According to the heating effect caused by interaction between matters,a series of experiments on the interaction between drugs and cells from human bodies,DNA and physiological saline have been carried out with a MS-8...According to the heating effect caused by interaction between matters,a series of experiments on the interaction between drugs and cells from human bodies,DNA and physiological saline have been carried out with a MS-80 standard Calvet microcalorimeter.The experiments include: (1) Thermokinetic studies of the effect of anticancer drugs [sodium norcantharidate (ASN),the bioac- tire materials (Sp.P and Sp.S) from algae etc.]on the cancer cells [Hela,human breast carcinoma (Bcap-37),human adenocarcinoma gastric cells (SGc-7901 and MCF-7) etc.] and the normal cells from human bodies [diploid fibroblasts from human fetal lung (2BS) etc.] at 310.15 K:(2) Studies of the in- tercalation binding of some alkaloidal drugs with the bioactivity to inhibit monoamine oxidase (harmalinc and harmine etc.) to call thymus DNA in neutral aqueous solution at 298.15 K:(3) Studies of the interaction between long acting drugs (long acting oral contraceptive-norgestrel etc.) and slow- releasing drug (Contac) and aqueous solution of 0.9% NaCI at 310.15 K.All the experimental results have given their characteristic thermograms and the interaction enthalpy changes.On the analysis of all the results,the authors put forward a method on application of microcalorimetric technique for screen- ing and examination of medicines.The principle of application and the experimental operation of this method have been expounded,and some results of the above experiments have been discussed.As one of the methods for screening and examining medicines,the microcalorimctric method has some distin- guished features and advantages over other methods.展开更多
AIM： To define the clinical characteristics, and to as- sess the management of colonoscopic complications at a local clinic. METHODS： A retrospective review of the medical re- cords was performed for the patients wi...AIM： To define the clinical characteristics, and to as- sess the management of colonoscopic complications at a local clinic. METHODS： A retrospective review of the medical re- cords was performed for the patients with iatrogenic colon perforations after endoscopy at a local clinic be- tween April 2006 and December 2010. Data obtained from a tertiary hospital in the same region were also analyzed. The underlying conditions, clinical presenta- tions, perforation locations, treatment types （operative or conservative） and outcome data for patients at the local clinic and the tertiary hospital were compared. RESULTS： A total of 10 826 colonoscopies, and 2625 therapeutic procedures were performed at a local clinic and 32 148 colonoscopies, and 7787 therapeutic proce-dures were performed at the tertiary hospital. The clinic had no perforations during diagnostic colonoscopy and 8 （0.3%） perforations were determined to be related to therapeutic procedures. The perforation rates in each therapeutic procedure were 0.06% （1/1609） in polyp- ectomy, 0.2% （2/885） in endoscopic mucosal resection （EMR）, and 3.8% （5/131） in endoscopic submucosal dis- section （ESD）. Perforation rates for ESD were significantly higher than those for polypectomy or EMR （P 〈 0.01）. All of these patients were treated conservatively. On the oth- er hand, three （0.01%） perforation cases were observed among the 24 361 diagnostic procedures performed, and these cases were treated with surgery in a tertiary hospi- tal. Six perforations occurred with therapeutic endoscopy （perforation rate, 0.08%; 1 per 1298 procedures）. Perfo- ration rates for specific procedure types were 0.02% （1 per 5500） for polypectomy, 0.17% （1 per 561） for EMR, 2.3% （1 per 43） for ESD in the tertiary hospital. There were no differences in the perforation rates for each therapeutic procedure between the clinic and the tertiary hospital. The incidence of iatrogenic perforation requiring surgical treatment was quite low in both the clinic and the tertiary hospital. No procedure-related mortalities occurred. Performing closure with endoscopic clipping reduced the C-reactive protein （CRP） titers. The mean maximum CRP titer was 2.9：1：1.6 mg/dL with clipping and 9.7 ＋ 6.2 mg/dL without clipping, respectively （P 〈 0.05）. An operation is indicated in the presence of a large perforation, and in the setting of generalized peritonitis or ongoing sepsis. Although we did not experience such case in the clinic, patients with large perforations should be immediately transferred to a tertiary hospital. Good relationships between local clinics and nearby tertiary hospitals should therefore be maintained. CONCLUSION： It was therefore found to be possible to perform endoscopic treatment at a local clinic when sufficient back up was available at a nearby tertiary hospital.展开更多
