温郁金提取物对胰腺纤维化模型大鼠血清羟脯氨酸的影响

目的观察温郁金用JET醇提取物，对二乙基二硫代氨基甲酸钠（DDC）诱导的胰腺纤维化大鼠模型血清羟脯氨酸（Hyp）的影响。方法66只雄性、健康、清洁级SD大鼠，随机分为空白对照组（A组，／1,=12）、模型组（B组，17,=18）、温郁金正丁醇提取物高剂量组（C组，n=18）、温郁金正丁醇提取物低剂量组（D组，n=18）。除空白对照组外，均经腹腔注射DDC750mg／kg，制备胰腺纤维化CP大鼠模型。空白对照组大鼠在正常饲养1周后，每天予以生理盐水灌胃（每次1ml／kg，1次／d）；模型组在造模1周后，在造模的同时每天1次予以生理盐水1ml／kg灌胃；C组在造模1周后，在造模的同时每天1次予以温郁金生药5g／kg提取物灌胃；D组在造模1周后，在造模的同时每天1次予以温郁金生药1．25g／kg提取物灌胃。至第10周，麻醉大鼠，腹主动脉取血，用ELISA法测定血清Hyp水平。结果血清Hyp水平，空白对照组（21．37±4．94）ug／L，模型组（34．03±15．87）斗∥L，C组（23．504-12．67）ug∥L，D组（22．66±10．65）ug／L，模型组血清Hyp水平高于空白对照组（P〈0．05），c组、D组低于空白对照组（P〈0．05），C组、D组、空白对照三组间差异无统计学意义。结论温郁金用正丁醇提取物能降低DDC诱导的胰腺纤维化大鼠的血清Hvo水平。

IL-28B在实验性肺纤维化小鼠肺组织内的动态表达

目的:探讨白介素28B(IL-28B)在肺纤维化发病中的表达及意义。方法:健康昆明种小鼠48只,随机分成实验组和对照组,每组24只。实验组:气管内一次性注入生理盐水稀释的博来霉素(5mg/kg);对照组:气管内注入生理盐水0.06～0.07mL。分别于给药后第7、14、21、28天,每组各处死小鼠6只。无菌操作下取出小鼠肺组织,病理学方法观察各阶段肺内炎症及纤维化程度;检测肺组织内羟脯氨酸的含量,Westernblot检测肺内IL-28B的蛋白表达。结果:实验组肺组织病理切片按时间顺序呈现由肺泡炎至纤维化动态改变,实验组肺泡炎和纤维化程度均高于对照组(均P<0.01)。实验组肺组织内羟脯氨酸在第14～28天明显高于对照组(P<0.01)。实验组第7、14、21天的IL-28B的蛋白表达也高于对照组(P<0.05或P<0.01);IL-28B表达高峰在第14天,此后逐渐下降,第28天时表达水平与对照组差异无统计学意义(P>0.05)。结论:IL-28B在致肺纤维化损伤中发挥重要作用,检测IL-28B的水平对肺纤维化的早期诊断有帮助。

Effects of polypeptide from Chlamys farreri on amino acid con-tent in guinea pig skin irradiated by chronic ultraviolet A and B

We examined the effects of polypeptide from Chlamys farreri (PCF) on the amount of hy-droxyproline in guinea pig skin irradiated by chronic ultraviolet A (UVA) and ultraviolet B (UVB) radiation. PCF was applied locally before repeated exposure of guinea pig to UVA and UVB. The contents of hy-droxyproline and other amino acids in guinea pig skin were determined by automatic amino acid analyzer. Our results showed that: (1) long-time UVA and UVB radiation can reduce dramatically the amounts of hy-droxyproline, aspartic acid, threonine, glycine, phenylalanine and lysine in guinea pig skin in comparison with the control group (P < 0.05); (2) Compared with model group, pre-treatment with 5 % and 20 % PCF prior to UVA and UVB radiation can inhibit the decline of amino acids content in guinea pig skin in a dose-dependent manner (P < 0.05). As the decrease of hydroxyproline, glycine and lysine contents in the skin directly reflexes type I collagen degeneration, our results indicated that the chronic application of PCF can protect skin type I collagen against UV radiation, and thus protect skin from photoaging.

Effects of Acupuncture and Moxibustion on MDA, GSH-Px, and Hyp in the Skin of Senile Mice

Objective： To observe the effects of acupuncture and moxibustion on the skin histomorphology of senile mice. Methods： The subacute senile model of mouse was made by subcutaneous injection of D-galactose into the neck, then they were randomly allocated into an acupuncture group, a moxibustion group, a model group, and a blank group, and measured the content of malondialdehyde （MDA）, activity of glutathione peroxidase （GSH-Px）, and content of hydroxyproline （Hyp） in the skin tissue. Results： Acupuncture and moxibustion at Zusanli （ST 36） could decrease the content of MDA, enhance the activity of GSH-Px, and increase the content of Hyp in the skin tissue of senile mice. Conclusion： Acupuncture and moxibustion at Zusanli （ST 36） could delay the senescence progress of mice＇s skin.

Experimental Research of Acupuncture Therapy on Skin Aging in Senile Rats

Objective： To observe the effect of acupuncture on skin aging of senile rats and discuss the mechanism of acupuncture in treating skin aging. Methods： The 18-month-old senile Wistar rats were randomly divided into three groups, a blank group, a surrounding needling group, a surrounding needling plus Zusanli （ST 36） group, comparing with the 8-month-old young rats. The tests included the content of dissoluble hydroxyproline （Hyp） and the activity of superoxide dismutase （SOD） in the skin of rats＇ abdomen. The transmission electron microscope was used for the morphological observation of the fibroblasts and the arrangement of the collagen fiber. Results： The dermal organelles in the fibroblasts of the rats decreased with the cellular structures. Meanwhile, the spaces among the collagen fibers extended and the aging bridging increased. The surrounding needling improved the activity of the fibroblasts. The dissoluble Hyp content in the surrounding needling group was much higher than the rats in the blank group, but the SOD activity didn＇t change significantly. However, the surrounding needling plus Zusanli （ST 36） enhanced the SOD activity. Conclusion： Acupuncture may improve the activity of the fibroblasts in the skin to increase the dissoluble collagen content, resulting in the improvement of an aged appearance. Surrounding needling plus needling Zusanli （ST 36） could enhance the SOD activity.

A new facile approach to N-alkylpyrroles from direct redox reaction of 4-hydroxy-L-proline with aldehydes

An unprecedented acetic acid-catalyzed efficient access to N-alkylpyrroles from reaction of 4-hydroxy-L-proline with a variety of aldehydes has been achieved in good to excellent yields under mild reaction conditions.