The 11th Five Year Plan has divided national functional zones in the basic unit of county-level administrative region.After implementation of national Major Function-oriented Zones (MFOZ) at prefecture and county leve...The 11th Five Year Plan has divided national functional zones in the basic unit of county-level administrative region.After implementation of national Major Function-oriented Zones (MFOZ) at prefecture and county levels,the spatial development sequence must be specified,development intensity must be limited,oriented zones must be designated and "red line" areas of spatial governance must be defined.In view of the above,choosing a typical county area and exploring its functional zoning after orienting its major functions will supply reference for carrying out the national spatial planning in the 12th Five Year Period,and it will also provide experience for the study of function regionalization which is the theme of MFOZ in the present academic circles.In this paper,the authors take Shangyu County as an example to study the general principles,train of thought of county-level functional regionalization in development-optimized areas,and to explore its reasonable functional zoning plan.Consequently,the authors present five principles and suggest a function-oriented zoning plan with 2 levels and 4 types.展开更多
Xichou County of Wenshan Zhuang and Miao Autonomous Prefecture in southeast Yunnan is one of the karst mountainous areas in southwest China showing typical rock desertification. During this study, we set up three soil...Xichou County of Wenshan Zhuang and Miao Autonomous Prefecture in southeast Yunnan is one of the karst mountainous areas in southwest China showing typical rock desertification. During this study, we set up three soil erosion contrast test spots at Muzhe Village, Benggu Township, Xichou County, which was the birthplace of the Xichou rock-desertified land consolidation mode. The three spots included the terrace land spot (already consolidated land), sloping land spot (unconsolidated sloping land under rock desertification), and standard runoff spot (bare land spot). In 2007, a whole-year complete observation was conducted during the rainy season and "rainfall-erosion" data were obtained for 32 times. Our analysis showed that during the entire observation period, the number of the rainfalls that led to soil erosion accounted for 34.04% of the number of all rainfalls and the amount of the rainfalls that led to soil erosion accounted for 84.17% of the total amount of all rainfalls. The average erosive rainfall standard in the three test spots was 11.0mm, slightly higher than the lO mm standard that has been adopted all over China, but lower than the 12.7 mm standard of the US and the 13.0 mm standard of Japan. According to single-factor analysis, the soil loss in the sloping land spot (L2) and that in the bare land spot (L3) are correlated to certain extent to manyother factors, including the single precipitation (P), rainfall intensity during the maximum ten minutes (Lo), rainfall intensity during the maximum 20 minutes (I2o), rainfall intensity during the maximum 30 minutes (I30), rainfall intensity during the maximum 40 minutes (I4o), and rainfall intensity during the maximum 6o minutes (I60). Among these factors, they are of the highest relativity with I6o. According to double-factor analysis, both L2 and L3 are of good relativity with P and I60. According to multi-factor analysis, L2 and L3 are also of good relativity with seven rainfall indexes, namely, P, Ia (average rainfall intensity), L10, 120, I30, 140, and I60, with their related coefficient R reaching 0.906 and 0.914, respectively. The annual soil losses in the three test spots are widely different: 1030.70 t/km2.a in the terrace land spot, which indicates a low-level erosion; 12913.22 t/km2.a in the sloping land spot (unconsolidated spot), some 12.5 times than that in the terrace land spot, which indicates an ultra-high-level erosion; and 19511.67 t/km2-a in the bare land spot, some 18.9 times than that in terrace land spot, indicating an acute erosion. These figures fully show that the Xichou rock-desertified land consolidation mode plays a significant role in soil conservation.展开更多
Objective:The aim of this study was to identify prognostic factors and imply the appropriate management for local recurrent osteosarcoma.Methods:The clinical records of 60 patients with local recurrence osteosarcoma w...Objective:The aim of this study was to identify prognostic factors and imply the appropriate management for local recurrent osteosarcoma.Methods:The clinical records of 60 patients with local recurrence osteosarcoma were reviewed between January 2002 and December 2010.The mean followed-up time for these patients was 49.1 months(range 13 to 143 months).The factors of age,gender,tumor site,tumor size,surgical procedure,neoadjuvant chemotherapy,frequency of primary postoperative adjuvant chemotherapy,lung metastasis,metastasis of other sites(except for lung) and treatment after local recurrence were selected as the measurements for this analysis.Kaplan-Meier method was used to measure the overall survival and post-recurrence survival.The univariate analysis was used to determine the prognostic factors related with survival by Log-rank test.The COX proportional-hazard regression model was used to analyze the correlation between the prognostic factor and the survival.Results:The median post-recurrence survival and overall survival of 60 patients were 32 months(95% confidence interval:16.2-47.8) and 55 months(95% confidence interval:39.3-70.7) respectively.The 2and 3-year cumulative survival rates were 81.7% and 55.4%,respectively.The Log-rank univariate analysis showed that