华东片区不同于西南山区,铁路沿线地区经济发达、地物十分复杂,高压线、油气管线纵横交错、分布密集,水系发育、交织如网。结合华东片区高速铁路建设特点,以实际工程项目为例,依据多目标综合评价体系的构建原则建立环境选线方案优选的...华东片区不同于西南山区,铁路沿线地区经济发达、地物十分复杂,高压线、油气管线纵横交错、分布密集,水系发育、交织如网。结合华东片区高速铁路建设特点,以实际工程项目为例,依据多目标综合评价体系的构建原则建立环境选线方案优选的综合评价体系,并采用层次分析法确定其指标权重;将灰色关联法和TOPSIS法(Technique for Order Preference by Similarity to Ideal Solution)两种方法相结合,建立灰色关联改进的TOPSIS法的方案优选综合评价模型,并验证其可行性和合理性。展开更多
AIM： TO determine the genotype distribution of hepatitis B virus （HBV） with a newly oligonucleotide chip assay among the HBV carriers in Eastern China. METHODS： An assay using oligonucleotide chip was developed fo...AIM： TO determine the genotype distribution of hepatitis B virus （HBV） with a newly oligonucleotide chip assay among the HBV carriers in Eastern China. METHODS： An assay using oligonucleotide chip was developed for detection of HBV genotypes in serum samples from HBV DNA-positive patients in Eastern China. This method is based on the principle of reverse hybridization with Cy5-labeled amplicons hybridizing to type-specific oligonucleotide probes that are immobilized on slides. The results of 80 randomly chosen sera were confirmed by direct sequencing. RESULTS： HBV genotype B, C and mixed genotype were detected in 400 serum samples, accounting for 8.3% （n = 33）, 83.2% （n = 333）, and 8.5% （n = 34）, respectively. The evaluation of the oligonucleotide assay showed 100% concordance with the amplicon phylogenetic analysis except 9 mixed genotype infections undetected by sequencing. CONCLUSION： The study indicates that HBV genotype C and B prevail in the Eastern China. It is suggested that the oligonucleotide chip is a reliable and convenient tool for the detection of HBV genotyping.展开更多
文摘华东片区不同于西南山区,铁路沿线地区经济发达、地物十分复杂,高压线、油气管线纵横交错、分布密集,水系发育、交织如网。结合华东片区高速铁路建设特点,以实际工程项目为例,依据多目标综合评价体系的构建原则建立环境选线方案优选的综合评价体系,并采用层次分析法确定其指标权重;将灰色关联法和TOPSIS法(Technique for Order Preference by Similarity to Ideal Solution)两种方法相结合,建立灰色关联改进的TOPSIS法的方案优选综合评价模型,并验证其可行性和合理性。
文摘AIM： TO determine the genotype distribution of hepatitis B virus （HBV） with a newly oligonucleotide chip assay among the HBV carriers in Eastern China. METHODS： An assay using oligonucleotide chip was developed for detection of HBV genotypes in serum samples from HBV DNA-positive patients in Eastern China. This method is based on the principle of reverse hybridization with Cy5-labeled amplicons hybridizing to type-specific oligonucleotide probes that are immobilized on slides. The results of 80 randomly chosen sera were confirmed by direct sequencing. RESULTS： HBV genotype B, C and mixed genotype were detected in 400 serum samples, accounting for 8.3% （n = 33）, 83.2% （n = 333）, and 8.5% （n = 34）, respectively. The evaluation of the oligonucleotide assay showed 100% concordance with the amplicon phylogenetic analysis except 9 mixed genotype infections undetected by sequencing. CONCLUSION： The study indicates that HBV genotype C and B prevail in the Eastern China. It is suggested that the oligonucleotide chip is a reliable and convenient tool for the detection of HBV genotyping.
