AIM:To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma. METHODS:Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the ...AIM:To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma. METHODS:Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the Bcl-xL gene was measured by immunohistochemistry method. The correlation of Bcl-xL expression with clinical index was evaluated. After human colon cancer cell line HT29 was transfected with Bcl-xL small interfering RNA (siRNA), the anchorage-independent growth of cancer cells was detected by colony formation in soft agar and invasion ability of cancer cells was determined by a transwell model. RESULTS:The Bcl-xL expression was higher in cancerous tissue samples than in normal tissue samples (38.78 ± 11.36 vs 0.89 ± 0.35, P < 0.001), and was associated with the pathological grade, lymphnode metastasis and Duke’s stage of colorectal carcinoma. Transfection with Bcl-xL siRNA inhibited the colony formation and invasion ability of human colon cancer cell line HT29 in vitro. CONCLUSION:Bcl-xL gene plays an important role in carcinogenesis of human colorectal carcinoma and is associated with malignant biological behaviors of human colorectal carcinoma.展开更多
AIM:To analyze the operative and perioperative factors associated with hepatectomy of benign hepatic lesions.METHODS:A total of 827 condecutive cases of benign hepatic lesion undergoing hepatectomy from January 1986 t...AIM:To analyze the operative and perioperative factors associated with hepatectomy of benign hepatic lesions.METHODS:A total of 827 condecutive cases of benign hepatic lesion undergoing hepatectomy from January 1986 to December 2005 in the Chinese PLA General Hospital were investigated retrospectively according to their medical documentation.RESULTS:The effect of operative and perioperative factors on the outcome of patients were analyzed.Of the 827 cases undergoing hepatectomy for more than 3 liver segments accounted for 22.1%,316(38.21%) required transfusion of blood products during operation.The average operating time was 220.59 ± 109.13 min,the average hospital stay after operation was 13.55 ± 9.38 d.Child-Pugh A accounted for 98.13%.The postoperative complication rate was 13.54% and the in-hospital mortality rate was 0.24%.Multivariate analysis showed that operating time(P = 0.004,OR = 1.003) and albumin value(P = 0.040,OR = 0.938) were the independent predictors of morbidity and indicated that operating time,blood transfusion,complication rate,and LOS had a trend to decrease.CONCLUSION:Hepatectomy for benign hepatic lesions can be performed safely with a low morbidity and mortality,provided that it is carried out with optimized perioperative management and an innovative surgical technique.展开更多
We report here how a heterotopic penetrating peptic ulcer progressed to cause small bowel obstruction in a pa- tient with multiple previous negative investigations. The clinical presentation, radiographic features and...We report here how a heterotopic penetrating peptic ulcer progressed to cause small bowel obstruction in a pa- tient with multiple previous negative investigations. The clinical presentation, radiographic features and patho- logical findings of this case are described, along with the salient lessons learnt. The added value of wireless cap- sule endoscopy （WCE） in such circumstances is debated.展开更多
In 1959, the concept of brain death （BD） or irreversible coma was described by Mollaret and Goulon （1959）. The first guideline （the Harvard criteria） for deciding BD was established in 1968 （Ad Hoc Committee of...In 1959, the concept of brain death （BD） or irreversible coma was described by Mollaret and Goulon （1959）. The first guideline （the Harvard criteria） for deciding BD was established in 1968 （Ad Hoc Committee of the Harvard Medical School, 1968）. This concept has been accepted worldwide although its fundamental meaning is not exactly globally uniform yet. Some countries （e.g., the US） view BD as ＂whole brain death＂, while others （e.g., the UK） as brain-stem death. The guidelines for the diagnosis of BD also differ among countries, even among hospitals in the same country.展开更多
A study was conducted to identify the factors related to diabetes mellitus among the diabetes patients at HUSM Kelantan. A total of 972 diabetes mellitus patients who had attended Hospital Universiti Sains Malaysia （...A study was conducted to identify the factors related to diabetes mellitus among the diabetes patients at HUSM Kelantan. A total of 972 diabetes mellitus patients who had attended Hospital Universiti Sains Malaysia （HUSM） from 2005 to 2006 were involved in the study. The significant findings include： （1） Mean age of diabetes patients under the study was 44.36 years old; （2） The average age of patients from control group and pre-diabetes group which came from the middle-age were 44.30 and 42.43 years old respectively. Patients from diabetes group had recorded the highest mean age of 53.24 years old which are the elderly; （3） There was a significant difference in the average blood sugar between patient age group of〈30 years old and patient age group of〉30 years old. However, there was no significant difference in the mean blood sugar between male and female patients; （4） There were significant associations between diabetes mellitus and gender, occupation and clinical symptoms such as tired, polyuria and polydipsia. However, there was no significant association between diabetes mellitus and polyphagia symptoms. The authors believed that the symptoms ofpolyuria and polydipsia were highly associated with the chances of the patients to get diabetes mellitus.展开更多
