Effect of three suture lines on the proliferation and cell cycle of lung adenocarcinoma cell A549 in vitro

Objective： The interaction of cell and medical biomaterial is one of the significant factors to affect clinical application of medical biomaterial. This research is to investigate three of suture lines how to affect the proliferation and cell cycle of lung adenocarcinoma cell A549 in vitro. Methods： Three of suture lines were respectively cultivated with lung adenocarcinoma cell A549, after of 72 hours, we detected absorptions of each group by MTT method in order to reflect the proliferation of lung adenocarcinoma cell A549, and also examined percentage of G1 period cells and S period cells of each group by flow cytometry. Results： Different of suture lines had different effects on the proliferation and cell cycle of lung adenocarcinoma cell A549 （P 〈 0.05）. The effect of absorbent suture line was the strongest on the proliferation and cell cycle of lung arlenecarcinoma cell A549, the effect of chorda serica chirurgicalis was medium, and the effect of slide wire was poor. Different length of each suture line had different effects on the proliferation and cell cycle of lung adenocarcinoma cell A549 （P 〈 0.05）, Conclusion： Three of suture line materials have different effects on the proliferation and cell cycle of lung adenocarcinoma cell A549, with dose-effect relationship.

甲壳胺在临床医药学中的应用

甲壳胺(Chitosan)又名乙酰甲无胺、壳聚糖,可溶性甲壳质。C.A.登录号.[9012—76—4],国外商品名:“Flonac N”。它是在自然界广泛存在的甲壳质类(Chitin)最重要的生物之一。甲壳胺是甲壳质脱去N—乙酰的衍生物,化学结构为直链β—(1—4)—2—氨基—2—去氧—D—葡萄糖。分子量约12～59万,约185℃分解。甲壳质和甲壳胺在结构上的实际区别在脱乙酰的程度。甲壳胺的质量常以脱乙酰度、溶解度、溶液粘度、色泽、灰分等来衡量,它在临床医学和药学的研究及应用方面有着广泛的用途。