Si based single electron transistor (SET) is fabricated successfully on p type SIMOX substrate,based on electron beam (EB) lithography,reactive ion etching (RIE) and thermal oxidation.In particular,using thermal oxi...Si based single electron transistor (SET) is fabricated successfully on p type SIMOX substrate,based on electron beam (EB) lithography,reactive ion etching (RIE) and thermal oxidation.In particular,using thermal oxidation and etching off the oxide layer,a one dimensional Si quantum wire can be converted into several quantum dots inside quantum wire in connection with the source and drain regions.The differential conductance (d I ds /d V ds ) oscillations and the Coulomb staircases in the source drain current ( I ds ) are shown clearly dependent on the source drain voltage at 5 3K.The I ds V gs (gate voltage) oscillations are observed from the I ds V gs characteristics as a function of V gs at different temperatures and various values of V ds .For a SET whose total capacitance is about 9 16aF,the I ds V gs oscillations can be observed at 77K.展开更多
[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16(45) orthogonal experimental design was adopted for optimization of five important fac...[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16(45) orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2+ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2+, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.展开更多
To solve the query processing correctness problem for semantic-based relational data integration,the semantics of SAPRQL(simple protocol and RDF query language) queries is defined.In the course of query rewriting,al...To solve the query processing correctness problem for semantic-based relational data integration,the semantics of SAPRQL(simple protocol and RDF query language) queries is defined.In the course of query rewriting,all relative tables are found and decomposed into minimal connectable units.Minimal connectable units are joined according to semantic queries to produce the semantically correct query plans.Algorithms for query rewriting and transforming are presented.Computational complexity of the algorithms is discussed.Under the worst case,the query decomposing algorithm can be finished in O(n2) time and the query rewriting algorithm requires O(nm) time.And the performance of the algorithms is verified by experiments,and experimental results show that when the length of query is less than 8,the query processing algorithms can provide satisfactory performance.展开更多
Based on the orthodox theory,a model of a single electron transistor (SET) of metallic tunneling junctions is built using the master equation method. Several parameters of the device, such as capacitance, resistance...Based on the orthodox theory,a model of a single electron transistor (SET) of metallic tunneling junctions is built using the master equation method. Several parameters of the device, such as capacitance, resistance and temperature,are input into the model and thus the I-V curves are attained. These curves are consistent with those from other experiments; therefore, the model is verified. However, there still exists a difference between simulated results and experimental results,mainly comes from the stationary case of the master equation. In other words, precision of simulated results would be increased if the transient case of the master equation is considered. Moreover, the current increases exponentially at higher drain voltages, which is due to the fact that the barrier suppression is caused by the image charge potential.展开更多
Objective To identify epitope relating to BAC 5 mcAb, a kind of monoclonal antibody (mcAb) located on the surface of nasopharyngeal carcinoma (NPC) cells. Methods Using BAC 5 mcAb as a selected target, the 3 rou...Objective To identify epitope relating to BAC 5 mcAb, a kind of monoclonal antibody (mcAb) located on the surface of nasopharyngeal carcinoma (NPC) cells. Methods Using BAC 5 mcAb as a selected target, the 3 rounds of biopanning to a 12 mer random peptide library (RPL) presented by M13 phages were carried out. The positive M13 phage clones were chosen and confirmed with sandwich ELISA for antibody capture and competitive assay. The exogenous DNA fragments in the positive/negative M13 phages were sequenced to deduce and compare the order of the amino acids of exogenous peptides among the phage clones. Results 77% (35/45) of the phages eluted from the 3rd round of biopnning could be captured by BAC 5 mcAb. The 3 kinds of the peptides were displayed by M13 phages from the 8 positive clones identified with competitive assay. The same character of '-P-V-'structure existed near N-terminus of the 3 different peptides, i.e. -H-Q-S-H-Y-P-Y-P-V-V-S-L- (4/8) -Q-N-Q-A-W-F-S-Q-P-V-R-M- (3/8) and T-Q-A-Y-K-G-F-P-V-L-P-S- (1/8) in comparison with the peptide ' -N-H-Q-S-T-F-W-Q-K-W-T-A-' displayed by M13 phages from the negative clones (6/6). Conclusion BAC 5 mcAb can recognize the 3 kinds of the peptides with-P-V-structure near N-terminus. These peptides mimic the structure of the epitope on the surface of NPC cells recognized by BAC 5 mcAb.展开更多
