The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a ca...The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a carnivore.In order to reveal the relationship between digestive enzyme activities and diets in the two species,the activities of protease(P),non-specific bile salt-activated lipase(BAL) and α-amylase(A) were determined in the stomach and intestine of adult mudskipper B.pectinirostris and Chinese black sleeper B.sinensis.The results showed that the activities of protease,BAL and α-amylase in the intestine of B.pectinirostris were significantly(P<0.05) higher than those in the stomach.In B.sinensis,gastric protease activity was not different from the intestinal protease(P>0.05),while BAL and α-amylase activities of the intestine were significantly(P<0.05) higher than those of the stomach.The activity of gastric protease in B.sinensis was significantly(P<0.05) higher than that in B.pectinirostris,while the activities of intestinal protease were not different between the two fish species(P>0.05).BAL activities of the stomach and intestine in B.sinensis were significantly(P<0.05) higher than those in B.pectinirostris,while α-amylase activities of the stomach and intestine in B.pectinirostris were significantly(P<0.05) higher than those in B.sinensis.The ratios of P/BAL,A/P and A/BAL of the digestive tract in B.pectinirostris were 1.5,107.3 and 158.6,respectively;and those in B.sinensis were 0.2,1.6 and 0.2,respectively.It can be concluded that food digestion in the adult B.pectinirostris is mainly carried out in the intestine,whereas in the adult B.sinensis it is initiated in the stomach and finishes in the intestine.The activities of BAL and α-amylase in B.pectinirostris and B.sinensis are well correlated with their diets.However,a clear-cut correlation between protease activity and diets is not found in these two species.展开更多
A total of 15 pools were selected from a greenhouse, and they were randomly and evenly divided into three groups.In each group, one stocking density of Eriocheir sinensis was arranged. The results showed that after 45...A total of 15 pools were selected from a greenhouse, and they were randomly and evenly divided into three groups.In each group, one stocking density of Eriocheir sinensis was arranged. The results showed that after 45-d culture, the crab number per kilogram reached about 300 with survival rate of about 20%; the initial stocking density had significantly effect on the body size of E. sinensis on sell, instead on the survival rate of E. sinensis larvae. Therefore, it is feasible to conduct the culture of E. sinensis larvae in a pool in greenhouse.展开更多
基金Supported by Natural Science Foundation of China(No.40476056)the Key Projects of Science and Technology of Fujian Province(2003,No.262004SZ01-02)
文摘The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone.However,both species have their own unique diet.The former is an herbivore and the latter is a carnivore.In order to reveal the relationship between digestive enzyme activities and diets in the two species,the activities of protease(P),non-specific bile salt-activated lipase(BAL) and α-amylase(A) were determined in the stomach and intestine of adult mudskipper B.pectinirostris and Chinese black sleeper B.sinensis.The results showed that the activities of protease,BAL and α-amylase in the intestine of B.pectinirostris were significantly(P<0.05) higher than those in the stomach.In B.sinensis,gastric protease activity was not different from the intestinal protease(P>0.05),while BAL and α-amylase activities of the intestine were significantly(P<0.05) higher than those of the stomach.The activity of gastric protease in B.sinensis was significantly(P<0.05) higher than that in B.pectinirostris,while the activities of intestinal protease were not different between the two fish species(P>0.05).BAL activities of the stomach and intestine in B.sinensis were significantly(P<0.05) higher than those in B.pectinirostris,while α-amylase activities of the stomach and intestine in B.pectinirostris were significantly(P<0.05) higher than those in B.sinensis.The ratios of P/BAL,A/P and A/BAL of the digestive tract in B.pectinirostris were 1.5,107.3 and 158.6,respectively;and those in B.sinensis were 0.2,1.6 and 0.2,respectively.It can be concluded that food digestion in the adult B.pectinirostris is mainly carried out in the intestine,whereas in the adult B.sinensis it is initiated in the stomach and finishes in the intestine.The activities of BAL and α-amylase in B.pectinirostris and B.sinensis are well correlated with their diets.However,a clear-cut correlation between protease activity and diets is not found in these two species.
基金Supported by Fund for Hubei Modern Agricultural Industry Technology System
文摘A total of 15 pools were selected from a greenhouse, and they were randomly and evenly divided into three groups.In each group, one stocking density of Eriocheir sinensis was arranged. The results showed that after 45-d culture, the crab number per kilogram reached about 300 with survival rate of about 20%; the initial stocking density had significantly effect on the body size of E. sinensis on sell, instead on the survival rate of E. sinensis larvae. Therefore, it is feasible to conduct the culture of E. sinensis larvae in a pool in greenhouse.
