双启动如何变单启动

不少朋友在电脑安装了双系统,比如:Windows 9x/Me和Windows 2000,但有时想删除另外一个系统。下面为大家提供一些这方面的技巧。 1.删除Windows 9x/Me,保留Windows 2000 第一步:使用Windows 2000安装光盘启动系统,接着选择Repair(修复),按R键选择'要用紧急修复过程修复Windows 2000安装',再按M键选择'手动修复',

660MW超临界机组单汽泵启动的实践与探索

通过对660MW超临界机组单汽泵启动的实践,分析汽泵建立启动流量和机组启动初期的运行数据,找出汽动给水泵低转速下工作点偏离限制曲线的原因,并对限制曲线进行修正,引入"泵组限制曲线"这一概念,为实现超临界机组单汽泵顺利启动提供了控制思路。

锅炉单风机启动实践初探

锅炉启动初期,负荷不高,燃烧强度不大,炉膛qv、qs处于低水平阶段.从理论上讲,一套风机的风量不仅能满足燃烧初期对风量的需求,而且还能带一半的负荷,但在实际操作中,大多采用双风机中等风量点火启动的,相对而言,启动电耗成本偏高.为降低启动成本,巨化热电厂锅炉车间在单引送风机启动方面做了一些有益的尝试,现将几次单风机启动试验整理出来,供同行参考.

单作用阀门改双作用阀门的方法与应用

工业生产中常见到很多单作用阀门,有薄膜式的,也有活塞式的,往往这些阀门经过长期的使用会出现弹簧弹力下降,阀芯、阀杆、齿轮或者曲柄机构卡涩等现象,从而导致阀门动作不及时或者不到位。当出现这类情况,而我们手头又缺少新的备件时,为了避免生产中断造成重大损失,我们就可将单作用阀门通过气路改造更换为双作用阀门。另外,当日常维保中,电动单作用阀门故障时无备件也可通过电路的改造将阀门更换为电动双作用阀门。

葡萄糖调节蛋白78研究进展

葡萄糖调节蛋白78(glucose regulated protein 78kD,GRP78)又称免疫球蛋白重链结合蛋白(immunoglobulin heavy chain binding protein,Bip),是位于内质网上重要的分子伴侣,属热休克蛋白70家族的一员,GRP78分子及其DNA分子序列结构在许多生物物种中高度保守。GRP78在内质网中参与阻止内质网新生肽聚集、调节内质网钙稳态、抗内质网相关性细胞凋亡,以及启动未折叠蛋白反应等细胞生命过程。GRP78基因启动子上存在内质网应激反应元件(ERSE)和cAMP反应元件(CRE)等特殊的顺式作用元件,特异性转录因子ATF6等与GRP78启动子上顺式作用元件发生动态结合,从而调节GRP78基础性或诱导性转录表达。近年来发现,GRP78与脂肪肝、肿瘤和神经系统等疾病的发生发展密切相关,GRP78生物学功能的研究已经引起生物学家们的广泛重视。

Isolation and Characterization of CMO Gene Promoter from Halophyte Suaeda liaotungensis K.

The 5＇-flanking proximal region of stress-induced gene encoding choline monooxygenase （CMO） was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtained. The transcription start site, which is located at 128 bp upstream to the start ATG, was predicted by the TSSP-TCM program. The functional elements were analysed by PLACE program. The obtained SICMO gene promoter contains the basic elements： TATA-box, CAAT-box, and stress-induced elements, for example, salt responsive element （GAAAAA）, cold responsive elements （CANNTG）, ABA （Abscisic Acid） responsive elements （NAACAA）, water stress element （CGGTTG）, and WUN responsive elements （GTTAGGTTC）. Isolation and analysis of the promoter of the CMO gene from S. liaotungensis lays a foundation for characterising the stress-induced promoter elements, studying the relationship between the structure and function of the promoter, and investigating the molecular mechanism of CMO gene regulation.

Association of RELN promoter SNPs with schizophrenia in the Chinese population

Previous research on gene expression analysis and association tests have suggested that RELN is a risk gene for schizophrenia in world populations.Based on the reported down-regulation of RELN in schizophrenia patients compared with normal subjects,we speculated that variants in the RELN promoter region may confer risk for schizophrenia.In this study,we investigated the associations of three SNPs in the promoter region of RELN with schizophrenia in a case-control sample from southwestern China（940 cases and 13 69 controls）.The results suggested that none of the SNPs showed significant associations in our sample,indicating the risk variants for schizophrenia in RELN may not be located in the promoter region.We also performed meta-analysis by combining our data with previously reported data on the Chinese population with a total sample size of 2 843 individuals,and the result remained nonsignificant.Collectively,our results suggested variants in the RELN promoter may not harbor risk SNPs associated with schizophrenia in the Chinese population.

