Compared to other plants, the traditional protocols for RNA isolation from sugarcane （Saccharum） are usually cJeficient and result in poor yields, or low-quality. Here we describe a modified method for isolating RNA...Compared to other plants, the traditional protocols for RNA isolation from sugarcane （Saccharum） are usually cJeficient and result in poor yields, or low-quality. Here we describe a modified method for isolating RNA from various fissoes of S. officinarum, in which only Trizol and some routine reagents were needed. The absorbance at 260/280 nm ranges from 1.70 to 1.80, indicating that the quality of RNA is high, as confirmed by double-stranded cDNA synthesis and reverse ~ranscription-polymerase chain （RT-PCR）. This protocol can also be used effectively in RNA isolation from Erianthus arundinaceus, rice （Oryza sativa Linnaeus）, Achyranthes, sporotrichum spp., Thermophil and loach （Misgurnus an g uillicaudatus ）.展开更多
The most recognized and employed model of the solvation equilibration in the ionic solutions was proposed by Eigen and Tamm, in which there are four major states for an ion pair in the solution： the completely solvat...The most recognized and employed model of the solvation equilibration in the ionic solutions was proposed by Eigen and Tamm, in which there are four major states for an ion pair in the solution： the completely solvated state, 2SIP （double solvent separate ion pair）, SIP （single solvent separate ion pair）, and CIP （contact ion pair）. Eigen and Tamm suggested that the transition from SIP to CIP is always the slowest step during the whole pairing process, due to a high free energy barrier between these two states. We carried out a series of potential of mean force calculations to study the pairing free energy profiles of two sets of model mono- atomic 1：1 ion pairs 2.0：x and x：2.0. For 2.0：x pairs the free energy barrier between the SIP and CIP states is largely reduced due to the salvation shell water structure. For these pairs the SIP to CIP transition is thus not the slowest step in the ion pair formation course. This is a deviation from the Eigen-Tamm model.展开更多
基金This research was financially supported by the National Natural Science Foundation of China （30170639） and the China High Technology （863） Project （2007AA100701）.
文摘Compared to other plants, the traditional protocols for RNA isolation from sugarcane （Saccharum） are usually cJeficient and result in poor yields, or low-quality. Here we describe a modified method for isolating RNA from various fissoes of S. officinarum, in which only Trizol and some routine reagents were needed. The absorbance at 260/280 nm ranges from 1.70 to 1.80, indicating that the quality of RNA is high, as confirmed by double-stranded cDNA synthesis and reverse ~ranscription-polymerase chain （RT-PCR）. This protocol can also be used effectively in RNA isolation from Erianthus arundinaceus, rice （Oryza sativa Linnaeus）, Achyranthes, sporotrichum spp., Thermophil and loach （Misgurnus an g uillicaudatus ）.
文摘The most recognized and employed model of the solvation equilibration in the ionic solutions was proposed by Eigen and Tamm, in which there are four major states for an ion pair in the solution： the completely solvated state, 2SIP （double solvent separate ion pair）, SIP （single solvent separate ion pair）, and CIP （contact ion pair）. Eigen and Tamm suggested that the transition from SIP to CIP is always the slowest step during the whole pairing process, due to a high free energy barrier between these two states. We carried out a series of potential of mean force calculations to study the pairing free energy profiles of two sets of model mono- atomic 1：1 ion pairs 2.0：x and x：2.0. For 2.0：x pairs the free energy barrier between the SIP and CIP states is largely reduced due to the salvation shell water structure. For these pairs the SIP to CIP transition is thus not the slowest step in the ion pair formation course. This is a deviation from the Eigen-Tamm model.