age,gender,tumor size,metastasis of other sites(except for lung) and treatment after local recurrence were associated with the prognosis of osteosarcoma with local recurrence(P < 0.05).The Cox regression analysis revealed that gender(P = 0.016),metastasis of other sites(except for lung,P = 0.017) and treatment after local recurrence(P = 0.028) were the independent prognostic factors of osteosarcoma with local recurrence.On the other hand,the prognosis of local recurrent osteosarcoma was not associated with tumor site,surgical procedure,frequency of primary postoperative adjuvant chemotherapy,neoadjuvant chemotherapy and lung metastasis(P > 0.05).Conclusion:The independent prognostic factors for local recurrent osteosarcoma were the metastasis of other site(except for lung) and the treatment after local recurrence.The aggressive surgical treatment for local recurrence and distant metastasis could effectively improve the survival of local recurrent osteosarcoma.展开更多
This work focuses on the multicell multi-user distributed massive MIMO(DM-MIMO)systems,of which each user is equipped with single antenna and the base stations(BSs)consists of distributed antenna units. We first inves...This work focuses on the multicell multi-user distributed massive MIMO(DM-MIMO)systems,of which each user is equipped with single antenna and the base stations(BSs)consists of distributed antenna units. We first investigate the arbitrary BS antenna topology scenario. The derivation indicates that in this case the achievable uplink rate of an arbitrary user in central cell depends on both the number of BS's antennas and the users' access distance to each distributed antenna unit(DAU). As a result,the performance analysis based on the derivations is difficult. To overcome this issue and achieve clearer insight,we then consider a circularly distributed BS antenna array and obtain the asymptotic uplink rate of an arbitrary user by considering the asymptotic case where the number of antennas of BSs tends to infinity. It is achieved that the asymptotic uplink rate only depends on the distance from users' position to the center of reference cell. The presented numerical results show clearly that the distributed massive MIMO systems outperform the centralized ones. Moreover,it is also achieved that the interference from the adjacent cells imposes great impact on system performance. Besides this,in numerical analysis the averageasymptotic uplink rate of a user is presented,which is free of the users' position and only depends on the radius of circular antenna arrays. It is achieved the maximum average uplink rate would be achieved when the radius of circularly distributed antenna array goes to its optimization location.展开更多
When comparing more than two groups means, the analysis of means (ANOM) can be used as an alternative to the one-way analysis of variance (ANOVA) F test. The ANOM provides a "confidence interval type of approach...When comparing more than two groups means, the analysis of means (ANOM) can be used as an alternative to the one-way analysis of variance (ANOVA) F test. The ANOM provides a "confidence interval type of approach" that allows it to determine which, if any, of the x groups has a significantly different mean from the overall average of all the group means combined. The aim of this study was to show the application of a statistical technique (ANOM), mostly used by agriculture, to determine differences between red meat production by period in Turkey. The production quantities of goat meat, mutton, buffalo meat and beef between the years 2010 and 2016 according to the period were taken from Turkish Statistical Institute (TSI). As a result, the difference between meat productions was found to be significant.展开更多
SPSS13.0 was used to process the data of the 1-6 batch of key cultural relic conservation units promulgated by the State Administration of Cultural Heritage of China;and multi-element evaluation method was used to cal...SPSS13.0 was used to process the data of the 1-6 batch of key cultural relic conservation units promulgated by the State Administration of Cultural Heritage of China;and multi-element evaluation method was used to calculate the composite distribution index.The spatial distribution of cultural relics of provinces in China can be divided into four grades,namely:very rich areas,rich areas,poor areas and very poor areas;the article analyzes the characteristics of cultural resources in spatial and temporal distribution.The paper holds that(1)the distribution of China's cultural relic tourism resources is relatively concentrated,different sharply from region to region,with more Han nation's heritage,less relics of the ethnic minorities;(2) Henan,Hebei,Shaanxi,Shanxi Province are the main regions with heavy distribution of cultural resources;(3) relics appeared in specific time periods in China's history.Ming and Qing Dynasties,Sui,Tang and Five Dynasties,Song,Liao and Jin,the Western Han,Shang and Zhou dynasties,the New Stone Age are the major periods producing more cultural relics.This paper also analyzes relationships of the emergence of cultural relics with production technology,specific historical process,specific geographical location,political system,religion,national culture,customs,architectural styles and techniques of the traditional cultural educational system.Finally,the paper discusses the development and protection of heritage.展开更多
