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Arbuscular mycorrhizal for-mation of crucifer leaf mus-tard induced by flavonoids apigenin and daidzein 被引量:2
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作者 DONG Changjin1,2 & ZHAO Bin1 1. State Key Laboratory of Agricultural Microbiology, Huazhong Agri- cultural University, Wuhan 430070, China 2. Department of Biology, Hubei Normal University, Huangshi 435002, China 《Chinese Science Bulletin》 SCIE EI CAS 2004年第12期1254-1261,共8页
Flavonoids from legume root secretion may probably act as signal molecules for expression of Rhizobial “nod” nodulation genes and AM fungal symbiotic gene. Leaf mustard is a non-mycorrhizal plant; it does not contai... Flavonoids from legume root secretion may probably act as signal molecules for expression of Rhizobial “nod” nodulation genes and AM fungal symbiotic gene. Leaf mustard is a non-mycorrhizal plant; it does not contain fla- vonoids and other signal molecules. AM fungi could not in- fect the roots of leaf mustard and form a symbiont in nature, when it was treated with flavonoids (apigenin or daidzein). The results of trypan blue staining showed that two kinds of AM fungi (G. intraradices and G. mosseae) successfully in- fected the roots of non-mycorrhizal plant leaf mustard. AM fungi grew towards and colonized the roots of leaf mustard, producing young spores and completing the course of life. AM fungi are the only one kind of fungi with ALP activity. The result of ALP staining has also proved that AM fungi infected successfully the roots of leaf mustard. AM fungi (G. intraradices and G. mosseae) that existed in the roots of non-mycorrhizal plant leaf mustard were probed by nested PCR and special molecular probes. The above-mentioned proof chains have fully proved that flavonoids induced AM fungi (G. intraradices and G. mosseae) to infect non-my- corrhizal plant and establish symbiotic relationship. 展开更多
关键词 非菌根植物 免疫 十字花科叶芥菜 芹甙元 大豆黄酮 类黄酮 灌木菌根真菌 嵌套PCR trypan蓝色染色
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