[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yel...[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the changes of chlorophyll content, activity and gene expression of caspase-like proteases in the tobacco leaves were studied. [Results] Compared with treatments of white, red and yellow film, blue and purple films delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage, and relatively lowered the activity and gene expression of caspase-like proteases during growth, development and senescence periods. [Conclusion] Different light qualities exhibited various effects on the growth, development and senescence of tobacco leaves, possibly by affecting the activity and gene expression of caspase-like proteases to some extent.展开更多
Sporadic rain events that occur during summer play an important role in the initiation of biological activity of semi-arid grasslands.To understand how ecosystem processes of a buffel grass(Cenchrus ciliaris L.)-domin...Sporadic rain events that occur during summer play an important role in the initiation of biological activity of semi-arid grasslands.To understand how ecosystem processes of a buffel grass(Cenchrus ciliaris L.)-dominated grassland respond to summer rain events,an LI 6 400 gas exchange system was used to measure the leaf gas exchange and plant canopy chambers were used to measure net ecosystem CO2exchange(NEE) and ecosystem respiration(Reco), which were made sequentially during periods before rain(dry) and after rain(wet). Gross ecosystem photosynthesis(GEP) was estimated from NEE and Reco fluxes, and light use efficiency parameters were estimated using a rectangular hyperbola model. Prior to the monsoon rain, grassland biomass was non-green and dry exhibiting positive NEE(carbon source) and low GEP values during which the soil water became increasingly scarce. An initial rain pulse(60 mm) increased the NEE from pre-monsoon levels to negative NEE(carbon gain) with markedly higher GEP and increased green biomass. The leaf photosynthesis and leaf stomatal conductance were also improved substantially. The maximum net CO2uptake(i.e.,negative NEE) was sustained in the subsequent period due to multiple rain events. As a result, the grassland acted as a net carbon sink for 20 d after first rain. With cessation of rain(drying cycle), net CO2 uptake was reduced to lower values. High sensitivity of this grassland to rain suggests that any decrease in precipitation in summer may likely affect the carbon sequestration of the semiarid ecosystem.展开更多
Forced response analysis of a rocket engine turbine blade was conducted by a decoupled fluid-structure interaction procedure.Aerodynamic forces on the rotor blade were obtained using 3D unsteady flow simulations. The ...Forced response analysis of a rocket engine turbine blade was conducted by a decoupled fluid-structure interaction procedure.Aerodynamic forces on the rotor blade were obtained using 3D unsteady flow simulations. The resulting aerodynamic forces were interpolated to the finite element(FE) model through surface effect elements prior to conducting forced response calculations.Effects of axial gap on aerodynamic forces were studied. In addition, influence of axial gap on the response of the shrouded blade was compared with that on the response of the unshrouded blade. Results demonstrated that as the axial gap increases,time-averaged pressure on the blade surface changes very little, while the pressure fluctuations decrease significantly. Pressure and aerodynamic forces on the blade surface display periodic variation, and the vane passing frequency component is dominant.Amplitudes of aerodynamic forces decrease with increasing axial gap. Restricted by the shroud, deformation and response of shrouded blade are much lower than those of the unshrouded blade. The response of unshrouded blade shows obvious beat vibration phenomenon, while the response of the shrouded blade does not have this characteristic because the shroud restrains multiple harmonics. Blade response in time domain was converted to frequency domain using fast Fourier transformation(FFT).Results revealed that the axial gap mainly affects the forced harmonic at the vane passing frequency, while the other two harmonics at natural frequency are hardly affected. Amplitudes of the unshrouded blade response decrease as the axial gap increases, while amplitudes of the shrouded blade response change very little in comparison.展开更多
基金Supported by National Natural Science Foundation of China(30460016)Science and Technology Plan from Yunnan Branch Office of China National Tobacco Corporation(2011YN03,2010YN03,07A01)~~
文摘[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the changes of chlorophyll content, activity and gene expression of caspase-like proteases in the tobacco leaves were studied. [Results] Compared with treatments of white, red and yellow film, blue and purple films delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage, and relatively lowered the activity and gene expression of caspase-like proteases during growth, development and senescence periods. [Conclusion] Different light qualities exhibited various effects on the growth, development and senescence of tobacco leaves, possibly by affecting the activity and gene expression of caspase-like proteases to some extent.
基金supported by Deutscher Akademischer Austausch Dienst(DAAD),Germanythe University of Bayreuth,Germany.the logistic support provided by Dr.Sudhakar SWAMY and technical staff from Madurai Kamaraj University,India
文摘Sporadic rain events that occur during summer play an important role in the initiation of biological activity of semi-arid grasslands.To understand how ecosystem processes of a buffel grass(Cenchrus ciliaris L.)-dominated grassland respond to summer rain events,an LI 6 400 gas exchange system was used to measure the leaf gas exchange and plant canopy chambers were used to measure net ecosystem CO2exchange(NEE) and ecosystem respiration(Reco), which were made sequentially during periods before rain(dry) and after rain(wet). Gross ecosystem photosynthesis(GEP) was estimated from NEE and Reco fluxes, and light use efficiency parameters were estimated using a rectangular hyperbola model. Prior to the monsoon rain, grassland biomass was non-green and dry exhibiting positive NEE(carbon source) and low GEP values during which the soil water became increasingly scarce. An initial rain pulse(60 mm) increased the NEE from pre-monsoon levels to negative NEE(carbon gain) with markedly higher GEP and increased green biomass. The leaf photosynthesis and leaf stomatal conductance were also improved substantially. The maximum net CO2uptake(i.e.,negative NEE) was sustained in the subsequent period due to multiple rain events. As a result, the grassland acted as a net carbon sink for 20 d after first rain. With cessation of rain(drying cycle), net CO2 uptake was reduced to lower values. High sensitivity of this grassland to rain suggests that any decrease in precipitation in summer may likely affect the carbon sequestration of the semiarid ecosystem.
文摘Forced response analysis of a rocket engine turbine blade was conducted by a decoupled fluid-structure interaction procedure.Aerodynamic forces on the rotor blade were obtained using 3D unsteady flow simulations. The resulting aerodynamic forces were interpolated to the finite element(FE) model through surface effect elements prior to conducting forced response calculations.Effects of axial gap on aerodynamic forces were studied. In addition, influence of axial gap on the response of the shrouded blade was compared with that on the response of the unshrouded blade. Results demonstrated that as the axial gap increases,time-averaged pressure on the blade surface changes very little, while the pressure fluctuations decrease significantly. Pressure and aerodynamic forces on the blade surface display periodic variation, and the vane passing frequency component is dominant.Amplitudes of aerodynamic forces decrease with increasing axial gap. Restricted by the shroud, deformation and response of shrouded blade are much lower than those of the unshrouded blade. The response of unshrouded blade shows obvious beat vibration phenomenon, while the response of the shrouded blade does not have this characteristic because the shroud restrains multiple harmonics. Blade response in time domain was converted to frequency domain using fast Fourier transformation(FFT).Results revealed that the axial gap mainly affects the forced harmonic at the vane passing frequency, while the other two harmonics at natural frequency are hardly affected. Amplitudes of the unshrouded blade response decrease as the axial gap increases, while amplitudes of the shrouded blade response change very little in comparison.
