AIM: To examine the growth inhibitory effects of Phyllanthus emblica (P. emblica) and Terminalia bellerica (T. bellerica) extracts on human hepatocellular carcinoma (HepG2), and lung carcinoma (A549) cells and their s...AIM: To examine the growth inhibitory effects of Phyllanthus emblica (P. emblica) and Terminalia bellerica (T. bellerica) extracts on human hepatocellular carcinoma (HepG2), and lung carcinoma (A549) cells and their synergistic effect with doxorubicin or cisplatin. METHODS: HepG2 and A549 cells were treated with P. emblica and T. bellerica extracts either alone or in combination with doxorubicin or cisplatin and effects on cell growth were determined using the sulforhodamine B (SRB) assay. The isobologram and combination index (CI) method of Chou-Talalay were used to evaluate interactions between plant extracts and drugs. RESULTS: P. emblica and T. bellerica extracts demonstrated growth inhibitory activity, with a certain degree of selectivity against the two cancer cell lines tested. Synergistic effects (CI < 1) for P. emblica /doxorubicin or cisplatin at different dose levels were demonstrated in A549 and HepG2 cells. The T. bellerica/ cisplatin or doxorubicin also showed synergistic effects in A549 and HepG2 cells. In some instances, the combinations resulted in antagonistic effects. The dose reduction level was different and specific to each combination and cell line. CONCLUSION: The growth inhibitory activity of doxorubicin or cisplatin, as a single agent, may be modified by combinations of P. emblica or T. bellerica extracts and be synergistically enhanced in some cases. Depending on the combination ratio, the doses for each drug for a given degree of effect in the combination may be reduced. The mechanisms involved in this interaction between chemotherapeutic drugs and plant extracts remain unclear and should be further evaluated.展开更多
Objective To explore the inhibitory effect of recombinant mutant human tumor necrosis factor-α(rmhTNF-α) in combination with cisplatin on human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma c...Objective To explore the inhibitory effect of recombinant mutant human tumor necrosis factor-α(rmhTNF-α) in combination with cisplatin on human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma cell line A549 was treated with varying concentrations of rmhTNF-α(0.38, 0.75, 1.50, 6.00 and 12.00 IU/ml) or cisplatin(3.91, 7.81, 15.63, 31.25 and 62.50 μg/ml) for 24 hours. Viable cell number was analyzed by using crystal violet staining. The inhibitory rates of A549 cells growth by the two drugs were calculated. For analyzing whether there was a synergistic effect of rmhTNF-α with cisplatin, A549 cells were treated with 0.75 IU/ml rmhTNF-α and increased concentrations of cisplatin. Results rmhTNF-α or cisplatin inhibited the growth of A549 cell lines in a dose-dependent manner. The inhibitory effect of rmhTNF-α combined with cisplatin was significantly greater than cisplatin alone at the same concentration(all P<0.01). Conclusion rmhTNF-α combined with cisplatin might have synergistic inhibitory effect on human lung adenocarcinoma cell line A549.展开更多
The effects of salinity （50 mmol/L NaCI） and Cd （1 μmol/L CdCl2） as sole and combined on growth and photosynthetic parameters were studied using two soybean genotypes, Huachun 18 and NGB. The concentrations of C...The effects of salinity （50 mmol/L NaCI） and Cd （1 μmol/L CdCl2） as sole and combined on growth and photosynthetic parameters were studied using two soybean genotypes, Huachun 18 and NGB. The concentrations of Cd^2＋, Zn^2＋, Ca^2＋, Mg^2＋, K^＋ and Na^＋were also determined in seeds and pods. Huachun 18 suffered a more serious decrease than NGB in net photosynthetic rate （P,） in the treatments of salinity stress alone and combined stress （NaCl＋Cd）, showing that it is relatively sensitive to salinity. The decrease in P, caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency （the ratio of variable fluorescence to maximal fluorescence, Fv/Fm）, whereas the decease in NGB was mainly related to reduced stomatal conductance （Gs）, The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress alone. Greater change in the pod concentrations of Zn^2＋, Ca^2＋, Mg^2＋, K^＋ and Na^＋was detected under salt stress for Huachun 18 than for NGB. The results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes.展开更多
The cooperation effects of GA3, IAA and uniconazole-P were studied on the gravitropism and wood formation in Fraxinus mandshurica Rupr. var.japonica Maxim. Seedlings using traditional paraffin section technology. Our ...The cooperation effects of GA3, IAA and uniconazole-P were studied on the gravitropism and wood formation in Fraxinus mandshurica Rupr. var.japonica Maxim. Seedlings using traditional paraffin section technology. Our results are： （1） Gravitropism of stems was strongly inhibited only in B, whereas promoted significantly in D, E, F, H and I treatments; （2） Xylem formation was increased on both sides in H, I and J treatments and on the lower side in E and F which also show the synergistic effect; （3） On the radial direction, cell wall thickness was enhanced on the upper side, whereas decreased on the lower side in C, and was also significantly promoted on the upper in E, G, H, I and J treatments. On the tangential direction, cell wall thickness was increased on the lower side in E, H, I and J treatments respectively; （4） Gelatinous layer of wood fibers was observed on the upper side in all treatments. These results suggest that both GA3 and IAA affected negative gravitropism and wood formation significantly. However, appliedor unapplited-uniconazole-P does not affect G-layer formation, indicating GA does not play the key role on G-layer formation, and ratio of GA3/IAA or IAA may be more important in regulating G-layer formation.展开更多