Part-time farming has been increasing steadily in China. It is currently the largest segment among all the farm sectors in the country. Based on rural household survey data in Taipusi County as a case site ofecologica...Part-time farming has been increasing steadily in China. It is currently the largest segment among all the farm sectors in the country. Based on rural household survey data in Taipusi County as a case site ofecologically-vulnerable areas in North China, we firstly classify farm households into four types according to the proportion of non-farm income in total income, and then compare their agricultural land use patterns to empirically examine the impacts of part-time farming on agricultural land use in this area. The results suggest that non-farming households rent out all their land and give up farming, and this satisfies the expectation of other households to expand land area. The crop planting structure was not significantly different among the households, which reflected the farmers' will to pursuit labor productivity. Part-time farming households invest more capital and materials than full-time faming households because the income derived from non-farm employment relaxes the financial constraint of households. However, the amount of labor input of part-time farming households tends to be less, and farming practices are dominated by the elderly, female and laborers withrelative low educations.Yields of crops and the benefit of agricultural land use incline to reduce, which suggest that the potential of land use productivity is more elastic to labor inputs than capital inputs in the study area.展开更多
In fracture simulation,how to model the pre-existing cracks and simulate their propagation without remeshing is an important topic.The newly developed triangular element partition method(TEPM)provides an efficient app...In fracture simulation,how to model the pre-existing cracks and simulate their propagation without remeshing is an important topic.The newly developed triangular element partition method(TEPM)provides an efficient approach to this problem.It firstly meshes the cracked body regardless of the geometry integrity of the interesting object with triangular elements.After the meshing procedure is completed,some elements are intersected by cracks.For the element intersected by a crack,the TEPM takes the element partition technique to incorporate the discontinuity into the numerical model without any interpolation enrichment.By this approach,the TEPM can simulate fracture without mesh modification.In the TEPM,all the cracked elements are treated as the usual partitioned elements in which the crack runs through.The virtual node pairs(the intersection points of crack faces and elements)at the opposite faces of the crack move independently.Their displacements are respectively determined by their neighbor real nodes(nodes formatted in the original mesh scheme)at the same side of the crack.However,among these cracked elements,the element containing a crack tip,referred to as the crack tip element thereafter,behaves differently from those cut through by the crack.Its influence on the singular field at the vicinity of the fracture tip becomes increasingly significant with the element size increasing.In the crack tip element,the virtual node pair at the crack tip move consistently before fracture occurs while the virtual node pair separate and each virtual node moves independently after the fracture propagates.Accordingly,the crack tip element is automatically transformed into the usual partitioned element.In the present paper,the crack tip element is introduced into the TEPM to account for the effect of the crack tip.Validation examples indicate that the present method is almost free from the element size effect.It can reach the same precision as the conventional finite element method under the same meshing scheme.But the TEPM is much more efficient and convenient than the conventional finite element method because the TEPM avoids the troubles that the conventional finite element method suffers,e.g.,the meshing problem of cracked body,modification of mesh scheme,etc.Though the extended finite element method can also avoid these troubles,it introduces extra degrees of freedom due to node interpolation enrichment.Due to the simplicity of the present TEPM,it is believed that its perspective should be highly inspiring.展开更多
Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a s...Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic ca- pacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some sin- gle-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a con- structed mouse naive antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from vi- rus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.展开更多
基金supported jointly by the National Basic Research Program of MOST of China (Grant No.2008BAH31B06)the Key Program of NNSF of China (GrantNo. 40830741)
文摘The 11th Five Year Plan has divided national functional zones in the basic unit of county-level administrative region.After implementation of national Major Function-oriented Zones (MFOZ) at prefecture and county levels,the spatial development sequence must be specified,development intensity must be limited,oriented zones must be designated and "red line" areas of spatial governance must be defined.In view of the above,choosing a typical county area and exploring its functional zoning after orienting its major functions will supply reference for carrying out the national spatial planning in the 12th Five Year Period,and it will also provide experience for the study of function regionalization which is the theme of MFOZ in the present academic circles.In this paper,the authors take Shangyu County as an example to study the general principles,train of thought of county-level functional regionalization in development-optimized areas,and to explore its reasonable functional zoning plan.Consequently,the authors present five principles and suggest a function-oriented zoning plan with 2 levels and 4 types.