AIM: To evaluate the quality of gastric ulcer healing after different antiulcer treatment by endoscopic ultrasonography(EUS).METHODS: The patients were divided into three groups, and received lansoprazole, amoxicillin...AIM: To evaluate the quality of gastric ulcer healing after different antiulcer treatment by endoscopic ultrasonography(EUS).METHODS: The patients were divided into three groups, and received lansoprazole, amoxicillin and clarithromycinfor 1 wk. Then group A took lansoprazole combined with tepreton for 5 wk, group B took lansoprazole and group C took tepreton for 5 wk. Endoscopy and EUS were performed before and 6 wk after medication. RESULTS: There was no significant difference in cumulative healing rate to S stage between the groups (89%, 82%vs83%,P＞0.05). The rate of white scar formation was significantly higher in group A than in groups B and C (67%, 36%, 50%, P＜0.05). The average contraction rates of the width of ulcer crater, length of disrupted muscularis propria layer and hypoechoic area were higher in groupA than in groups B and C (0.792±0.090, 0.660±0.105 vs0.668±0.143, P＜0.05). The hypoechoic area disappeared in four cases of group A, one of group B and two of group C. The percentage of hypoechoic area disappearance was higher in group A than in the other two groups (44%, 9%vs 17%, P＜0.05). Gastric ulcer healing was better ingroup A.CONCLUSION: The combined administration of proton pump inhibitors and mucosal protective agent can improve gastric ulcer healing.展开更多
Objective To establish correlation models between various physical examination indexes and traditional Chinese medicine(TCM)constitutions,and explore their relationships based on the radial basis function(RBF)neural n...Objective To establish correlation models between various physical examination indexes and traditional Chinese medicine(TCM)constitutions,and explore their relationships based on the radial basis function(RBF)neural network.Methods The raw data of physical examination indexes and TMC constitutions of 650 subjects who underwent a physical examination were cleaned,classified and sorted,on the basis of which valid data were retrieved and categorized into a training dataset and a test dataset.Subsequently,the RBF neural network was applied to the valid samples in the training set to establish correlation models between various physical examination indexes and TCM constitutions.The accuracy and the error margin of the correlation model were then verified using the valid samples in the test set.Results Of all selected samples,the highest accuracy rates were 80% for the blood lipid index-TCM constitution model;100% for the renal function index-TCM constitution model;100% for the blood routine(male)index-TCM constitution model;88.8% for the blood routine(female)index-TCM constitution model;84.1%for the urine routine index-TCM constitution model;and 100% for the blood transfusion index-TCM constitution model.Conclusions The samples selected in this study suggested that there is a strong correlation between physical examination indexes and TCM constitutions,making it feasible to apply the established correlation models to TCM constitution identification.展开更多
文摘According to the heating effect caused by interaction between matters,a series of experiments on the interaction between drugs and cells from human bodies,DNA and physiological saline have been carried out with a MS-80 standard Calvet microcalorimeter.The experiments include: (1) Thermokinetic studies of the effect of anticancer drugs [sodium norcantharidate (ASN),the bioac- tire materials (Sp.P and Sp.S) from algae etc.]on the cancer cells [Hela,human breast carcinoma (Bcap-37),human adenocarcinoma gastric cells (SGc-7901 and MCF-7) etc.] and the normal cells from human bodies [diploid fibroblasts from human fetal lung (2BS) etc.] at 310.15 K:(2) Studies of the in- tercalation binding of some alkaloidal drugs with the bioactivity to inhibit monoamine oxidase (harmalinc and harmine etc.) to call thymus DNA in neutral aqueous solution at 298.15 K:(3) Studies of the interaction between long acting drugs (long acting oral contraceptive-norgestrel etc.) and slow- releasing drug (Contac) and aqueous solution of 0.9% NaCI at 310.15 K.All the experimental results have given their characteristic thermograms and the interaction enthalpy changes.On the analysis of all the results,the authors put forward a method on application of microcalorimetric technique for screen- ing and examination of medicines.The principle of application and the experimental operation of this method have been expounded,and some results of the above experiments have been discussed.As one of the methods for screening and examining medicines,the microcalorimctric method has some distin- guished features and advantages over other methods.
