反应显著高于C组。AP组D14和D21的热痛阈显著高于P组,4 g和15 g von Frey纤维刺激缩足反应显著低于P组。P组脊髓背角中星型胶质细胞的数量显著低于C组。AP组脊髓背角中星型胶质细胞的数量显著高于P组。C组和AP组NF-κB主要在脊髓背角星型胶质细胞的细胞浆中表达。P组和PA组NF-κB在脊髓背角星型胶质细胞的细胞核和细胞浆内同时表达。四组NF-κB在脊髓和背根神经节内的表达差异无显著意义。结论:预防性给予α-硫辛酸对紫杉醇诱发大鼠周围神经疾病模型中的热痛阈下降、痛觉过敏、痛觉超敏的减少,脊髓背角星型胶质细胞数量增加和NF-κB异常激活的抑制。展开更多
AIM： To study the inhibitory effect of mononuclear bone marrow cell （BNC） transplantation on carbon tetrachloride （CCl4） -induced liver fibrosis in rats. METHODS： Rat liver fibrosis models were induced by CCl4 a...AIM： To study the inhibitory effect of mononuclear bone marrow cell （BNC） transplantation on carbon tetrachloride （CCl4） -induced liver fibrosis in rats. METHODS： Rat liver fibrosis models were induced by CCl4 and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group （n = 10） and control group （n = 10）. BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl4 was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Uver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis （hydroxyproline and collagen fibre） and liver pathological grades in rats were evaluated. RESULTS： The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6± 128.8 μg/g in treatment group, and 596.0 ± 341.8 μg/g in control group. The percentage of collagen fibre was 3.75% ± 0.98% in treatment group and 5.02% ± 0.44% in control group. There was a significant difference between the two groups （P 〈 0.05）. Liver pathological grade decreased from grade N to grade 11 partially in treatment group （P 〈 0.05） with no obvious improvement in control group （P 〉 0.05）. There was a significant difference between treatment group and control group （P 〈 0.05）.CONCLUSION： Transplantation of BMC can improve liver fibrosis due to chronic liver injury in rats.展开更多
Objective： To observation the therapeutic effect and the adverse reaction of preoperative induced hypertension chemotherapy （IHC） on the Ⅲ staging cardia or fundus of stomach carcinoma. Methods： 49 cases of the ...Objective： To observation the therapeutic effect and the adverse reaction of preoperative induced hypertension chemotherapy （IHC） on the Ⅲ staging cardia or fundus of stomach carcinoma. Methods： 49 cases of the Ⅲ staging cardia or fundus of stomach carcinoma were divided into two groups at random. The observation group included 19 cases and the control group included 30 cases. Every case used one cycle chemotherapy. The observation group （IHC group） used chemotherapy and AT Ⅱ. The control group （simple chemotherapy group） used simple chemotherapy. All cases of the two groups operated after 3 weeks rest. The specimen＇s DNA was analyzed by flow cytometry. Results： The effective power of observation group was 63.2% （12/19）, exairesis ratio was 84.2% （16/19）. The effective power of control group was 30% （9/30）, exairesis ratio was 63.3% （19/30）. DNA ploid determination： 13 cases were diploid and 5 cases were heteropioid in the observation group, 9 cases were diploid and 16 cases were heteroploid in the control group. The operative complications and risks of the two groups did not increase. Conclusion： IHC can increase the therapeutic effect of Ⅲ staging cardia or fundus of stomach carcinoma obviously. IHC is one of the chemotherapy methods that have good future, it has good value of clinic enlarge trial.展开更多
Objective： The aim of our study was to evaluate the toxicity and efficacy of induction chemotherapy （ICT） followed by three-dimensional conformal radiotherapy （3D CRT） and concurrent weekly paclitaxel on unresect...Objective： The aim of our study was to evaluate the toxicity and efficacy of induction chemotherapy （ICT） followed by three-dimensional conformal radiotherapy （3D CRT） and concurrent weekly paclitaxel on unresectable non-small cell lung cancer （NSCLC）. Methods： Stage III NSCLC patients with favorable conditions were treated with 2 to 4 cycles of carboplatin （AUC = 5-6, dl） combined with paclitaxel （175 mg/m〈 dl）, then followed by weekly paclitaxel （40 mg/m2） and concurrent 3D CRT within 3-4 weeks. The prescription dose was given as high as possible under the condition that V20 〈 31% and spinal cord dose 〈 50 Gy. Results： Thirty-one patients were enrolled. ICT was well tolerated. During the concurrent