基金funded by the National Natural Science Foundation of China (No. 40661010)
文摘Xichou County of Wenshan Zhuang and Miao Autonomous Prefecture in southeast Yunnan is one of the karst mountainous areas in southwest China showing typical rock desertification. During this study, we set up three soil erosion contrast test spots at Muzhe Village, Benggu Township, Xichou County, which was the birthplace of the Xichou rock-desertified land consolidation mode. The three spots included the terrace land spot (already consolidated land), sloping land spot (unconsolidated sloping land under rock desertification), and standard runoff spot (bare land spot). In 2007, a whole-year complete observation was conducted during the rainy season and "rainfall-erosion" data were obtained for 32 times. Our analysis showed that during the entire observation period, the number of the rainfalls that led to soil erosion accounted for 34.04% of the number of all rainfalls and the amount of the rainfalls that led to soil erosion accounted for 84.17% of the total amount of all rainfalls. The average erosive rainfall standard in the three test spots was 11.0mm, slightly higher than the lO mm standard that has been adopted all over China, but lower than the 12.7 mm standard of the US and the 13.0 mm standard of Japan. According to single-factor analysis, the soil loss in the sloping land spot (L2) and that in the bare land spot (L3) are correlated to certain extent to manyother factors, including the single precipitation (P), rainfall intensity during the maximum ten minutes (Lo), rainfall intensity during the maximum 20 minutes (I2o), rainfall intensity during the maximum 30 minutes (I30), rainfall intensity during the maximum 40 minutes (I4o), and rainfall intensity during the maximum 6o minutes (I60). Among these factors, they are of the highest relativity with I6o. According to double-factor analysis, both L2 and L3 are of good relativity with P and I60. According to multi-factor analysis, L2 and L3 are also of good relativity with seven rainfall indexes, namely, P, Ia (average rainfall intensity), L10, 120, I30, 140, and I60, with their related coefficient R reaching 0.906 and 0.914, respectively. The annual soil losses in the three test spots are widely different: 1030.70 t/km2.a in the terrace land spot, which indicates a low-level erosion; 12913.22 t/km2.a in the sloping land spot (unconsolidated spot), some 12.5 times than that in the terrace land spot, which indicates an ultra-high-level erosion; and 19511.67 t/km2-a in the bare land spot, some 18.9 times than that in terrace land spot, indicating an acute erosion. These figures fully show that the Xichou rock-desertified land consolidation mode plays a significant role in soil conservation.
