增建半幅高速公路长大隧道火灾特性CFD分析

近年来,随着我国公路隧道的快速发展,其中相当数量的隧道需由低等级公路隧道改扩建成高等级公路隧道。文章针对长大增建半幅公路公路隧道,采用CFD分析方法,借用FDS火灾模拟软件,建立不同隧道火灾工况,研究得到了不同通风风速条件下主隧道内烟雾、温度及能见度分布规律并确定了其临界风速,同时研究得到了临界通风条件下、不同坡度条件下横通道内温度及能见度的分布规律。

观测到的半度增温对高温热浪的影响

利用1961—2017年全国逐日平均温度、最高温度和最低温度数据,研究半度增温对我国极端温度和高温热浪的影响。结果表明,在半度增温(1998—2017年与1970—1989年)下,我国大部分地区平均温度增幅超过0.5℃,全国大部分地区的白天最高温和夜晚最高温都明显增加,且夜间最高温度较白天最高温度增长速度更快,空间上存在较为一致的变化特征,我国北方地区极端温度的增幅大于南方地区,其中增加的大值区主要位于西北的东部、华北及东部沿海部分地区。

煤绒菌(Fuligo sp.)DNA提取方法的研究及用于扩增EF1A基因片段的引物设计

采用氯化苄法提取煤绒菌的基因组DNA,并成功获得PCR产物。同时采用Primer 5.0软件设计得到延长因子EF1A特异性引物EF243、ER824和ER1001,用这些引物对煤绒菌的延长因子EF1A基因进行半巢式PCR扩增获得成功。

Construction of Fusion Expression Vector of α-galactosidase-EGFP in Cucumber

[Objective] The research aimed to construct the fusion protein expression vector of α-galactosidase-EGFP （enhanced green fluorescent protein） in cucumber controlled by CaMV35S promoter.[Method] CaMV35S promoter sequence and the coding region of EGFP were amplified by polymerase chain reactions （PCR） with vector pCambia 1303 as the template.Using reverse transcript PCR technology,with total RNAs of cucumber as template,the coding region of acid α-galactosidase Ⅰ in cucumber was amplified.The above three fragments were inserted into the multiple cloning sites of expression vector pCambia 1381c.The fusion expression vector of α-galactosidase-EGFP located at the C-terminal of the target genes was constructed.[Result] After enzyme digestion and sequencing,the fusion expression of α-galactosidase-EGFP in cucumber was constructed successfully.[Conclusion] The research laid the experimental basis for further study on the subcellular localization of α-galactosidase in cucumber.

端粒酶活性检测对良恶性腹水鉴别诊断的价值

目的 通过检测腹水脱落细胞端粒酶活性 ,探讨端粒酶对良恶性腹水鉴别诊断的价值。方法 用半定量端粒重复序列扩增法 (TRAP) ,检测 38例恶性腹水患者 (恶性腹水组 )和 2 2例良性腹水患者 (良性腹水组 )腹水中脱落细胞端粒酶活性。结果 肝硬化、结核性腹膜炎患者腹水中脱落细胞未检出端粒酶 ,而癌性腹水94 .7%端粒酶阳性。所有细胞学检查阳性的恶性腹水中端粒酶均为阳性 ,而 3例细胞学检查阴性但端粒酶阳性的恶性腹水 ,复查细胞学检查 ,其中 1例发现了恶性瘤细胞。结论 癌性腹水端粒酶阳性。端粒酶活性检测对良恶性腹水有重要鉴别诊断价值 。

Gain Switch of an AlGaInP Red Light Semiconductor Laser Diode

We solve the single mode coupled rate equations by computer,simulate the behavior of a gain switch of an AlGaInP red light semiconductor laser diode,and find the characteristic of FWHM of pulses changing with the amplitude of modulation signal, the bias current, and the modulated frequency. On this basis, we conduct experiments. The experiment results accord with the simulations well.

