Applications of Three DNA Barcodes in Assorting Intertidal Red Macroalgal Flora in Qingdao, China

This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI（cytochrome oxidase subunit I） are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA （universal plastid amplicon, domain V of 23S rRNA）, and ITS （nuclear internal transcribed spacer） were employed to analyze common species of intertidal red seaweeds in Qingdao （119.3°-121°E, 35.35°-37.09°N）. The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification.

Narratives of Spatial Division： The Role of Social Memory in Shaping Urban Space in Belfast

The paper examines the role of shared spaces in divided cities in promoting future sustainable communities and spaces described as inclusive to all. It addresses the current challenges that prevent such inclusiveness and suggests future trends of its development to be of benefit to the wider city community. It explains how spaces in divided cities are carved up into perceived ownerships and territorialized areas, which increases tension on the shared space between territories; the control of which can often lead to inter-community disputes. The paper reports that common shared space in-between conflicting communities takes on increased importance since the nature of the conflict places emphasis on communities＇ confidence, politically and socially, while also highlighting the necessity for confidence in inclusion and feeling secure in the public domain. In order to achieve sustainable environments, strategies to promote shared spaces require further focus on the significance of everyday dynamics as essential aspects for future integration and conflict resolution.

Genetic Diversity Analysis of Balsam Pear(Momordica charantia L.) by ISSR Marker

[Objective] The aim was to explore the relationship between the classification and regional distribution of balsam pear. [Method] In the research, 30 varieties of balsam pear were analyzed by ISSR marker. [Result] The research showed that172 bands were amplified by 18 primers, in which, 132 bands were polymorphism and the polymorphic proportion was 76.7%. All of the samples can be divided into four categories by UPGMA analysis. [Conclusion] The results indicated that the classification of balsam pear is similar as varieties' regional distribution, which will be useful for genetic relationships analysis and parents selection in hybridization breeding.

小议汉语拼音教学的多样性

汉语拼音是小学生识字的基础,俗话说好比是识字的'拐杖'。新课标要求一位小学生要识3500多个汉字,不把汉语拼音学牢固,的确是一个问题。那么对于一位刚刚入学的孩子,怎样激发他们学习汉语拼音的兴趣,让他们能正确读、写汉语拼音字母、正确拼读音节,能独立根据拼音识字,这就需要教师在教学中多想办法,积累经验,实施汉语拼音教学。新课程标准明确指出'要遵循学生的身心发展规律和语言学习规律,选择教学策略。汉语拼音教学尽可能有趣味性,与学说普通话、识字教学相结合'。为此,我在汉语拼音教学中认为'易混字母区分记;特殊音节归类记;标调方法趣味记;难读音节实践记。'

Designing a SCAR molecular marker for monitoring Trichoderma cf. harzianum in experimental communities

Several species of the fungal genus Trichoderma establish biological interactions with various micro- and macro-organisms. Some of these interactions are relevant in ecological terms and in biotechnological applications, such as biocontrol, where Trichoderma could be considered as an invasive species that colonizes a recipient community. The success of this invasion depends on multiple factors, which can be assayed using experimental communities as study models. Therefore, the aim of this work is to develop a species-specific sequence-characterized amplified region （SCAR） marker to monitor the colonization and growth of T. cf. harzianum when it invades experi- mental communities. For this study, 16 randomly amplified polymorphic DNA （RAPD） primers of 10-mer were used to generate polymorphic patterns, one of which generated a band present only in strains of T. cf. harzianum. This band was cloned, sequenced, and five primers of 20-23 mer were designed. Primer pairs 2F2/2R2 and 2F2/2R3 successfully and specifically amplified fragments of 278 and 448 bp from the T. cf. harzianum BpT10a strain DNA, respectively. Both primer pairs were also tested against the DNA from 14 strains of T. cf. harzianum and several strains of different fungal genera as specificity controls. Only the DNA from the strains of T. cf. hat-zianum was successfully amplified. Moreover, primer pair 2F2/2R2 was assessed by quantitative real-time polymerase chain reaction （PCR） using fungal DNA mixtures and DNA extracted from fungal experimental communities as templates. T. cf. harzianum was detectable even when as few as 100 copies of the SCAR marker were available or even when its population represented only 0.1% of the whole community.