单导管法射频消融单形性右室流出道室性早搏、室性心动速(附80例)

目的:评价单导管法消融单形性右室流出道室早、室速的有效性、安全性和实用性。方法:对62例心脏结构正常的右室流出道单型性室早及18例右室流出道单型性室速进行了单导管射频消融。采用起搏标测法,以起搏时与室早或室速波形态完全相同点为消融靶点。结果:80例室早或室速均起源于右室流出道,呈完全性左束支阻滞形态。消融即刻成功率93.7%(75/80),未成功的5例改为在CATO三维标测系统下消融成功,动态心电图记录消融前后室早数为(14563±2453)次/24h和(586±158)次/24h,有1例患者出现心包填塞,经穿刺置管引流后消失。随访期间有5例复发,再次用单导管法射频消融成功。结论:经单导管射频消融可有效而安全地消融心脏结构正常的右室流出道单形室早、室速,可作为症状严重、药物治疗无效或不能耐受患者的治疗选择,并且手术时间短、并发症少、大量节省费用、技术易掌握。

RNA SNP Detection Method With Improved Specificity Based on Dual-competitive-padlock-probe

Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.

伊布利特转复房颤的效果及不良反应分析

目的 观察新一代Ⅲ类抗心律失常药伊布利特转复阵发性房颤的效果、不良反应。方法 选择2019年1月-2023年3月本科室治疗的68例阵发性房颤患者,10min一次性静脉注射伊布利特1mg,观察转复率、转复时间、严重不良反应。结果 伊布利特对阵发性房颤转复成功55例,成功率80.8%,平均转复时间26.7min,最快转律时间为3min,最长转律时间138min。无器质性心脏病患者,发生1例非持续性单型性室速,4h内自行消失。合并冠心病、慢性心力衰竭患者,发生持续性单型性室速、阿斯综合征1例,电复律终止;发生非持续性单型性室速1例,4h内自行终止。未发生尖端扭转性室速,未发生严重心动过缓、加重、低血压,无死亡病例。结论 无器质性心脏病的阵发性房颤患者,使用伊布利特转律快速、有效、安全;合并冠心病、慢性心力衰竭、急性心肌梗死的患者,在急救完备的情况下,可谨慎使用。

ON INFORMATION THEORY METHOD FOR RELIABILITY ASSESSMENT OF SYSTEMS CONSISTING OF DIFFERENT SUCCESS FAILURE MODEL UNITS

Using the characteristic of addition of information quantity and the principle of equivalence of information quantity, this paper derives the general conversion formulae of the formation theory method conversion (synthesis) on the systems consisting of different success failure model units. According to the fundamental method of the unit reliability assessment, the general models of system reliability approximate lower limits are given. Finally, this paper analyses the application of the assessment method by examples, the assessment results are neither conservative nor radical and very satisfactory. The assessment method can be popularized to the systems which have fixed reliability structural models.

Unisexual Pistillate Flower Regeneration in Immature Embryo Culture of Wheat

In this experiment, floral development from tissue culture of bread wheat (Triticum aestivum L.) was investigated. Immature embryos of 45 wheat cultivars were cultured, and 11.1% of the genotypes regenerated floral organs from the calli near the bases of the green buds or plantlets regenerated. The floral buds were morphologically incomplete with the appearances of unisexual pistillate flowers which were naked, clustered with normal ovaries and exuberant feather-like stigmas, but without stamens, paleas, lemmas and glumes. The histological examination showed that the pistils originated from the meristematic cells near the green buds or plantlets, and the clustered pistils were formed by secondary pistillate regeneration. The development of the feather-like structures was earlier than that of the ovules. Biovule developed from an ovary besides normal uniovule. Statistical analysis by X 2 test for independency demonstrated highly significant difference of flower regeneration among the tested genotypes. Wheat cultivar YA-1 revealed higher percentage (44.4%) than other genotypes, and the response could well be repeated in different years. It was indicated that the floral regeneration of immature embryo explants of YA-1 is relatively stable. The frequency of floral regeneration was mainly regulated by the components in the subculture media, compared with the response of the dedifferentiation media, despite the obviously different components involving basal medium type, inorganic Fe2+ concentration and plant growth regulators. The results suggested the combination of 6-benzylaminopurine, alpha-naphthalene acetic-acid and doubled inorganic Fe2+ might be more beneficial to inducing the floral development than that of 2,4-D and normal inorganic Fe2+ concentration in subculture medium. However, both immature inflorescence and mature embryo, as cultured explants of YA-1, did not regenerate any flower organs. It is believed that the immature embryo culture of YA-1 can be used to establish ideal experimental system for the study of floral developmental mechanism in wheat.