chemoradiotherapy, the treatment of 3 patients was ended ahead of the schedule because of severe pulmonary and heart toxicities; the treatment of 2 patients was delayed for 7 and 12 days because of fatigue. Myelosuppression was mild （16/31）： all were grade 1-2 except 1 was grade 3. Lymphocytopenia was more obvious （29/31, grade 3 in 21）. Three patients developed grade 3 radiation-induced esophagitis, and 2 developed grades 3-4 radiation-induced pneumonitis. Two developed grade 3 esophageal stricture. No grades 3-4 pulmonary fibrosis was observed. The overall response rate was 74.1%. The 1-, 2-, 3-year overall survival rates were 74.2%, 41.9%, and 34.6%, respectively, with the median survival time of 18.5 months. The 1-, 2-, 3-year local progression-freely survival rates were 64.5%, 32.3%, and 20.5%, respectively, with the median local progression-freely survival time of 14.3 months. Conclusion： The program of ICT followed by weekly paclitaxel and 3D CRT is accomplished in most of the favorable stage III NSCLC patients. The toxicity is tolerable, and the response rate is inspiriting.展开更多
Lung cancer is the leading cause of death due to cancer in China. In recent years, great progress has been made in radiotherapy for lung cancer patients in China. The main advance- ments include the following aspects...Lung cancer is the leading cause of death due to cancer in China. In recent years, great progress has been made in radiotherapy for lung cancer patients in China. The main advance- ments include the following aspects： （1） stereotactic ablative radiotherapy for early stage non- small cell lung cancer （NSCLC）, （2） post-operative radiotherapy for NSCLC, （3） combined chemotherapy and radiotherapy for locally advanced NSCLC, （4） improved radiotherapy for advancedNSCLC, and 5） orediction of radiation-induced luna toxicitv.展开更多
围绝经期泌尿生殖综合征(genitourinary syndrome of menopause,GSM),在绝经后妇女中的发病率约50%以上,而在乳腺癌患者中的发病率可高达79%~95%,且症状常较正常绝经女性更严重,显著降低了患者的生活质量。此现象并未受到充分的重视,其...围绝经期泌尿生殖综合征(genitourinary syndrome of menopause,GSM),在绝经后妇女中的发病率约50%以上,而在乳腺癌患者中的发病率可高达79%~95%,且症状常较正常绝经女性更严重,显著降低了患者的生活质量。此现象并未受到充分的重视,其中多数患者并未被诊断和进一步治疗。研究发现,乳腺癌化疗、内分泌治疗均可能增加GSM的发生率及其严重程度。因此,正确认识乳腺癌患者中的GSM,并探索其有效防治措施,对改善患者生活质量及预后具有重要意义。展开更多
文摘目的:建立紫杉醇诱发大鼠周围神经疾病的模型,研究α-硫辛酸对紫杉醇诱发大鼠周围神经疾病的治疗作用。方法:40只SD大鼠随机平均分为紫杉醇组(P组)、对照组(C组)、预治疗组(AP组)和后治疗组(PA组)。四组分别隔日给予紫杉醇2mg/kg(P组、AP组和PA组)或等量生理盐水(C组)ip,共4次。AP组和PA组分别于D0-D6和D21-D27给予α-硫辛酸25 mg/kg(ip,qd)。D0、D7、D14、D21和D28分别测量大鼠的机械痛阈和热痛阈、脊髓背角NF-κB的表达及星型胶质细胞的数量、脊髓背角和背根神经节中NF-κB的含量。结果:P组D14和D21的热痛阈显著低于C组,4g和15 g von Frey纤维刺激缩足反应显著高于C组。AP组D14和D21的热痛阈显著高于P组,4 g和15 g von Frey纤维刺激缩足反应显著低于P组。P组脊髓背角中星型胶质细胞的数量显著低于C组。AP组脊髓背角中星型胶质细胞的数量显著高于P组。C组和AP组NF-κB主要在脊髓背角星型胶质细胞的细胞浆中表达。P组和PA组NF-κB在脊髓背角星型胶质细胞的细胞核和细胞浆内同时表达。四组NF-κB在脊髓和背根神经节内的表达差异无显著意义。结论:预防性给予α-硫辛酸对紫杉醇诱发大鼠周围神经疾病模型中的热痛阈下降、痛觉过敏、痛觉超敏的减少,脊髓背角星型胶质细胞数量增加和NF-κB异常激活的抑制。
基金the Key Scientific and Technological Projects of Guangdong Province, No. 2005A30201007Guangdong Provincial Natural Science Fundation, No. 06021343
文摘AIM： To study the inhibitory effect of mononuclear bone marrow cell （BNC） transplantation on carbon tetrachloride （CCl4） -induced liver fibrosis in rats. METHODS： Rat liver fibrosis models were induced by CCl4 and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group （n = 10） and control group （n = 10）. BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl4 was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Uver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis （hydroxyproline and collagen fibre） and liver pathological grades in rats were evaluated. RESULTS： The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6± 128.8 μg/g in treatment group, and 596.0 ± 341.8 μg/g in control group. The percentage of collagen fibre was 3.75% ± 0.98% in treatment group and 5.02% ± 0.44% in control group. There was a significant difference between the two groups （P 〈 0.05）. Liver pathological grade decreased from grade N to grade 11 partially in treatment group （P 〈 0.05） with no obvious improvement in control group （P 〉 0.05）. There was a significant difference between treatment group and control group （P 〈 0.05）.CONCLUSION： Transplantation of BMC can improve liver fibrosis due to chronic liver injury in rats.
