【目的】将抗逆相关转录因子ABP9基因导入大豆(Glycine max L.)基因组,并对转化系统条件进行探索,为提高大豆遗传转化效率提供依据。【方法】以农杆菌介导的半种法大豆遗传转化系统为基础,对目的基因进行转化;以健康外植体获得率、抗性...【目的】将抗逆相关转录因子ABP9基因导入大豆(Glycine max L.)基因组,并对转化系统条件进行探索,为提高大豆遗传转化效率提供依据。【方法】以农杆菌介导的半种法大豆遗传转化系统为基础,对目的基因进行转化;以健康外植体获得率、抗性丛生芽获得率和抗性芽伸长比率为指标,对转化系统条件进行优化。【结果】共培养时保证充足的光照和氧气,外植体生长情况最好;在共培养或诱导丛生芽培养基中加入600mg·L-1的L-半胱氨酸可以使抗性丛生芽获得率由22.1%提高至32.6%;采用4种植物激素进行正交试验,诱导抗性芽伸长比率最高的激素配比为:6-BA为1.5mg·L-1,GA3为0.75mg·L-1,IAA为0.1mg·L-1,ZT为1.0mg·L-1。【结论】利用优化的方法进行遗传转化研究已获得转基因再生植株,经PCR和DNA测序等分子检测,证明目的基因ABP9已导入并整合到大豆基因组中;转化率为1.33%。展开更多
Field experiments were conducted at Dabagi Farm of the Usmanu Danfodiyo University, Sokoto （13~I＇N and 5~15＇ E）, in the Semi-arid of Nigeria, during 2007 and 2008 rainy seasons to determine the effect of sowing me...Field experiments were conducted at Dabagi Farm of the Usmanu Danfodiyo University, Sokoto （13~I＇N and 5~15＇ E）, in the Semi-arid of Nigeria, during 2007 and 2008 rainy seasons to determine the effect of sowing methods on growth and fodder yield of Stylosanthes hamata cv. Verano. Three sowing methods （dibbling, broadcasting and drilling） were investigated. The experimental design adopted for the study was the randomized complete block design （RCBD） replicated three times. The parameters monitored included plant height （cm）, leaf length （cm）, leaf width （cm）, number of leaves, canopy spread （cm）, stand count and herbage yield. Samples were randomly taken and marked for the measurement of the parameters at 2, 4, 6, 8, 10, 12 and 14 weeks after sowing （WAS）. Herbage yield （kg hal） was determined at the end of the 14th week. Result of the study revealed that the sowing methods had significant effect （P 〈 0.05） on growth parameters at 2 to 14 WAS in 2007, 2008 and in their combined analysis. Dry matter yield differed （P 〈 0.05） significantly. Dibbling method produced plants that were taller, longer, with wider canopy spread and leaves as well as more leaves and stands （P 〈 0.05）. The year 2007 produced taller plants, longer, wider canopy spread and leaves as well as more leaves and stands （P 〈 0.05） in all the weeks compared to the year 2008. It was therefore concluded from the results of this study that dibbling method of sowing was the best for Stylosanthes hamata cv. Verano production at 50 ~ 50 cm inter and intra-row spacing in the semi-arid of Sokoto agro-ecology in northwestern Nigeria, presumably due to low competition for scarce moisture and creation of more space in this sowing method.展开更多
文摘【目的】将抗逆相关转录因子ABP9基因导入大豆(Glycine max L.)基因组,并对转化系统条件进行探索,为提高大豆遗传转化效率提供依据。【方法】以农杆菌介导的半种法大豆遗传转化系统为基础,对目的基因进行转化;以健康外植体获得率、抗性丛生芽获得率和抗性芽伸长比率为指标,对转化系统条件进行优化。【结果】共培养时保证充足的光照和氧气,外植体生长情况最好;在共培养或诱导丛生芽培养基中加入600mg·L-1的L-半胱氨酸可以使抗性丛生芽获得率由22.1%提高至32.6%;采用4种植物激素进行正交试验,诱导抗性芽伸长比率最高的激素配比为:6-BA为1.5mg·L-1,GA3为0.75mg·L-1,IAA为0.1mg·L-1,ZT为1.0mg·L-1。【结论】利用优化的方法进行遗传转化研究已获得转基因再生植株,经PCR和DNA测序等分子检测,证明目的基因ABP9已导入并整合到大豆基因组中;转化率为1.33%。
文摘Field experiments were conducted at Dabagi Farm of the Usmanu Danfodiyo University, Sokoto （13~I＇N and 5~15＇ E）, in the Semi-arid of Nigeria, during 2007 and 2008 rainy seasons to determine the effect of sowing methods on growth and fodder yield of Stylosanthes hamata cv. Verano. Three sowing methods （dibbling, broadcasting and drilling） were investigated. The experimental design adopted for the study was the randomized complete block design （RCBD） replicated three times. The parameters monitored included plant height （cm）, leaf length （cm）, leaf width （cm）, number of leaves, canopy spread （cm）, stand count and herbage yield. Samples were randomly taken and marked for the measurement of the parameters at 2, 4, 6, 8, 10, 12 and 14 weeks after sowing （WAS）. Herbage yield （kg hal） was determined at the end of the 14th week. Result of the study revealed that the sowing methods had significant effect （P 〈 0.05） on growth parameters at 2 to 14 WAS in 2007, 2008 and in their combined analysis. Dry matter yield differed （P 〈 0.05） significantly. Dibbling method produced plants that were taller, longer, with wider canopy spread and leaves as well as more leaves and stands （P 〈 0.05）. The year 2007 produced taller plants, longer, wider canopy spread and leaves as well as more leaves and stands （P 〈 0.05） in all the weeks compared to the year 2008. It was therefore concluded from the results of this study that dibbling method of sowing was the best for Stylosanthes hamata cv. Verano production at 50 ~ 50 cm inter and intra-row spacing in the semi-arid of Sokoto agro-ecology in northwestern Nigeria, presumably due to low competition for scarce moisture and creation of more space in this sowing method.