Synthesis of Li^+ adsorbent(H_2TiO_3) and its adsorption properties

H2TiO3 was obtained from the acid-modified adsorbent precursor Li2TiO3,which was synthesized by a solid-phase reaction between TiO2 and Li2CO3.The extraction ratio of Li＋ from Li2TiO3 was 98.86%,almost with no Ti4＋ extracted.The effects of lithium titanium ratio,calcining temperature and time were investigated on the synthesis of Li2TiO3.Li2TiO3,H2TiO3 and the adsorbed Li＋ adsorbent were characterized by XRD and SEM.The lithium adsorption properties were investigated by the adsorption kinetics and adsorption isotherm.The results indicate that H2TiO3 has an excellent adsorptive capacity for Li＋.Two simplified kinetic models including the pseudo-first-order and pseudo-second-order equations were selected to follow the adsorption processes.The rate constants of adsorption for these kinetic models were calculated.The results show that the adsorption process can be described by the pseudo-second-order equation,and the process is proved to be a chemical adsorption.The adsorption process that H2TiO3 adsorbs Li＋ in LiCl solution well fits the Langmuir equation with monolayer adsorption.

Polymorphism of Exon 3 of MHC Class Ⅱ B Gene in Chinese Alligator(Alligator sinensis)

The polymorphism of MHC class II B gene in 14 Chinese alligators was analyzed, which came from three different areas： a wild population from Xuancheng, Anhui, a captive population from Changxing, Zhejiang, and a captive population from Anhui Research Center for Reproduction of Chinese Alligators. The gene fragment was amplified using a pair of specific primers designed from the MHC gene sequence of the spectacled caiman. A total of 34 sequence haplotypes of exon 3 were detected in the sampled Chinese alligators. The numbers of haplotypes of the 3 Chinese alligator populations were 15, 10, and 9, respectively. The overall estimation of the MHC polymorphism in the Chinese alligator population was higher than those in mammals and in cypdnid fish, The rates of nonsynonymous substitutions （dN） occurred at a significantly lower frequency than that of synonymous substitutions （ds）, which were not consistent with the common rule. This result might suggest that the polymorphism of exon 3 seemed not to be maintained by the balancing selection. The neutrality test of Tajima excluded the null hypothesis that the polymorphism of exon 3 was generated by a random drift, and the fact that D = -0.401 indicated an excess of rare mutations in the Chinese alligator. The nucleotide diversity of the sequences and the phylogenetic relations were also analyzed, and the results suggested that there was no significant difference in genetic diversity among the 3 populations of Chinese alligator.

Adiponectin Gene Variation -4522C/T Is Associated with Type 2 Diabetic Obesity and Insulin Resistance in Chinese

The authors investigated the possible association of -4522C/T variation of adiponectin gene with coronary heart disease （CHD） and type 2 diabetes mellitus （T2DM）. Genotyping of SNP --4522C/T in 304 patients with CHD, 389 patients with T2DM, and 405 age and sex-matched healthy control subjects was carried out by means of PCR-RFLP approach. No significant difference in the genotype or allele frequencies was found, either between patients with CHD and control subjects, or between patients with T2DM and control subjects. However, in the subgroup analysis, an association of the TAr genotype and T allele with type 2 diabetes combined with obesity （BMI ≥ 25 kg/m2） was found （P = 0.014 and P = 0.034, respectively）. Also the homeostasis model assessment of insulin resistance （HOMA-IR） in T2DM patients with T/T genotype was significantly higher than that in T2DM patients carrying C allele （P = 0.0069）. The authors＇ findings for the first time demonstrated that SNP --4522 in the adiponectin gene was associated with T2DM that combined with obesity and higher insulin resistance index in patients with T2DM. This indicated that the variation might associate with an increased susceptibility to type 2 diabetic obesity and insulin resistance. But -4522C/T polymorphism did not contribute to the susceptibility of CHD.