Incremental semi-supervised learning for intelligent seismic facies identification

Intelligent seismic facies identification based on deep learning can alleviate the time-consuming and labor-intensive problem of manual interpretation,which has been widely applied.Supervised learning can realize facies identification with high efficiency and accuracy;however,it depends on the usage of a large amount of well-labeled data.To solve this issue,we propose herein an incremental semi-supervised method for intelligent facies identification.Our method considers the continuity of the lateral variation of strata and uses cosine similarity to quantify the similarity of the seismic data feature domain.The maximum-diff erence sample in the neighborhood of the currently used training data is then found to reasonably expand the training sets.This process continuously increases the amount of training data and learns its distribution.We integrate old knowledge while absorbing new ones to realize incremental semi-supervised learning and achieve the purpose of evolving the network models.In this work,accuracy and confusion matrix are employed to jointly control the predicted results of the model from both overall and partial aspects.The obtained values are then applied to a three-dimensional(3D)real dataset and used to quantitatively evaluate the results.Using unlabeled data,our proposed method acquires more accurate and stable testing results compared to conventional supervised learning algorithms that only use well-labeled data.A considerable improvement for small-sample categories is also observed.Using less than 1%of the training data,the proposed method can achieve an average accuracy of over 95%on the 3D dataset.In contrast,the conventional supervised learning algorithm achieved only approximately 85%.

A novel derivative of xanomeline improved memory function in aged mice

Objective To characterize the function of a new xanomeline-derived M 1 agonist, 3-[3-（3-florophenyl-2-propyn- 1- ylthio）-1,2,5-thiadiazol-4-yl]-1,2,5,6- tetrahydro-1-methylpyridine Oxalate （EUK1001）, the acute toxicity and the effects on synaptic plasticity and cognition of EUK1001 were evaluated. Methods To examine the median lethal dose （LD50） of EUK1001, a wide dose range of EUK1001 was administered by p.o. and i.p. in aged mice. Furthermore, novel object recognition task and in vitro electrophysiological technique were utilized to investigate the effects of EUK1001 on recognition memory and hippocampal synaptic plasticity in aged mice. Results EUK1001 exhibited lower toxicity than xanomeline, and improved the performance of aged mice in the novel object recognition test. In addition, bath application of 1 μmol/L EUK1001 directly induced long-term potentiation in the hippocampus slices. Conclusion We conclude that EUK1001 can improve the agerelated cognitive deficits.

求解一般l_(1)趋势过滤问题的原始对偶内点法

针对一般l_(1)趋势过滤问题提出一种原始对偶内点法,首先给出原始对偶内点法的算法框架,并对原始对偶内点法进行收敛性分析和算法复杂度分析.最后,将提出的算法和目前流行的半光滑牛顿增广拉格朗日方法和交替方向乘子法进行对比.实验结果表明:当模型中的参数变化时,原始对偶内点法更加高效和稳健.

A Fixed Point Theorem for Multi-valued Composite Increasing Operators

In this paper,we give a fixed point theorem for multi-valued composite increasing operators, which generalizes the theorem of the paper [1] under not only multi-valued mappings but also single-valued mappings. And we generalizes the corresponding results of [2]-[5].

Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis

The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability.

Hybrid WDM/TDM PON system employing an all-optical wavelength converter

A new hybrid WDM/TDM passive optical network （PON） implemented by using all-optical wavelength converters （AOWCs） is proposed. The AOWCs are based on the cross-gain modulation （XGM） effect of the semiconductor optical amplifier （SOA）. Moreover, the feasibility of this sys- tem is experimentally demonstrated by evaluating the impacts of the optical wavelength conversion, time domain waveforms, eye diagrams and bit-error-rate （BER） in AOWC. The results show that the proposal will be a promising solution for the next generation access networks.