基金The Program of Science and Technology of Zhenjiang City, No. SH2005037, SH2006019
文摘AIM:To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma. METHODS:Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the Bcl-xL gene was measured by immunohistochemistry method. The correlation of Bcl-xL expression with clinical index was evaluated. After human colon cancer cell line HT29 was transfected with Bcl-xL small interfering RNA (siRNA), the anchorage-independent growth of cancer cells was detected by colony formation in soft agar and invasion ability of cancer cells was determined by a transwell model. RESULTS:The Bcl-xL expression was higher in cancerous tissue samples than in normal tissue samples (38.78 ± 11.36 vs 0.89 ± 0.35, P < 0.001), and was associated with the pathological grade, lymphnode metastasis and Duke’s stage of colorectal carcinoma. Transfection with Bcl-xL siRNA inhibited the colony formation and invasion ability of human colon cancer cell line HT29 in vitro. CONCLUSION:Bcl-xL gene plays an important role in carcinogenesis of human colorectal carcinoma and is associated with malignant biological behaviors of human colorectal carcinoma.
文摘AIM:To analyze the operative and perioperative factors associated with hepatectomy of benign hepatic lesions.METHODS:A total of 827 condecutive cases of benign hepatic lesion undergoing hepatectomy from January 1986 to December 2005 in the Chinese PLA General Hospital were investigated retrospectively according to their medical documentation.RESULTS:The effect of operative and perioperative factors on the outcome of patients were analyzed.Of the 827 cases undergoing hepatectomy for more than 3 liver segments accounted for 22.1%,316(38.21%) required transfusion of blood products during operation.The average operating time was 220.59 ± 109.13 min,the average hospital stay after operation was 13.55 ± 9.38 d.Child-Pugh A accounted for 98.13%.The postoperative complication rate was 13.54% and the in-hospital mortality rate was 0.24%.Multivariate analysis showed that operating time(P = 0.004,OR = 1.003) and albumin value(P = 0.040,OR = 0.938) were the independent predictors of morbidity and indicated that operating time,blood transfusion,complication rate,and LOS had a trend to decrease.CONCLUSION:Hepatectomy for benign hepatic lesions can be performed safely with a low morbidity and mortality,provided that it is carried out with optimized perioperative management and an innovative surgical technique.
文摘We report here how a heterotopic penetrating peptic ulcer progressed to cause small bowel obstruction in a pa- tient with multiple previous negative investigations. The clinical presentation, radiographic features and patho- logical findings of this case are described, along with the salient lessons learnt. The added value of wireless cap- sule endoscopy （WCE） in such circumstances is debated.
文摘In 1959, the concept of brain death （BD） or irreversible coma was described by Mollaret and Goulon （1959）. The first guideline （the Harvard criteria） for deciding BD was established in 1968 （Ad Hoc Committee of the Harvard Medical School, 1968）. This concept has been accepted worldwide although its fundamental meaning is not exactly globally uniform yet. Some countries （e.g., the US） view BD as ＂whole brain death＂, while others （e.g., the UK） as brain-stem death. The guidelines for the diagnosis of BD also differ among countries, even among hospitals in the same country.
文摘A study was conducted to identify the factors related to diabetes mellitus among the diabetes patients at HUSM Kelantan. A total of 972 diabetes mellitus patients who had attended Hospital Universiti Sains Malaysia （HUSM） from 2005 to 2006 were involved in the study. The significant findings include： （1） Mean age of diabetes patients under the study was 44.36 years old; （2） The average age of patients from control group and pre-diabetes group which came from the middle-age were 44.30 and 42.43 years old respectively. Patients from diabetes group had recorded the highest mean age of 53.24 years old which are the elderly; （3） There was a significant difference in the average blood sugar between patient age group of〈30 years old and patient age group of〉30 years old. However, there was no significant difference in the mean blood sugar between male and female patients; （4） There were significant associations between diabetes mellitus and gender, occupation and clinical symptoms such as tired, polyuria and polydipsia. However, there was no significant association between diabetes mellitus and polyphagia symptoms. The authors believed that the symptoms ofpolyuria and polydipsia were highly associated with the chances of the patients to get diabetes mellitus.