INCLINED LAMINAL COMBINATION MODEL OF 3D BRAIDED COMPOSITES

As an advanced composite material, the 3D braided composite has received more and more attention in foreign countries. However, it has received less attention in China. The geometric unit cell which can describe the basic structure and the relationship between the braiding angle and geometric parameters of the fabric and fiber volume ratio are given in this paper based on two 3D braiding processes, namely, the four-step and the twostep ones. Several existing mechanical models to predict groperties of the 3D braided comPOsites are discussed and their shortcomings are pointed out herein. Then a new model called the inclined laminal combination model is proposed, which is based on the classical laminated plate theory and can predict the basic mechanical behavior of the two 3D braided composites with four-step or two-step braid. In the model, each yarn in the unit cell is regarded as an inclined laminate and then a 3D analysis is performed. It is found that the predicted mechanical properties of the 3D braided composites by the proposed model are compared well with the experimental data.

Distinct toll-like receptor expression in monocytes and T cells in chronic HCV infection

AIM： Hepatitis C virus often establishes chronic infections. Recent studies suggest that viral and bacterial infections are more common in HCV-infected patients compared to controls. Pathogens are recognized by Toll-like receptors （TLRs） to shape adaptive and innate immune responses. METHODS： In this study, to infected host to recognize assess the ability of HCV-infected host to recognize invading pathogens, we investigated Toll-like receptor expression in innate （monocytes） and adaptive （T cells） immune cells by realtime PCR. RESULTS： We determined that RNA levels for TLRs 2, 6. 7, 8, 9 and 10 mRNA levels were upregulated in both monocytes and T cells in HCV-infected patients compared to controls. TLR4 was only upregulated in T lymphocytes, while TLR5 was selectively increased in monocytes of HCV-infected patients. MD-2, a TLR4 coreceptor, was increased in patients＇ monocytes and T cells while CD14 and MyD88 were increased only in monocytes. CONCLUSION： Our data reveal novel details on TLR expression that likely relates to innate recognition of pathogens and immune defense in HCV-infected individuals.

Polymorphisms of micro RNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer

AIM To evaluate associations between mi RNA target genes IL12B,INSR,CCND1 and IL10 polymorphisms and gastric cancer(GC)in European population.METHODS Gene polymorphisms were analyzed in 508 controls and474 GC patients from 3 tertiary centers in Germany,Lithuania and Latvia.Controls were patients from the out-patient departments,who were referred for upper endoscopy because of dyspeptic symptoms and had no history of previous malignancy.Gastric cancer(GC)patients had histopathological verification of gastric adenocarcinoma.Genomic DNA was extracted using salting out method from peripheral blood mononuclear cells.IL12B T>G(rs1368439),INSR T>C(rs1051690),CCND1 A>C(rs7177)and IL10 T>C(rs3024498)SNPs were genotyped by the real-time polymerase chain reaction.Associations between gene polymorphism and GC were evaluated using multiple logistic regression analysis with adjustment for sex,age and country of birth.RESULTS We observed similar distribution of genotypes and allelic frequencies of all polymorphisms between GC patients and controls except of INSR rs1051690.The frequency of the T allele of INSR gene was significantly higher in GC patients than in controls(23.26%and 19.19%respectively,P=0.028).CT genotype was also more prevalent in patients compared to control group(38.48%and 30.12%respectively,P<0.021).Logistic regression analysis revealed that only one polymorphism(rs1051690 in INSR gene)was associated with increased risk of GC.Carriers of CT genotype had higher odds of GC when compared to CC genotype(OR=1.45,95%PI:1.08-1.95,P=0.01).Similar association was observed in a dominant model for INSR gene,where comparison of TT+CT vs CC genotypes showed an increased risk of GC(OR=1.44,95%PI:1.08-1.90,P=0.01).Other analyzed SNPs were not associated with the presence of GC.CONCLUSION INSR rs1051690 SNP is associated with increased risk of GC,while polymorphisms in IL12B,CCND1 and IL10genes are not linked with the presence of GC.