基金research grants from Thammasat University, Thailand
文摘AIM: To examine the growth inhibitory effects of Phyllanthus emblica (P. emblica) and Terminalia bellerica (T. bellerica) extracts on human hepatocellular carcinoma (HepG2), and lung carcinoma (A549) cells and their synergistic effect with doxorubicin or cisplatin. METHODS: HepG2 and A549 cells were treated with P. emblica and T. bellerica extracts either alone or in combination with doxorubicin or cisplatin and effects on cell growth were determined using the sulforhodamine B (SRB) assay. The isobologram and combination index (CI) method of Chou-Talalay were used to evaluate interactions between plant extracts and drugs. RESULTS: P. emblica and T. bellerica extracts demonstrated growth inhibitory activity, with a certain degree of selectivity against the two cancer cell lines tested. Synergistic effects (CI < 1) for P. emblica /doxorubicin or cisplatin at different dose levels were demonstrated in A549 and HepG2 cells. The T. bellerica/ cisplatin or doxorubicin also showed synergistic effects in A549 and HepG2 cells. In some instances, the combinations resulted in antagonistic effects. The dose reduction level was different and specific to each combination and cell line. CONCLUSION: The growth inhibitory activity of doxorubicin or cisplatin, as a single agent, may be modified by combinations of P. emblica or T. bellerica extracts and be synergistically enhanced in some cases. Depending on the combination ratio, the doses for each drug for a given degree of effect in the combination may be reduced. The mechanisms involved in this interaction between chemotherapeutic drugs and plant extracts remain unclear and should be further evaluated.
文摘Objective To explore the inhibitory effect of recombinant mutant human tumor necrosis factor-α(rmhTNF-α) in combination with cisplatin on human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma cell line A549 was treated with varying concentrations of rmhTNF-α(0.38, 0.75, 1.50, 6.00 and 12.00 IU/ml) or cisplatin(3.91, 7.81, 15.63, 31.25 and 62.50 μg/ml) for 24 hours. Viable cell number was analyzed by using crystal violet staining. The inhibitory rates of A549 cells growth by the two drugs were calculated. For analyzing whether there was a synergistic effect of rmhTNF-α with cisplatin, A549 cells were treated with 0.75 IU/ml rmhTNF-α and increased concentrations of cisplatin. Results rmhTNF-α or cisplatin inhibited the growth of A549 cell lines in a dose-dependent manner. The inhibitory effect of rmhTNF-α combined with cisplatin was significantly greater than cisplatin alone at the same concentration(all P<0.01). Conclusion rmhTNF-α combined with cisplatin might have synergistic inhibitory effect on human lung adenocarcinoma cell line A549.
基金Project (No. Z304104) supported by Natural Science Foundation of Zhejiang Province, China
文摘The effects of salinity （50 mmol/L NaCI） and Cd （1 μmol/L CdCl2） as sole and combined on growth and photosynthetic parameters were studied using two soybean genotypes, Huachun 18 and NGB. The concentrations of Cd^2＋, Zn^2＋, Ca^2＋, Mg^2＋, K^＋ and Na^＋were also determined in seeds and pods. Huachun 18 suffered a more serious decrease than NGB in net photosynthetic rate （P,） in the treatments of salinity stress alone and combined stress （NaCl＋Cd）, showing that it is relatively sensitive to salinity. The decrease in P, caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency （the ratio of variable fluorescence to maximal fluorescence, Fv/Fm）, whereas the decease in NGB was mainly related to reduced stomatal conductance （Gs）, The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress alone. Greater change in the pod concentrations of Zn^2＋, Ca^2＋, Mg^2＋, K^＋ and Na^＋was detected under salt stress for Huachun 18 than for NGB. The results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes.
基金This research was supported by the scholarship from the Japanese Ministry of Education （No. 07456073）, Scientific Research Foundation for the Returned Oversea Chinese Scholars, State Education Ministry of China, Natural Science Foundation of Tianjin, China （No. 07JCYBJCI2400 and No. 07JCYBJCI2500） and National Key Basic Research Plan Proiect （No. 2007CB 106802）.
文摘The cooperation effects of GA3, IAA and uniconazole-P were studied on the gravitropism and wood formation in Fraxinus mandshurica Rupr. var.japonica Maxim. Seedlings using traditional paraffin section technology. Our results are： （1） Gravitropism of stems was strongly inhibited only in B, whereas promoted significantly in D, E, F, H and I treatments; （2） Xylem formation was increased on both sides in H, I and J treatments and on the lower side in E and F which also show the synergistic effect; （3） On the radial direction, cell wall thickness was enhanced on the upper side, whereas decreased on the lower side in C, and was also significantly promoted on the upper in E, G, H, I and J treatments. On the tangential direction, cell wall thickness was increased on the lower side in E, H, I and J treatments respectively; （4） Gelatinous layer of wood fibers was observed on the upper side in all treatments. These results suggest that both GA3 and IAA affected negative gravitropism and wood formation significantly. However, appliedor unapplited-uniconazole-P does not affect G-layer formation, indicating GA does not play the key role on G-layer formation, and ratio of GA3/IAA or IAA may be more important in regulating G-layer formation.