文摘AIM： To define the clinical characteristics, and to as- sess the management of colonoscopic complications at a local clinic. METHODS： A retrospective review of the medical re- cords was performed for the patients with iatrogenic colon perforations after endoscopy at a local clinic be- tween April 2006 and December 2010. Data obtained from a tertiary hospital in the same region were also analyzed. The underlying conditions, clinical presenta- tions, perforation locations, treatment types （operative or conservative） and outcome data for patients at the local clinic and the tertiary hospital were compared. RESULTS： A total of 10 826 colonoscopies, and 2625 therapeutic procedures were performed at a local clinic and 32 148 colonoscopies, and 7787 therapeutic proce-dures were performed at the tertiary hospital. The clinic had no perforations during diagnostic colonoscopy and 8 （0.3%） perforations were determined to be related to therapeutic procedures. The perforation rates in each therapeutic procedure were 0.06% （1/1609） in polyp- ectomy, 0.2% （2/885） in endoscopic mucosal resection （EMR）, and 3.8% （5/131） in endoscopic submucosal dis- section （ESD）. Perforation rates for ESD were significantly higher than those for polypectomy or EMR （P 〈 0.01）. All of these patients were treated conservatively. On the oth- er hand, three （0.01%） perforation cases were observed among the 24 361 diagnostic procedures performed, and these cases were treated with surgery in a tertiary hospi- tal. Six perforations occurred with therapeutic endoscopy （perforation rate, 0.08%; 1 per 1298 procedures）. Perfo- ration rates for specific procedure types were 0.02% （1 per 5500） for polypectomy, 0.17% （1 per 561） for EMR, 2.3% （1 per 43） for ESD in the tertiary hospital. There were no differences in the perforation rates for each therapeutic procedure between the clinic and the tertiary hospital. The incidence of iatrogenic perforation requiring surgical treatment was quite low in both the clinic and the tertiary hospital. No procedure-related mortalities occurred. Performing closure with endoscopic clipping reduced the C-reactive protein （CRP） titers. The mean maximum CRP titer was 2.9：1：1.6 mg/dL with clipping and 9.7 ＋ 6.2 mg/dL without clipping, respectively （P 〈 0.05）. An operation is indicated in the presence of a large perforation, and in the setting of generalized peritonitis or ongoing sepsis. Although we did not experience such case in the clinic, patients with large perforations should be immediately transferred to a tertiary hospital. Good relationships between local clinics and nearby tertiary hospitals should therefore be maintained. CONCLUSION： It was therefore found to be possible to perform endoscopic treatment at a local clinic when sufficient back up was available at a nearby tertiary hospital.
基金Supported by the Grant from the Eisai Human Health Care Company
文摘AIM: To evaluate the quality of gastric ulcer healing after different antiulcer treatment by endoscopic ultrasonography(EUS).METHODS: The patients were divided into three groups, and received lansoprazole, amoxicillin and clarithromycinfor 1 wk. Then group A took lansoprazole combined with tepreton for 5 wk, group B took lansoprazole and group C took tepreton for 5 wk. Endoscopy and EUS were performed before and 6 wk after medication. RESULTS: There was no significant difference in cumulative healing rate to S stage between the groups (89%, 82%vs83%,P＞0.05). The rate of white scar formation was significantly higher in group A than in groups B and C (67%, 36%, 50%, P＜0.05). The average contraction rates of the width of ulcer crater, length of disrupted muscularis propria layer and hypoechoic area were higher in groupA than in groups B and C (0.792±0.090, 0.660±0.105 vs0.668±0.143, P＜0.05). The hypoechoic area disappeared in four cases of group A, one of group B and two of group C. The percentage of hypoechoic area disappearance was higher in group A than in the other two groups (44%, 9%vs 17%, P＜0.05). Gastric ulcer healing was better ingroup A.CONCLUSION: The combined administration of proton pump inhibitors and mucosal protective agent can improve gastric ulcer healing.
基金the funding support from the National Key Research and Development Project of China(No.2018YFC1707606)National Natural Science Foundation of China(No.81904324)Youth Foundation of Sichuan Administration of Traditional Chinese Medicine(No.2016Q065).
文摘Objective To establish correlation models between various physical examination indexes and traditional Chinese medicine(TCM)constitutions,and explore their relationships based on the radial basis function(RBF)neural network.Methods The raw data of physical examination indexes and TMC constitutions of 650 subjects who underwent a physical examination were cleaned,classified and sorted,on the basis of which valid data were retrieved and categorized into a training dataset and a test dataset.Subsequently,the RBF neural network was applied to the valid samples in the training set to establish correlation models between various physical examination indexes and TCM constitutions.The accuracy and the error margin of the correlation model were then verified using the valid samples in the test set.Results Of all selected samples,the highest accuracy rates were 80% for the blood lipid index-TCM constitution model;100% for the renal function index-TCM constitution model;100% for the blood routine(male)index-TCM constitution model;88.8% for the blood routine(female)index-TCM constitution model;84.1%for the urine routine index-TCM constitution model;and 100% for the blood transfusion index-TCM constitution model.Conclusions The samples selected in this study suggested that there is a strong correlation between physical examination indexes and TCM constitutions,making it feasible to apply the established correlation models to TCM constitution identification.
