株型是影响谷类作物产量的重要性状,株型改良对提高作物产量具有重要意义。独脚金内酯(strigolactones,SLs)作为一种最新被鉴定的植物激素,其通过抑制腋芽的伸长调控分枝/分蘖的形成。β-胡萝卜素异构酶(D27s)是SLs合成途径的关键酶,通...株型是影响谷类作物产量的重要性状,株型改良对提高作物产量具有重要意义。独脚金内酯(strigolactones,SLs)作为一种最新被鉴定的植物激素,其通过抑制腋芽的伸长调控分枝/分蘖的形成。β-胡萝卜素异构酶(D27s)是SLs合成途径的关键酶,通过对谷子(Setaria italica)β-胡萝卜素异构酶典型结构域Pfam:DUF4033进行分析,鉴定到3个谷子D27s基因家族成员(Seita.8G168400、Seita.6G088800和Seita.3G050900)。蛋白质特性分析显示,谷子D27s蛋白由271–277个氨基酸残基组成,分子量为30.1–30.4 k Da,等电点为5.85–9.31,不稳定系数介于38.48–74.47之间,且均定位于叶绿体;系统进化分析发现,谷子D27s家族成员位于3个不同进化分支;顺式作用元件预测显示,SiD27-1(Seita.8G168400)可能参与调控生物节律、生长素介导的生长发育以及干旱和低温等胁迫应答过程。基因表达分析显示,SiD27-1在谷子多分蘖材料中表达下调,在低磷胁迫处理下,D27s基因均能产生不同程度的响应,并且Si D27-1的响应较其它成员更快速。单倍型分析结果表明,SiD27-1的H001单倍型为优异单倍型,对谷子的株高、抽穗期和产量改良具有重要应用价值。综上,推测SiD27-1极可能在SLs合成中发挥关键作用并对谷子株型产生影响。研究结果为深入揭示D27s对谷子分蘖形成的调控机制奠定了基础,也为谷子株型分子设计育种提供了优异的等位变异位点。展开更多
This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit （COI） gene region （450 bp） to investigate the genetic structure of the oriental river prawn （ Macrobrachium nipponense ） ...This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit （COI） gene region （450 bp） to investigate the genetic structure of the oriental river prawn （ Macrobrachium nipponense ） among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability （h = 1.000, π = 0.028）, whereas the population from Cbongqing exhibits the lowest level of variability （h = 0.700,π = 0.008）. Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder （90.34%） to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining （N J） method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.展开更多
The C++ program: Hapseeker was developed to analyze DNA or RNA sequence, besides, Hapseeker could be used to identify haplotype, calculate frequency of each haplotype as well as find variable site quickly. Moreover, H...The C++ program: Hapseeker was developed to analyze DNA or RNA sequence, besides, Hapseeker could be used to identify haplotype, calculate frequency of each haplotype as well as find variable site quickly. Moreover, Hapseeker had many advantages such as simple operation, rapid running speed and high accuracy.展开更多
文摘株型是影响谷类作物产量的重要性状,株型改良对提高作物产量具有重要意义。独脚金内酯(strigolactones,SLs)作为一种最新被鉴定的植物激素,其通过抑制腋芽的伸长调控分枝/分蘖的形成。β-胡萝卜素异构酶(D27s)是SLs合成途径的关键酶,通过对谷子(Setaria italica)β-胡萝卜素异构酶典型结构域Pfam:DUF4033进行分析,鉴定到3个谷子D27s基因家族成员(Seita.8G168400、Seita.6G088800和Seita.3G050900)。蛋白质特性分析显示,谷子D27s蛋白由271–277个氨基酸残基组成,分子量为30.1–30.4 k Da,等电点为5.85–9.31,不稳定系数介于38.48–74.47之间,且均定位于叶绿体;系统进化分析发现,谷子D27s家族成员位于3个不同进化分支;顺式作用元件预测显示,SiD27-1(Seita.8G168400)可能参与调控生物节律、生长素介导的生长发育以及干旱和低温等胁迫应答过程。基因表达分析显示,SiD27-1在谷子多分蘖材料中表达下调,在低磷胁迫处理下,D27s基因均能产生不同程度的响应,并且Si D27-1的响应较其它成员更快速。单倍型分析结果表明,SiD27-1的H001单倍型为优异单倍型,对谷子的株高、抽穗期和产量改良具有重要应用价值。综上,推测SiD27-1极可能在SLs合成中发挥关键作用并对谷子株型产生影响。研究结果为深入揭示D27s对谷子分蘖形成的调控机制奠定了基础,也为谷子株型分子设计育种提供了优异的等位变异位点。
基金This project was supported partly by Key Research Programof Zhejiang(2005C12006-01,2006C12005)
文摘This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit （COI） gene region （450 bp） to investigate the genetic structure of the oriental river prawn （ Macrobrachium nipponense ） among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability （h = 1.000, π = 0.028）, whereas the population from Cbongqing exhibits the lowest level of variability （h = 0.700,π = 0.008）. Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder （90.34%） to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining （N J） method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.
基金Supported by the Pilot Project of the Knowledge Innovation Program(0654221211)~~
文摘The C++ program: Hapseeker was developed to analyze DNA or RNA sequence, besides, Hapseeker could be used to identify haplotype, calculate frequency of each haplotype as well as find variable site quickly. Moreover, Hapseeker had many advantages such as simple operation, rapid running speed and high accuracy.