Gene expression profiles in peripheral blood mononuclear cells of SARS patients

AIM： To investigate the role of inflammatory and anti-vira genes in the pathogenesis of SARS. METHODS： cDNA microarrays were used to screen the gene expression profiles of peripheral blood mononuclear cells （PBMCs） in two SARS patients （one in the acute severe phase and the other in the convalescent phase） and a healthy donor. In addition, real-time qualitative PCR was also performed to verify the reproducibility of the microarray results. The data were further analyzed. RESULTS： Many inflammatory and anti-viral genes were differentially expressed in SARS patients. Compared to the healthy control or the convalescent case, plenty of pro-inflammatory cytokines such as IL-1, TNF-α, IL-8, and MAPK signaling pathway were significantly upregulated in the acute severe case. However, anti-inflammatory agents such as IL-4 receptor, IL-13 receptor, IL-1Ra, and TNF-α-induced proteins 3 and 6 also increased dramatically in the acute severe case. On the contrary, a lot of IFN-stimulated genes like PKR, GBP-1 and 2, CXCL-10 and 11, and JAK/STAT signal pathway were downregulated in the acute severe case compared to the convalescent case. CONCLUSION： Gene expression in SAPS patients mirrors a host state of inflammation and anti-viral immunity at the transcription level, and understanding of gene expression profiles may make contribution to further studies of the SAPS pathogenesis.

Impact of IL28B and OAS gene family polymorphisms on interferon treatment response in Caucasian children chronically infected with hepatitis B virus

AIM To investigate the impact of IL28 B and OAS gene polymorphisms on interferon treatment responses in children with chronic hepatitis B.METHODS We enrolled 52 children(between the ages of 4 and 18) with hepatitis B e antigen-negative chronic hepatitis B(CHB), who were treated with pegylated interferon alfa for 48 wk. Single nucleotide polymorphisms in the OAS1(rs1131476), OAS2(rs1293747),OAS3(rs2072136), OASL(rs10849829) and IL28B(rs12979860, rs12980275 and rs8099917) genes were studied to examine their associations with responses to IFN treatment in paediatric patients. We adopted two criteria for the therapeutic response, achieving an hepatitis B virus(HBV) DNA level < 2000 IU/m L and normalization of ALT activity(< 40 IU/L). To perform the analyses, we compared the patients in terms of achieving a partial response(PR) and a complete response(CR) upon measurement at the 24-wk posttreatment follow-up. RESULTS The PR and CR rates were 80.8% and 42.3%, respectively. Factors such as age, gender and liver histology had no impact on the type of response(partial or complete). A statistically significant relationship between higher baseline HBV DNA and ALT activity levels and lower rates of PR and CR was shown(P < 0.05). The allele association analysis revealed that only the IL-28 B rs12979860(C vs T) and IL28 B rs12980275(A vs G) markers significantly affected the achievement of PR(P = 0.021, OR = 3.3, 95%CI: 1.2-9.2 and P = 0.014, OR = 3.7, 95%CI: 1.3-10.1, respectively). However, in the genotype analysis, only IL-28 B rs12980275 was significantly associated with PR(AA vs AG-GG, P = 0.014, OR = 10.9, 95%CI: 1.3-93.9). The association analysis for CR showed that the TT genotype of IL28 B rs12979860 was present only in the no-CR group(P = 0.033) and the AA genotype of OASL rs10849829 was significantly more frequent in the noCR group(P = 0.044, OR = 0.26, 95%CI: 0.07-0.88). The haplotype analysis revealed significant associations between PR and CR and OAS haplotype(P = 0.0002 and P = 0.001, respectively), but no association with IL28 B haplotype was observed.CONCLUSION IL28 B and OAS polymorphisms are associated with different clinical outcomes in CHB children treated with interferon.

Extensive genetic divergence among Diptychus maculatus populations in northwest China

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province, China. Ill River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure ofD. maculates, four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations, only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined, the haplotype diversity （h） was 0.904 8±0.012 6, nucleotide diversity （n） was 0.027 9±0.013 9, and the average number of pairwise nucleotide differences （k） was 15.878 3±7.139 1. The analysis of molecular variance （AMOVA） showed that 86.31% of the total genetic variation was apportioned among populations, and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. Fst statistical test indicated that all populations were significantly divergent from each other （P〈0.01）. The largest Fst value was between Yurungkash River population and Muzat River population, while the smallest fst value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.

Topological Properties of Complex Networks with Different Types of Nodes

Nodes play different roles or have different functions in many natural and social networks.In this paper,a simple model with different types of nodes and deterministic selective linking rule is proposed.The structural properties by theoretical predictions are investigated that the given model exhibits a power-law distribution.