半序度量空间中公共二元随机重合点定理及其应用

在完备可分的半序度量空间中,引入了随机映射对(F,G)关于g随机半序弱增以及(F,G)随机半序弱增的定义,研究了在满足一定非线性压缩条件下的随机映射列F_k:Ω×X×X→X,k=1,2…,g:Ω×X→X和h:Ω×X→X的公共二元随机重合点与公共二元随机不动点问题,所得结果推广了已有文献中的一些不动点定理.

维吾尔族与阿拉伯木卡姆音乐中同名调之结构比较

中国新疆维吾尔族木卡姆与阿拉伯地区木卡姆之间有深远悠久的历史文化联系,关于这一点,已有很多讨论与研究,但缺少从音乐结构方面的比较论证研究。通过实证比较得出的结论:在这两个地区的木卡姆音乐中,同名调并非同结构。由此可提出一个需要进一步讨论的问题:这种音乐结构上并无共同内核的乐调却有着共同的调名,其原因是什么?

Observations of gravity wave activity during stratospheric sudden warmings in the Northern Hemisphere

Arctic stratospheric sudden warming(SSW) with polar vortex displacement and splitting occurred in 2008 and 2009, respectively. The temperature profiles derived from the Constellation Observing System for Meteorology, Ionosphere and Climate(COSMIC) along with NCEP/NCAR reanalysis are used to investigate the gravity wave(GW) variations during these SSW events. Some phenomena are characterized and possible mechanisms are discussed. The GW variations during both types of SSW events shared some similarities. During the warming events, enhanced zonal mean GW amplitudes are detected. The enhancements of GW amplitudes first appeared at higher altitudes and then progressed downward during the major warming events. The downward progressions correspond well with the height variations of the zonal wind reversal, and the enhancements seem to occur at the same time with the wind reversal. The spatial variations of GWs showed evident correlation to the polar vortex. Generally, GWs were enhanced at the edge of the polar vortex where the background wind was strong, whereas the areas with the largest GW amplitudes did not always correspond with those with the strongest background wind. The differences during the two types of SSW are also noticeable. In the 2008 major event, the magnitudes of the zonal mean GW enhancements during the downward progression were relatively stable, whereas in the 2009 major event, a clear reduction was detected as the GW enhancement was progressing downward. Also, the recovery time of the polar vortex was longer in 2009. These differences indicate that different types of SSWs may have different impacts on the GW.

Non-Nehari manifold method for asymptotically periodic Schrodinger equations

We consider the semilinear Schrdinger equation-△u + V(x)u = f(x, u), x ∈ RN,u ∈ H 1(RN),where f is a superlinear, subcritical nonlinearity. We mainly study the case where V(x) = V0(x) + V1(x),V0∈ C(RN), V0(x) is 1-periodic in each of x1, x2,..., x N and sup[σ(-△ + V0) ∩(-∞, 0)] < 0 < inf[σ(-△ +V0)∩(0, ∞)], V1∈ C(RN) and lim|x|→∞V1(x) = 0. Inspired by previous work of Li et al.(2006), Pankov(2005)and Szulkin and Weth(2009), we develop a more direct approach to generalize the main result of Szulkin and Weth(2009) by removing the "strictly increasing" condition in the Nehari type assumption on f(x, t)/|t|. Unlike the Nahari manifold method, the main idea of our approach lies on finding a minimizing Cerami sequence for the energy functional outside the Nehari-Pankov manifold N0 by using the diagonal method.

Least energy solutions for semilinear Schrdinger equation with electromagnetic fields and critical growth

We study a class of semilinear SchrSdinger equation with electromagnetic fields and the nonlinearity term involving critical growth. We assume that the potential of the equation includes a parameter A and can be negative in some domain. Moreover, the potential behaves like potential well when the parameter A is large. Using variational methods combining Nehari methods, we prove that the equation has a least energy solution which, as the parameter A becomes large, localized near the bottom of the potential well. Our result is an extension of the corresponding result for the SchrSdinger equation which involves critical growth but does not involve electromagnetic fields.

