[Objective] The aim was to clone ISA1 from Oryza sativa and analyze its expression situation in different tissues and different endosperm filling stage.[Method] With japonica rice cultivar nipponbare as test material,...[Objective] The aim was to clone ISA1 from Oryza sativa and analyze its expression situation in different tissues and different endosperm filling stage.[Method] With japonica rice cultivar nipponbare as test material,the expression patterns of ISA1 in different tissues and different endosperm filling stage was analyzed by using semi-quantitative RT-PCR technique.[Result]The full length open reading fragments of ISA1 encoded 811 amino acid residues.The homologous alignment and phylogenetic analysis showed th...展开更多
基金Supported by Zhejiang Provincial Natural Science Foundation(Y3090617 and Y304463)~~
文摘[Objective] The aim was to clone ISA1 from Oryza sativa and analyze its expression situation in different tissues and different endosperm filling stage.[Method] With japonica rice cultivar nipponbare as test material,the expression patterns of ISA1 in different tissues and different endosperm filling stage was analyzed by using semi-quantitative RT-PCR technique.[Result]The full length open reading fragments of ISA1 encoded 811 amino acid residues.The homologous alignment and phylogenetic analysis showed th...