文摘Objective:The aim of this study was to identify prognostic factors and imply the appropriate management for local recurrent osteosarcoma.Methods:The clinical records of 60 patients with local recurrence osteosarcoma were reviewed between January 2002 and December 2010.The mean followed-up time for these patients was 49.1 months(range 13 to 143 months).The factors of age,gender,tumor site,tumor size,surgical procedure,neoadjuvant chemotherapy,frequency of primary postoperative adjuvant chemotherapy,lung metastasis,metastasis of other sites(except for lung) and treatment after local recurrence were selected as the measurements for this analysis.Kaplan-Meier method was used to measure the overall survival and post-recurrence survival.The univariate analysis was used to determine the prognostic factors related with survival by Log-rank test.The COX proportional-hazard regression model was used to analyze the correlation between the prognostic factor and the survival.Results:The median post-recurrence survival and overall survival of 60 patients were 32 months(95% confidence interval:16.2-47.8) and 55 months(95% confidence interval:39.3-70.7) respectively.The 2and 3-year cumulative survival rates were 81.7% and 55.4%,respectively.The Log-rank univariate analysis showed that age,gender,tumor size,metastasis of other sites(except for lung) and treatment after local recurrence were associated with the prognosis of osteosarcoma with local recurrence(P < 0.05).The Cox regression analysis revealed that gender(P = 0.016),metastasis of other sites(except for lung,P = 0.017) and treatment after local recurrence(P = 0.028) were the independent prognostic factors of osteosarcoma with local recurrence.On the other hand,the prognosis of local recurrent osteosarcoma was not associated with tumor site,surgical procedure,frequency of primary postoperative adjuvant chemotherapy,neoadjuvant chemotherapy and lung metastasis(P > 0.05).Conclusion:The independent prognostic factors for local recurrent osteosarcoma were the metastasis of other site(except for lung) and the treatment after local recurrence.The aggressive surgical treatment for local recurrence and distant metastasis could effectively improve the survival of local recurrent osteosarcoma.
基金supported by the Natural Science Foundation of China under Grant 61261015 and 61561043the 973 project 2013CB329104,the Natural Science Foundation of China under Grant 61372124,61363059,and 61302100+1 种基金the projects BK2011027,the Natural Science Foundation of Gansu Province for Distinguished Young Scholars(1308RJDA007)by the Foundation Research Funds for the University of Gansu Province:‘Massive MIMO channels modeling and estimation over millimeter wave band for 5G’
文摘This work focuses on the multicell multi-user distributed massive MIMO(DM-MIMO)systems,of which each user is equipped with single antenna and the base stations(BSs)consists of distributed antenna units. We first investigate the arbitrary BS antenna topology scenario. The derivation indicates that in this case the achievable uplink rate of an arbitrary user in central cell depends on both the number of BS's antennas and the users' access distance to each distributed antenna unit(DAU). As a result,the performance analysis based on the derivations is difficult. To overcome this issue and achieve clearer insight,we then consider a circularly distributed BS antenna array and obtain the asymptotic uplink rate of an arbitrary user by considering the asymptotic case where the number of antennas of BSs tends to infinity. It is achieved that the asymptotic uplink rate only depends on the distance from users' position to the center of reference cell. The presented numerical results show clearly that the distributed massive MIMO systems outperform the centralized ones. Moreover,it is also achieved that the interference from the adjacent cells imposes great impact on system performance. Besides this,in numerical analysis the averageasymptotic uplink rate of a user is presented,which is free of the users' position and only depends on the radius of circular antenna arrays. It is achieved the maximum average uplink rate would be achieved when the radius of circularly distributed antenna array goes to its optimization location.
文摘When comparing more than two groups means, the analysis of means (ANOM) can be used as an alternative to the one-way analysis of variance (ANOVA) F test. The ANOM provides a "confidence interval type of approach" that allows it to determine which, if any, of the x groups has a significantly different mean from the overall average of all the group means combined. The aim of this study was to show the application of a statistical technique (ANOM), mostly used by agriculture, to determine differences between red meat production by period in Turkey. The production quantities of goat meat, mutton, buffalo meat and beef between the years 2010 and 2016 according to the period were taken from Turkish Statistical Institute (TSI). As a result, the difference between meat productions was found to be significant.