IL-10 and TNF-α promoter haplotypes are associated with childhood Crohn’s disease location

AIM： To determine the distribution and frequencies of the genotypes and haplotypes of the genes encoding for the glucocorticoid receptor （GR）, the tumor necrosis factor （TNF）-α and the interleukin （IL）-10 in childhood Crohn＇s disease （CD） and to assess the impact of the corresponding DNA variants on clinical and disease phenotypes.METHODS： Ten variants in GR, TNF-a and IL-10 were genotyped in 113 childhood CD cases and 95 healthy subjects, both of French-Canadian origin.RESULTS： For the GR polymorphisms （R23K and N363S） and IL-10 variants in the 5＇flanking region （-1082 G 〉 A, -819 T 〉 C and -592 A 〉 C）, no difference was observed in allele and genotype frequencies between CD patients and controls. At the haplotype level, we found three IL-10 haplotypes previously described in Caucasians （GCC, ACC and ATA） and three novel haplotypes only present in IBD patients. When we analyzed the haplotype distribution with the anatomical location of the disease, the GCC haplotype was associated with the colonic and the ACC haplotype with the terminal ileum location, respectively. The genotyping of five polymorphisms in the promoter region of the TNF-α gene （-1031 T 〉 C, -863 A 〉 C, -857 T 〉 C, -308 A 〉 G and -238 A 〉 G） revealed a significant overrepresentation of homozygous -1031 CC among CD patients （OR = 9.9） and an association with the colonic location. For TNF-α, eleven haplotypes were inferred, including two frequent ones, TCCGG and CACGG, which were significantly observed more frequently in controls and cases, respectively.CONCLUSION： This is one of the first studies investigating the association between haplotype structure and disease location in a CD pediatric cohort. Our results will help to increase our understanding of the genetic determinants of childhood CD.

Cloning and Identification of promoter of Pseudomonas Pseudoaligenes

Promoter-probe vector pSUPV4 is used to clone the promoter of Pseudomonas pseudoalcaligenes directly in E. coli, and the recombinant pPA7, which has the highest kanamycin resistance, is obtained. Sequencing the PA7 fragment discloses several motifs similar to the conservative domains of prokaryotic promoters, including -10 box, -35 box, parallel SD fragment essential to transcription initiation, and the translation initiation site ATG. Southern blotting of PA7 indicates that the PA7 fragment comes from P. pseudoalcaligenes genome and has probably one copy. The PA7 fragment is subcloned by PCR, and the result shows that the 5’-flanking fragment from 889 to 1120 bp has promoter activity, which can be enhanced by the 0.7Kb fragment at 5’ end. The fragments of pPA7 and pPA7-2 are transferred into pseudomonas pseudoaligenes by electroporation, and the significant higher kanamycin resistance of transformants than that of control indicates that the PA7 fragment has the promoter activity in P. pseudoaligene.

TLR4 -2242 T→C variant increases transcriptional activity of its promoter

Objective:To investigate the effects of -2242,-1892 and -1837 single nucleotide polymorphisms(SNPs) on toll-like receptor 4(TLR4) promoter activity.Methods:Polymerase chain reaction(PCR) and site direct mutation technology were used to construct TLR4 basic promoter and -2242C,-1892A and -1837G mutate promoter plasmids.Dual-Luciferase Reporter assay system was used to detect the activity of constructed promoter following human embryonic kidney(HEK) 293 cells were transiently cotransfected with the constructed plasmids and the control plasmid pRL-CMV.Results:In HEK293 cells,the activity of -2242C mutate promoter was higher than -2242T promoter,and there was no significant difference when both -1892A and -1837G mutate promoter compared with -1892G and -1837A promoter,respectively.Conclusion:It is implied that -2242T→C base variation can enhance the activity of TLR4 promoter,while -1892 and -1837 SNPs have no effect on TLR4 promoter activity.

Experimental Investigation on Starting Process of Supersonic Single-stage Air Ejector

This paper deals with experimental study of flow field of starting process in two-dimensional, single-stage supersonic ejector on different air total pressure. Schlieren pictures of flow field were taken, static pressure distribu-tions on side wall were measured. The obtained results show that, on critical pressure, the starting main shock waves in ejector oscillated back and forth between the second throat and the middle section of the mixing chamber, it causes the pressure in the second half of the mixing chamber acutely fluctuated .When the working pressure of the active flow is higher than the critical starting pressure, ejector starts normally and the inner flow-field of the mixing chamber keeps stable and the shock waves in the second throat have a certain degree of oscillation . After ejector starts, the operating pressure of the active flow may be lower than the starting pressure .