[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were des...[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were designed based on EST sequences at 5’ region of Syntaxin, and cDNA obtained through reverse reaction was taken as the template. Sequences of Syntaxin gene at 3’ region were obtained through two rounds of PCR amplifications. [Result] DNA fragments （1 096 bp） were obtained. For LsSyntaxin, open reading frame （ORF） was 816 bp and the encoded amino acids were 271. The relative molecular weight of Syntaxin was 30 254.3 Da and isoelectric point in theory was 5.55. [Conclusion] Syntaxin genes from Limonium sinense Kuntze were cloned. The research laid foundation for the study on Syntaxin gene function in Limonium sinense Kuntze and salt-secreted process.展开更多
[Objective] This study aimed to observe and explore the behavior of Procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experime...[Objective] This study aimed to observe and explore the behavior of Procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experiment in a relatively small polyculture system. The agonistic behavior of P. clarkii and E. sinensis in the first 30 min of the initial encounter and the fighting results were observed and recorded; the approach-avoidance behavior of P. clarkii to bigger E. sinensis in a relatively large space （the ＂Y＂ maze） was observed and recorded. [Result] The re- sults showed that the times of first aggressions and approaches and the total times of active aggressions and approaches of P. clarkii in the initial period of polyculture experiment were all significantly more than E. sinensis （/：＇〈0.05）; after 30 d of poly- culture, P. clarkfi died in 30% of the groups while E. sinensis died in only 10% of the groups, and they coexisted in 60% of the groups. In the experiment of ap- proach-avoidance behavior of P. clarkii to bigger E. sinensis, with the increase of experiment times, the tendency of approach and avoidance of P. clarkii to the con- trol and E. sinensis became control-prefer （P〈0.05） from insignificantly different （P〉 0.05）, which indicated that the recognition ability of P. clarkii for E. sinensis was sig- nificantly improved, and the selection behavior of E. sinensis was obviously inhibited. [Conclusion] Presumably, polyculture of crayfishes and crabs can achieve better effi- ciency when under appropriate habitat conditions.展开更多
During 2009 and 2010, 23 night-time mosquito captures were made at Kanci obora in south-eastern Moravia, Czech Republic. It was used in the Centers for Disease Control and Prevention （CDCP） miniature light traps wit...During 2009 and 2010, 23 night-time mosquito captures were made at Kanci obora in south-eastern Moravia, Czech Republic. It was used in the Centers for Disease Control and Prevention （CDCP） miniature light traps with CO2 （dry ice） and baited lard-can traps in which sentinel animals were replaced with a container filled with CO2 （dry ice）. In the observed period, a total of 31,882 female mosquitoes were captured by CDC miniature light traps with CO2. Lard-can traps baited with CO2 captured 995 females under the same conditions, which is just 3.12% of the quantity from the CDC traps. At the same time, there were significant differences in the proportional captures of various species. Compared to CDC miniature light traps, baited lard-can traps much more often captured Aedes cinereus （16.58% of total versus 1.93% in CDC traps）, Culex modestus （15.48% versus 4.62%）, and Ae. rossicus （6.13% versus 2.67%）. On the other hand, capture of female Ae. vexans was proportionally much lower （15.38% versus 36.41%）. Capture of Cx. pipiens was more or less the same 14.77% （miniature light traps） and 15.76% （baited lard-can traps）. The occurrence of the calamity species Ae. sticticus was proportionally very high in both trap types （30.05% in lard-can traps baited with CO2, 33.58% in CDC miniature light traps）. The findings prove that a trap＇s design itself significantly affects not only the overall capture of mosquitoes but also the proportional representation of individual species.展开更多
Based on archival materials discovered in America,Britain,and China,this paper carefully examines the history of the short-lived and completely overlooked Department of Physics in the Premedical School of Peking Union...Based on archival materials discovered in America,Britain,and China,this paper carefully examines the history of the short-lived and completely overlooked Department of Physics in the Premedical School of Peking Union Medical College(PUMC),and uncovers this department’s extensive interconnections with its counterpart at Yenching University.This project also looks into the Master of Science(MS)program in the Department of Physics at Yenching University,which,founded in 1927,was the first graduate program in physics in China.It was this MS program that transformed the Yenching Department from a largely premedical training center serving the PUMC into a prominent cradle of Chinese physics research.This Yenching Department,despite its small faculty and brief existence,nurtured many eminent Chinese physicists,such as Chao-Ying Meng孟昭英,Wen-Yu Chang张文裕,Ming-Chen Wang王明贞,Chia-Liu Luke Yuan袁家骝,Cheng-Shu Wang(Chang)王承书,Ho-Fu Lu卢鹤绂,Ting-Sui Ke葛庭燧,Kun Huang黄昆,and Chia-Lin Hsieh谢家麟.It would have been impossible to achieve these outstanding accomplishments without the effective leadership and pioneering work of C.H.Corbett,P.A.Anderson,Y.M.Hsieh,and W.Band—the first four Department Chairs—in combination with relatively abundant private funding,mostly from America,and extensive educational connections with Western institutions.This paper not only introduces in detail these four department heads and their contributions,but also discloses the unique role played by the Rockefeller Foundation via the China Medical Board and the PUMC in the development of the Yenching Department of Physics.展开更多
基金Supported by National Natural Science Foundation of China(30870199)Shandong Natural Science Foundation(Y2007D34+1 种基金ZR2011CM006)Key Projects of Shandong Natural Science Foundation(2010GNC10937)~~
文摘[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were designed based on EST sequences at 5’ region of Syntaxin, and cDNA obtained through reverse reaction was taken as the template. Sequences of Syntaxin gene at 3’ region were obtained through two rounds of PCR amplifications. [Result] DNA fragments （1 096 bp） were obtained. For LsSyntaxin, open reading frame （ORF） was 816 bp and the encoded amino acids were 271. The relative molecular weight of Syntaxin was 30 254.3 Da and isoelectric point in theory was 5.55. [Conclusion] Syntaxin genes from Limonium sinense Kuntze were cloned. The research laid foundation for the study on Syntaxin gene function in Limonium sinense Kuntze and salt-secreted process.
