Comparison of Nucleic Acid Content among Non-diapause Pupae, Diapause Pupae and Eclosion-adult from Diapause Pupae of Papilio memnon

[Objective] The aim of this study was to provide basis for deeply understanding the diapause mechanism of Papilio memnon L. [Method] RNA and DNA content of non-diapause pupae, diapause pupae and eclosion-adult from diapause pupae at different development stages were detected by the colorimetry. [Result] RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg, while diapause pupae was 4.326 0-5.885 3 μg/mg and eclosion-adult from diapause pupae was 20.779 3 μg/mg at initial stage. DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg, while diapause pupae was 0.452 0-0.828 3 μg/mg and eclosion-adult from diapause pupae was 1.727 0 μg/mg at initial stage. [Conclusion] The nucleic acid content and change is related to the development stage.

利福平对黑腹果蝇种群的影响

黑腹果蝇是危害蓝莓果实最严重的害虫之一,成虫将卵产在蓝莓果皮下,卵孵化后以幼虫为害,严重影响蓝莓的鲜销、贮藏及加工。为了探索抗生素利福平对黑腹果蝇种群的影响,以黑腹果蝇为试材,饲喂不同浓度(0.04、0.08、0.16、0.32、0.64、1.28、2.56mg·mL^(-1))的利福平,筛选出适宜浓度(0.64mg·mL^(-1))利福平并对黑腹果蝇F0代和F1代进行深入研究,以期为黑腹果蝇的种群繁殖提供参考依据。结果表明:使用利福平的浓度高于0.64mg·mL^(-1)时,黑腹果蝇产卵量显著(P<0.05)下降,而低浓度的利福平则对黑腹果蝇没有显著影响。在F0代中,利福平处理雌性黑腹果蝇(C0)后,子代雌虫发育历期比对照显著增加(P<0.05),处理雄性黑腹果蝇(B0)后化蛹量较处理雌性(C0)和处理雌雄性黑腹果蝇(D0)均显著增加(P<0.05)。F1代中,处理B1和C1后,化蛹量、羽化量、发育历期和雌性比均显著(P<0.01)变化,在D1处理中,黑腹果蝇产卵量极显著下(P<0.01),从14.47降至1.93,化蛹量和羽化量均急剧降低,不能发育成完整的成虫。结果表明,高于0.64mg·mL^(-1)的利福平能降低黑腹果蝇F1子代的产卵量,使其种群下降。