A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named ...A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and prelimiary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required.展开更多
The optical properties of three linear conjugated oligomers （F-P, F-P-F, and P-F-P-F-P）, where phenothiazine （P） and fluorene （F） groups arrange alternately, are investigated. With the enhancement of the π-conj...The optical properties of three linear conjugated oligomers （F-P, F-P-F, and P-F-P-F-P）, where phenothiazine （P） and fluorene （F） groups arrange alternately, are investigated. With the enhancement of the π-conjugated system, their absorption and emission bands both gradually red shift, and their two-photon properties are also improved. Meanwhile, their fluorescence dynamic traces are analyzed with continuous rate distribution model, exhibiting that their decay rates gradually accelerate and the rate distribution width become narrower. The quantum chemical calculation offers their molecular structures and transition mechanism, showing that the enhancement of π-conjugated system should be responsible for tile improvement of two-photon properties.展开更多
The possibilities of changes of ORP （oxidation-reduction water potential） with the help of chemical and physical-chemical methods were explored. Distillation and membrane technology were used as physical-chemical me...The possibilities of changes of ORP （oxidation-reduction water potential） with the help of chemical and physical-chemical methods were explored. Distillation and membrane technology were used as physical-chemical methods. In the case of application of chemical methods well-soluble substances were added into water. It was ascertained that the application of membrane technology makes it possible to obtain antioxidant water with negative ORR. Different energy change in a time unit can be applied in a whole number of technological processes and reveals new possibilities for many branches of industry.展开更多
基金Supported by the National Natural Science Foundation of China(No.295256O9 and 29736180).
文摘A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and prelimiary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required.
基金ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China. （No.21103161. No.11274142, No.11304058, No.11274034. and No.11004080） and the China Postdoctoral Science Foundation （No.2011MS00927 and No.2013T60319）.
文摘The optical properties of three linear conjugated oligomers （F-P, F-P-F, and P-F-P-F-P）, where phenothiazine （P） and fluorene （F） groups arrange alternately, are investigated. With the enhancement of the π-conjugated system, their absorption and emission bands both gradually red shift, and their two-photon properties are also improved. Meanwhile, their fluorescence dynamic traces are analyzed with continuous rate distribution model, exhibiting that their decay rates gradually accelerate and the rate distribution width become narrower. The quantum chemical calculation offers their molecular structures and transition mechanism, showing that the enhancement of π-conjugated system should be responsible for tile improvement of two-photon properties.
文摘The possibilities of changes of ORP （oxidation-reduction water potential） with the help of chemical and physical-chemical methods were explored. Distillation and membrane technology were used as physical-chemical methods. In the case of application of chemical methods well-soluble substances were added into water. It was ascertained that the application of membrane technology makes it possible to obtain antioxidant water with negative ORR. Different energy change in a time unit can be applied in a whole number of technological processes and reveals new possibilities for many branches of industry.