基金supported by Science Research Program of Hainan Provincial Education Bureau (Grant No Hj2008-79)funded by the National Natural Science Foundation of China (Grant Nos 40963001, 40061003, 40661013)Key Course Program of Natural Geography of Hainan Normal University and First-degree Course Program of Geographical Science of Hainan Province
文摘SPSS13.0 was used to process the data of the 1-6 batch of key cultural relic conservation units promulgated by the State Administration of Cultural Heritage of China;and multi-element evaluation method was used to calculate the composite distribution index.The spatial distribution of cultural relics of provinces in China can be divided into four grades,namely:very rich areas,rich areas,poor areas and very poor areas;the article analyzes the characteristics of cultural resources in spatial and temporal distribution.The paper holds that(1)the distribution of China's cultural relic tourism resources is relatively concentrated,different sharply from region to region,with more Han nation's heritage,less relics of the ethnic minorities;(2) Henan,Hebei,Shaanxi,Shanxi Province are the main regions with heavy distribution of cultural resources;(3) relics appeared in specific time periods in China's history.Ming and Qing Dynasties,Sui,Tang and Five Dynasties,Song,Liao and Jin,the Western Han,Shang and Zhou dynasties,the New Stone Age are the major periods producing more cultural relics.This paper also analyzes relationships of the emergence of cultural relics with production technology,specific historical process,specific geographical location,political system,religion,national culture,customs,architectural styles and techniques of the traditional cultural educational system.Finally,the paper discusses the development and protection of heritage.
基金the National Natural Science Foundation of China (Grant no. 40971062)
文摘Part-time farming has been increasing steadily in China. It is currently the largest segment among all the farm sectors in the country. Based on rural household survey data in Taipusi County as a case site ofecologically-vulnerable areas in North China, we firstly classify farm households into four types according to the proportion of non-farm income in total income, and then compare their agricultural land use patterns to empirically examine the impacts of part-time farming on agricultural land use in this area. The results suggest that non-farming households rent out all their land and give up farming, and this satisfies the expectation of other households to expand land area. The crop planting structure was not significantly different among the households, which reflected the farmers' will to pursuit labor productivity. Part-time farming households invest more capital and materials than full-time faming households because the income derived from non-farm employment relaxes the financial constraint of households. However, the amount of labor input of part-time farming households tends to be less, and farming practices are dominated by the elderly, female and laborers withrelative low educations.Yields of crops and the benefit of agricultural land use incline to reduce, which suggest that the potential of land use productivity is more elastic to labor inputs than capital inputs in the study area.
基金supported by the National Natural Science Foundation of China (Grant No. 11172172)the National Basic Research Program of China ("973" Project) (Grant No. 2011CB013505)
文摘In fracture simulation,how to model the pre-existing cracks and simulate their propagation without remeshing is an important topic.The newly developed triangular element partition method(TEPM)provides an efficient approach to this problem.It firstly meshes the cracked body regardless of the geometry integrity of the interesting object with triangular elements.After the meshing procedure is completed,some elements are intersected by cracks.For the element intersected by a crack,the TEPM takes the element partition technique to incorporate the discontinuity into the numerical model without any interpolation enrichment.By this approach,the TEPM can simulate fracture without mesh modification.In the TEPM,all the cracked elements are treated as the usual partitioned elements in which the crack runs through.The virtual node pairs(the intersection points of crack faces and elements)at the opposite faces of the crack move independently.Their displacements are respectively determined by their neighbor real nodes(nodes formatted in the original mesh scheme)at the same side of the crack.However,among these cracked elements,the element containing a crack tip,referred to as the crack tip element thereafter,behaves differently from those cut through by the crack.Its influence on the singular field at the vicinity of the fracture tip becomes increasingly significant with the element size increasing.In the crack tip element,the virtual node pair at the crack tip move consistently before fracture occurs while the virtual node pair separate and each virtual node moves independently after the fracture propagates.Accordingly,the crack tip element is automatically transformed into the usual partitioned element.In the present paper,the crack tip element is introduced into the TEPM to account for the effect of the crack tip.Validation examples indicate that the present method is almost free from the element size effect.It can reach the same precision as the conventional finite element method under the same meshing scheme.But the TEPM is much more efficient and convenient than the conventional finite element method because the TEPM avoids the troubles that the conventional finite element method suffers,e.g.,the meshing problem of cracked body,modification of mesh scheme,etc.Though the extended finite element method can also avoid these troubles,it introduces extra degrees of freedom due to node interpolation enrichment.Due to the simplicity of the present TEPM,it is believed that its perspective should be highly inspiring.
基金supported by the National Basic Research Program of China (2009CB118701,2009CB118704)the National Natural Science Foundation of China (31072233,31172434)
文摘Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic ca- pacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some sin- gle-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a con- structed mouse naive antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from vi- rus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.