基金a grant from the Science Foundation of Handan City (No. 02115-3).
文摘Objective： To observation the therapeutic effect and the adverse reaction of preoperative induced hypertension chemotherapy （IHC） on the Ⅲ staging cardia or fundus of stomach carcinoma. Methods： 49 cases of the Ⅲ staging cardia or fundus of stomach carcinoma were divided into two groups at random. The observation group included 19 cases and the control group included 30 cases. Every case used one cycle chemotherapy. The observation group （IHC group） used chemotherapy and AT Ⅱ. The control group （simple chemotherapy group） used simple chemotherapy. All cases of the two groups operated after 3 weeks rest. The specimen＇s DNA was analyzed by flow cytometry. Results： The effective power of observation group was 63.2% （12/19）, exairesis ratio was 84.2% （16/19）. The effective power of control group was 30% （9/30）, exairesis ratio was 63.3% （19/30）. DNA ploid determination： 13 cases were diploid and 5 cases were heteropioid in the observation group, 9 cases were diploid and 16 cases were heteroploid in the control group. The operative complications and risks of the two groups did not increase. Conclusion： IHC can increase the therapeutic effect of Ⅲ staging cardia or fundus of stomach carcinoma obviously. IHC is one of the chemotherapy methods that have good future, it has good value of clinic enlarge trial.
文摘Objective： The aim of our study was to evaluate the toxicity and efficacy of induction chemotherapy （ICT） followed by three-dimensional conformal radiotherapy （3D CRT） and concurrent weekly paclitaxel on unresectable non-small cell lung cancer （NSCLC）. Methods： Stage III NSCLC patients with favorable conditions were treated with 2 to 4 cycles of carboplatin （AUC = 5-6, dl） combined with paclitaxel （175 mg/m〈 dl）, then followed by weekly paclitaxel （40 mg/m2） and concurrent 3D CRT within 3-4 weeks. The prescription dose was given as high as possible under the condition that V20 〈 31% and spinal cord dose 〈 50 Gy. Results： Thirty-one patients were enrolled. ICT was well tolerated. During the concurrent chemoradiotherapy, the treatment of 3 patients was ended ahead of the schedule because of severe pulmonary and heart toxicities; the treatment of 2 patients was delayed for 7 and 12 days because of fatigue. Myelosuppression was mild （16/31）： all were grade 1-2 except 1 was grade 3. Lymphocytopenia was more obvious （29/31, grade 3 in 21）. Three patients developed grade 3 radiation-induced esophagitis, and 2 developed grades 3-4 radiation-induced pneumonitis. Two developed grade 3 esophageal stricture. No grades 3-4 pulmonary fibrosis was observed. The overall response rate was 74.1%. The 1-, 2-, 3-year overall survival rates were 74.2%, 41.9%, and 34.6%, respectively, with the median survival time of 18.5 months. The 1-, 2-, 3-year local progression-freely survival rates were 64.5%, 32.3%, and 20.5%, respectively, with the median local progression-freely survival time of 14.3 months. Conclusion： The program of ICT followed by weekly paclitaxel and 3D CRT is accomplished in most of the favorable stage III NSCLC patients. The toxicity is tolerable, and the response rate is inspiriting.
文摘Lung cancer is the leading cause of death due to cancer in China. In recent years, great progress has been made in radiotherapy for lung cancer patients in China. The main advance- ments include the following aspects： （1） stereotactic ablative radiotherapy for early stage non- small cell lung cancer （NSCLC）, （2） post-operative radiotherapy for NSCLC, （3） combined chemotherapy and radiotherapy for locally advanced NSCLC, （4） improved radiotherapy for advancedNSCLC, and 5） orediction of radiation-induced luna toxicitv.
文摘围绝经期泌尿生殖综合征(genitourinary syndrome of menopause,GSM),在绝经后妇女中的发病率约50%以上,而在乳腺癌患者中的发病率可高达79%~95%,且症状常较正常绝经女性更严重,显著降低了患者的生活质量。此现象并未受到充分的重视,其中多数患者并未被诊断和进一步治疗。研究发现,乳腺癌化疗、内分泌治疗均可能增加GSM的发生率及其严重程度。因此,正确认识乳腺癌患者中的GSM,并探索其有效防治措施,对改善患者生活质量及预后具有重要意义。