AN OVERVIEW OF THE DEVELOPMENT OF LOW GAIN FEEDBACK AND LOW-AND-HIGH GAIN FEEDBACK

Low gain feedback refers to certain families of stabilizing state feedback gains that are pararaeterized in a scalar and go to zero as the scalar decreases to zero. Low gain feedback was initially proposed to achieve semi-global stabilization of linear systems subject to input saturation. It was then combined with high gain feedback in different ways for solving various control problems. The resulting feedback laws are referred to as low-and-high gain feedback. Since the introduction of low gain feedback in the context of semi-global stabilization of linear systems subject to input saturation, there has been effort to develop alternative methods for low gain design, to characterize key features of low gain feedback, and to explore new applications of the low gain and low-and-high gain feedback. This paper reviews the developments in low gain and low-and-high gain feedback designs.

超声造影引导下粗针活检联合XpertMTB/RIF检测在周围型肺结核诊断中的应用价值

目的:评估超声造影引导下粗针活检联合半巢式全自动实时荧光定量核酸扩增检测技术(XpertMTB/RIF)在周围型肺结核诊断中的应用价值。方法:回顾性分析2016年6月至2018年6月浙江省中西医结合医院结核科疑似周围型肺结核的71例住院患者,所有患者肺部肿块均经超声造影引导下穿刺活检,穿刺组织标本分别进行常规病理检查、XpertMTB/RIF检测及结核分枝杆菌(MTB)培养,以“肺结核诊断和治疗指南”为诊断金标准,比较3种检测方法诊断周围型肺结核的敏感度、特异度、阳性预测值、阴性预测值及准确性。并计算受试者工作特征(ROC)曲线下面积(AUC),采用t检验比较3种诊断方法的诊断效能差异。以药敏试验为诊断结核标准,评估XpertMTB/RIF方法检验利福平耐药的准确性。结果:71例患者诊断为周围型肺结核40例(结核组),非周围型肺结核患者31例(非结核组)。非结核组包括肺部恶性肿瘤10例,肺部良性肿瘤1例,间质性肺炎14例,胞内分枝杆菌肺炎3例,真菌性肺炎3例。常规病理、XpertMTB/RIF检测及MTB培养3种检测方法的诊断敏感度分别为80.0%、92.5%、12.5%,特异度分别为90.3%、100%、100%,阳性预测值分别为91.4%、100%、100%,阴性预测值分别为77.8%、91.2%、47.0%,准确性分别为84.5%、95.8%、50.7%。XpertMTB/RIF的诊断敏感度高于常规病理检查及MTB培养,差异具有统计学意义(χ2=33.632、5.976,P均<0.05)。ROC曲线分析显示常规病理、XpertMTB/RIF检测及MTB培养3种检查方法AUC分别为0.814、0.963、0.563,XpertMTB/RIF的诊断效能优于常规病理检查及MTB培养,AUC比较,差异具有统计学意义(t=7.579、2.303,P均<0.05)。结论:超声造影引导下粗针活检联合XpertMTB/RIF检测技术能快速、准确地对周围型肺结核进行诊断,相对于常规病理检查、MTB培养具有较高的诊断效能。

All-optical ultra-wideband doublet signal source based on the cross-gain modulation in a semiconductor optical amplifier

We propose a novel scheme to generate the ultra-wideband (UWB) doublet signal pulse based on the cross-gain modulation (XGM) in a semiconductor optical amplifier (SOA). In the scheme, only an optical source and an SOA are needed. As there is only one wavelength included in the output doublet signal pulse, no time difference between the upper and down pulses is introduced during the transmission process. By using the software of Optisystem 7.0, the impacts of the optical power, the SOA current, the wavelength and the input signal pulse width on the generated doublet pulse are simulated and tudied numerically. The results show that when the pulse width of the input signal pulse is larger, the output signal pulse is better, and is insensitive to the change of wavelength. In addition, the ultra-wideband positive and negative monocycles can be generated by choosing suitable optical source power and SOA current.