Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The e...Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The experiment revealed that the amplitude of transient outward potassium channel current was reduced.The maximum activated current densities of control group and exposure group were 163.62±20.68 pA/pF and 98.74±16.57 pA/pF(n=12,P<0.01) respectively.The static magnetic field exposure affected the activation and inactivation process of transient outward potassium channel current.Due to the magnetic field exposure,the half-activation voltage of the activation curves changed from 5.59±1.96 mV to 27.87±7.24 mV(n=12,P<0.05) ,and the slope factor changed from 19.43±2.11 mV to 25.87±4.22 mV(n=12,P<0.05) .The half-inactivation voltage of the inactivation curves also changed from-56.09±0.89 mV to-57.16±1.10 mV(n=12,P>0.05) and the slope factor of the inactivation curves from 8.69±0.80 mV to 10.87±1.02 mV(n=12,P<0.05) .The results show that the static magnetic field can change the characteristics of transient outward K+ channel,and affect the physiological functions of neurons.展开更多
Integrated with GIS and remote sensing(RS) technology,a systematic analysis and its methodology for human-settlements social environment has been introduced.This methodology has been called spatial trend field model(S...Integrated with GIS and remote sensing(RS) technology,a systematic analysis and its methodology for human-settlements social environment has been introduced.This methodology has been called spatial trend field model(STFM).STFM's application history in the field of human-settlements social environment has been discussed at first.Then,some index data models have been created through STFM,which include population density trend field,human activity strength trend field,city-town spatial density trend field,urbanization ratio trend field,road density trend field,GDP spatial density trend field and PER-GDP spatial density trend field.With all above-mentioned indexes as input data,through Iterative Self-Organizing Data Analysis Techniques Algorithm(ISODATA),this paper makes a verification study of Chongqing municipality.The result of the case study confirms that STFM methodology is credible and has high efficiency for regional human-settlements study.展开更多
基金Supported by National Natural Science Foundation of China(No. 60674111)
文摘Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The experiment revealed that the amplitude of transient outward potassium channel current was reduced.The maximum activated current densities of control group and exposure group were 163.62±20.68 pA/pF and 98.74±16.57 pA/pF(n=12,P<0.01) respectively.The static magnetic field exposure affected the activation and inactivation process of transient outward potassium channel current.Due to the magnetic field exposure,the half-activation voltage of the activation curves changed from 5.59±1.96 mV to 27.87±7.24 mV(n=12,P<0.05) ,and the slope factor changed from 19.43±2.11 mV to 25.87±4.22 mV(n=12,P<0.05) .The half-inactivation voltage of the inactivation curves also changed from-56.09±0.89 mV to-57.16±1.10 mV(n=12,P>0.05) and the slope factor of the inactivation curves from 8.69±0.80 mV to 10.87±1.02 mV(n=12,P<0.05) .The results show that the static magnetic field can change the characteristics of transient outward K+ channel,and affect the physiological functions of neurons.
基金supported by National 11th Five-Year Technology Support Program (Grant No 2008BAH31B06)National Natural Science Foundation of China (Grant No50738007)
文摘Integrated with GIS and remote sensing(RS) technology,a systematic analysis and its methodology for human-settlements social environment has been introduced.This methodology has been called spatial trend field model(STFM).STFM's application history in the field of human-settlements social environment has been discussed at first.Then,some index data models have been created through STFM,which include population density trend field,human activity strength trend field,city-town spatial density trend field,urbanization ratio trend field,road density trend field,GDP spatial density trend field and PER-GDP spatial density trend field.With all above-mentioned indexes as input data,through Iterative Self-Organizing Data Analysis Techniques Algorithm(ISODATA),this paper makes a verification study of Chongqing municipality.The result of the case study confirms that STFM methodology is credible and has high efficiency for regional human-settlements study.