Virological response to adefovir monotherapy and the risk of adefovir resistance

AIM:To evaluate virological response to adefovir(ADV) monotherapy and emergence of ADV-resistant mutations in lamivudine(LAM)-resistant chronic hepatitis B patients.METHODS:Seventy-seven patients with documented LAM resistance who were treated with 10 mg/d ADV for>96 wk were analyzed for ADV resistance.RESULTS:At week 48 and 96,eight(10%)and 14(18%)of 77 LAM-resistant patients developed the ADV-resistant strain(rtA181V/T and/or rtN236T mutations),respectively.Hepatitis B virus(HBV)DNA levels during therapy were significantly higher in patients who developed ADV resistance than in those who did not.Incidence of ADV resistance at week 96 was 11%,8%and 6%among patients with complete virological response(HBV DNA level<60 IU/mL);0%,5%and 19%among patients with partial virological response(HBV DNA level≥60 to 2000 IU/mL);and 32%,34% and 33%among patients with inadequate virological response(HBV DNA levels>2000 IU/mL)at week 12,week 24 and week 48,respectively.HBV DNA levels >2000 IU/mL at week 24 showed best performance characteristics in predicting ADV resistance.CONCLUSION:Development of ADV resistance mutations was associated with HBV DNA levels,which could identify patients with LAM resistance who are likely to respond to ADV monotherapy.

Coexistence of a Strongly Coupled Prey-predator Model for Holling's Type Ⅲ

In this paper, the two-species prey-predator Lotka-Volterra model with the Holling＇s type III is discussed. By the method of coupled upper and lower solutions and its associated monotone iterations, the existence of solutions for a strongly coupled elliptic system with homogeneous of Dirchlet boundary conditions is derived. These results show that this model admits at least one coexistence state if across-diffusions are weak.

Drift Intermittent Synchronization and Controllability in a Simple Model

A simple model of three coupled oscillators as an approximation of main modes behaviors in a spatial extended system is proposed. Multi-looping generalized synchronization and drift intermittent lag phase synchronization phenomena are found in this simple model. For a certain of parameters in which chaotic-like intermittent behavior exhibit the amplitudes and phases of three modes are controlled to be synchronized states via coupling them with an external periodic mode.

A Remark on Global Existence in H^4 for a Nonlinear Thermoviscoelasticity Equations with Non-monotone Pressure

We consider a one-dimensional continuous model of nutron star, described by a compressible thermoviscoelastic system with a non-monotone equation of state, due to the effective Skyrme nuclear interaction between particles. We will prove that, despite a possible destabilizing influence of the pressure, which is non-monotone and not always positive, the presence of viscosity and a sufficient thermal dissipation describe the global existence of solutions in H^4 with a mixed free boundary problem for our model.

滇西南四个自然保护区鱼类多样性及评价指标探究

为了解滇西南怒江水系的南滚河自然保护区、南捧河自然保护区、永德大雪山自然保护区及澜沧江水系的澜沧江自然保护区鱼类多样性和变化趋势,探讨其差异和变化的原因,本文采用β多样性指数分析了4个保护区的鱼类多样性,并比较了鱼类分类阶元的特有性、单型性和古老成分的有无等多项指标。结果显示,4个自然保护区共有土著鱼类85种,隶属于6目13科45属。在中国仅见于怒江水系的4个特有属有异鲴属(Aspidoparia)和新条鳅属(Neonoemacheilus)分布于这3个保护区中;18种特有种中,仅分布在这3个保护区的狭域特有种5种。在中国仅分布于澜沧江水系的属有31个,但仅安巴沙鳅属(Ambastaia)分布于澜沧江保护区;在澜沧江保护区分布着中国仅见于澜沧江水系的特有种20种,其中狭域特有种3种。怒江水系的3个保护区分布有1个单型属,即鳗鲡属(Anguilla),但没有单型种;澜沧江自然保护区无单型属与单型种分布。4个保护区中的鱼类均系晚第三纪和第四纪形成的种类或类群,没有古老或孑遗种类。β多样性结果显示,在4个保护区中澜沧江自然保护区的鱼类多样性最丰富,而南滚河自然保护区的丰富程度最低,但是怒江水系3个自然保护区鱼类多样性的代表性及保护地位比澜沧江自然保护区的要高。而特有阶元和单型性阶元的存在体现出怒江水系3个自然保护区的保护价值及保护意义比澜沧江保护区高。地理范围跨度大小、生境空间异质性高低、保护区面积大小及支流多少等是影响鱼类多样性的主要因素。因此,规划和设计保护区时,如果能在水系的上、中、下游分别规划1条一级支流作为保护区,可使该水系的绝大多数鱼类得到保护。