基金Supported by National R&D Special Fund for Public Welfare Industry of China(201003070)National Natural Science Foundation of China (NSFC J0730641)~~
文摘[Objective] This study aimed to observe and explore the behavior of Procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experiment in a relatively small polyculture system. The agonistic behavior of P. clarkii and E. sinensis in the first 30 min of the initial encounter and the fighting results were observed and recorded; the approach-avoidance behavior of P. clarkii to bigger E. sinensis in a relatively large space （the ＂Y＂ maze） was observed and recorded. [Result] The re- sults showed that the times of first aggressions and approaches and the total times of active aggressions and approaches of P. clarkii in the initial period of polyculture experiment were all significantly more than E. sinensis （/：＇〈0.05）; after 30 d of poly- culture, P. clarkfi died in 30% of the groups while E. sinensis died in only 10% of the groups, and they coexisted in 60% of the groups. In the experiment of ap- proach-avoidance behavior of P. clarkii to bigger E. sinensis, with the increase of experiment times, the tendency of approach and avoidance of P. clarkii to the con- trol and E. sinensis became control-prefer （P〈0.05） from insignificantly different （P〉 0.05）, which indicated that the recognition ability of P. clarkii for E. sinensis was sig- nificantly improved, and the selection behavior of E. sinensis was obviously inhibited. [Conclusion] Presumably, polyculture of crayfishes and crabs can achieve better effi- ciency when under appropriate habitat conditions.
文摘During 2009 and 2010, 23 night-time mosquito captures were made at Kanci obora in south-eastern Moravia, Czech Republic. It was used in the Centers for Disease Control and Prevention （CDCP） miniature light traps with CO2 （dry ice） and baited lard-can traps in which sentinel animals were replaced with a container filled with CO2 （dry ice）. In the observed period, a total of 31,882 female mosquitoes were captured by CDC miniature light traps with CO2. Lard-can traps baited with CO2 captured 995 females under the same conditions, which is just 3.12% of the quantity from the CDC traps. At the same time, there were significant differences in the proportional captures of various species. Compared to CDC miniature light traps, baited lard-can traps much more often captured Aedes cinereus （16.58% of total versus 1.93% in CDC traps）, Culex modestus （15.48% versus 4.62%）, and Ae. rossicus （6.13% versus 2.67%）. On the other hand, capture of female Ae. vexans was proportionally much lower （15.38% versus 36.41%）. Capture of Cx. pipiens was more or less the same 14.77% （miniature light traps） and 15.76% （baited lard-can traps）. The occurrence of the calamity species Ae. sticticus was proportionally very high in both trap types （30.05% in lard-can traps baited with CO2, 33.58% in CDC miniature light traps）. The findings prove that a trap＇s design itself significantly affects not only the overall capture of mosquitoes but also the proportional representation of individual species.
文摘Based on archival materials discovered in America,Britain,and China,this paper carefully examines the history of the short-lived and completely overlooked Department of Physics in the Premedical School of Peking Union Medical College(PUMC),and uncovers this department’s extensive interconnections with its counterpart at Yenching University.This project also looks into the Master of Science(MS)program in the Department of Physics at Yenching University,which,founded in 1927,was the first graduate program in physics in China.It was this MS program that transformed the Yenching Department from a largely premedical training center serving the PUMC into a prominent cradle of Chinese physics research.This Yenching Department,despite its small faculty and brief existence,nurtured many eminent Chinese physicists,such as Chao-Ying Meng孟昭英,Wen-Yu Chang张文裕,Ming-Chen Wang王明贞,Chia-Liu Luke Yuan袁家骝,Cheng-Shu Wang(Chang)王承书,Ho-Fu Lu卢鹤绂,Ting-Sui Ke葛庭燧,Kun Huang黄昆,and Chia-Lin Hsieh谢家麟.It would have been impossible to achieve these outstanding accomplishments without the effective leadership and pioneering work of C.H.Corbett,P.A.Anderson,Y.M.Hsieh,and W.Band—the first four Department Chairs—in combination with relatively abundant private funding,mostly from America,and extensive educational connections with Western institutions.This paper not only introduces in detail these four department heads and their contributions,but also discloses the unique role played by the Rockefeller Foundation via the China Medical Board and the PUMC in the development of the Yenching Department of Physics.
