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火焰原子吸收光谱法间接测定碘 被引量:28
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作者 孙汉文 孙智敏 张德强 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2000年第4期537-539,共3页
在弱酸性介质中 ,I- 与Cu+生成沉淀 ,经硫代硫酸钠溶解后 ,以火焰原子吸收法测定铜 ,从而间接求得碘的含量。本方法的线性范围为 0~ 80 0 μg·mL- 1 ,回收率为 99 9%~ 1 0 0 3 %,相对标准偏差为 0 7%。将方法应用于碘盐、华素... 在弱酸性介质中 ,I- 与Cu+生成沉淀 ,经硫代硫酸钠溶解后 ,以火焰原子吸收法测定铜 ,从而间接求得碘的含量。本方法的线性范围为 0~ 80 0 μg·mL- 1 ,回收率为 99 9%~ 1 0 0 3 %,相对标准偏差为 0 7%。将方法应用于碘盐、华素片、紫菜中碘的测定 ,结果满意。 展开更多
关键词 间接测定 原子吸收光谱法 碘盐 化素片 紫菜
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普通针刺配合吡拉西坦脑梗死后遗症70例疗效观察
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作者 安志强 《中文科技期刊数据库(引文版)医药卫生》 2020年第5期125-126,共2页
本次研究主要针对针刺配合吡拉西坦治疗脑梗死后遗症患者的临床疗效,展开分析和研究。方法:随机选取我院收录的脑梗死后遗症患者共计70名,作为本次针刺配合吡拉西坦治疗脑梗死后遗症的临床基本研究资料。使用先后入院顺序和随机分组的形... 本次研究主要针对针刺配合吡拉西坦治疗脑梗死后遗症患者的临床疗效,展开分析和研究。方法:随机选取我院收录的脑梗死后遗症患者共计70名,作为本次针刺配合吡拉西坦治疗脑梗死后遗症的临床基本研究资料。使用先后入院顺序和随机分组的形式,将此70名脑梗死后遗症患者均匀分成观察组和对照组。其中对照组的脑梗死后遗症患者采取的是吡拉西坦进行治疗,而观察组的35名脑梗死后遗症患者,则是采取针刺配合吡拉西坦进行治疗,在经过一段时间的治疗之后,对比两组脑梗死后遗症患者的临床疗效。结果:在治疗总有效率方面,观察组是明显的比对照组要优秀了许多,而且P<0.05,拥有统计学意义存在;在神经功能缺损程度的评分对比方面,观察组的35名脑梗死后遗症患者的评分明显是低于对照组患者的评分,而且P<0.05,拥有统计学意义存在;在治疗后血流动力学指标检测值的对比方面,观察组是明显的比对照组要优秀了许多,而且P<0.05,拥有统计学意义存在。结论:针对脑梗死后遗症患者,给予针刺配合吡拉西坦进行临床治疗,可以有效的改善患者的临床病症,全面减轻脑梗死后遗症患者的神经功能损伤程度,进一步促进患者的肢体功能尽快的复原,可以说此方法对患者的生活质量提升,拥有极大的重要意义存在。 展开更多
关键词 针灸 灯盏化素片 脑梗死后遗症 生活质量
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A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis 被引量:8
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作者 王家丰 许飞 +1 位作者 李莉 张国范 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第2期419-425,共7页
The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among... The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetie studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I (COI) of five common Crassostrea species in China (including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas) and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers (Oyster- COI-1), we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species. 展开更多
关键词 identification cytochrome oxidase subunit I (COl) CRASSOSTREA OYSTER high-resolution melting (HRM)
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Genetic identification of two species of Pleuronichthys by DNA barcoding
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作者 张辉 张岩 +2 位作者 高天翔 李鹏飞 徐汉祥 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第5期967-972,共6页
DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar specie... DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding (cytochrome c oxidase subunit I (COI)), the mtDNA control region and cytochrome b. The results reveal that: l) intraspecific variation in the DNA barcode is much less than interspecific variation; 2) the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3) COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments. 展开更多
关键词 Pleuronichthys DNA barcoding COI CR Cyt b
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