A library of 2 ×107 random octapeptides was constructed by use of phagemid-based monovalent phage display system. The randomly synthesized degenerated oligodeoxyribonucleotides (oligos ) were fused to the truncat...A library of 2 ×107 random octapeptides was constructed by use of phagemid-based monovalent phage display system. The randomly synthesized degenerated oligodeoxyribonucleotides (oligos ) were fused to the truncated g Ⅲ (p230-p403). Sequence analysis of 11 randomly chosen clones suggested that the degenerated inserts and its deduced amino acid (aa) sequences are randomly distributed. The library was used to select binding peptides to the monoclonal antibody (mAb) 9E10, which recognizes a continuous decapeptide epi- tope of denatured human c-myc protein. After four to five rounds of panning, most of the eluted clones could bind to 9E10. Sequence analysis of the selected positive clones indicated that the binding sequences could fall into two classes, one class (clone 1) shares a consensus motif, ISE x x L, with c-myc decapeptide; and the sequences of the other class are entirely different. The binding of both classes to 9E10 could be specifically inhibited by free c-myc decapeptide. The immunogenicity of the phage peptide was further investigated by construction of multivalent displayed phage peptides and immunization of animals with or without adjuvant. ELISA and competitive ELISA showed that anti-serum from both mice and rabbit immunized with either clone could bind to the original antigen, c-myc decapeptide. These results denote that in spite of the dissimilarity of the selected peptides with c-myc decapeptide, they are capable of inducing similar immune respones in vivo, thus actually mimicking the antigen epitope.展开更多
A new web product data management architecture is presented. The three-tier web architecture and Simple Object Access Protocol (SOAP) are combined to build the web-based product data management (PDM) system which incl...A new web product data management architecture is presented. The three-tier web architecture and Simple Object Access Protocol (SOAP) are combined to build the web-based product data management (PDM) system which includes three tiers: the user services tier, the business services tier, and the data services tier. The client service component uses the server-side technology, and Extensible Markup Language (XML) web service which uses SOAP as the communication protocol is chosen as the business service component. To illustrate how to build a web-based PDM system using the proposed architecture, a case PDM system which included three logical tires was built. To use the security and central management features of the database, a stored procedure was recommended in the data services tier. The business object was implemented as an XML web service so that client could use standard internet protocols to communicate with the business object from any platform. In order to satisfy users using all sorts of browser, the server-side technology and Microsoft ASP.NET was used to create the dynamic user interface.展开更多
Damage in rock salt has significant implication on permeability, which affects the tightness of underground salt cavern gas storage in further. During the leaching of a salt cavern, the brine with formation temperatur...Damage in rock salt has significant implication on permeability, which affects the tightness of underground salt cavern gas storage in further. During the leaching of a salt cavern, the brine with formation temperature and pressure can promote the self-healing of rock salt in the excavation damage zone (EDZ). Laboratory tests were conducted to study the promoting effect. The permeability of two intact rock salt specimens was tested. Then they were damaged into two kinds of the state respectively through uniaxial compression. After that, they were put in saturated brine (with a temperature of 50℃ and pressure of 12 MPa, which we called the repair environment in this paper) for 7 d. Finally, the permeability and mechanical properties were obtained after the damaged specimens being repaired. The results show that the permeability of intact rock salt is below 10^-19 m^2;the permeability increases by more than two orders because of damage;the permeability decreases significantly after being repaired, which can be comparable to its intact state. Discussions of the repair mechanisms are presented (especially the mechanism of recrystallization), which may help to provide significant guidance for the study of the tightness and stability of gas storage facilities in China.展开更多
The problem of maintaining data consistency in mobile broadcast environments is researched. Quasi serializability is formally defined and analyzed at first. It was shown that quasi serializability is less stringent th...The problem of maintaining data consistency in mobile broadcast environments is researched. Quasi serializability is formally defined and analyzed at first. It was shown that quasi serializability is less stringent than serializability when database consistency is maintained for transactions. Then, corresponding concurrency control protocol that supports both update transactions and read-only transactions is outlined for mobile broadcast environments. Finally, the simulation results confirmed that the proposed protocol could improve the response time significantly.展开更多
Uniaxial compression experiments on horizontal and ervoir were conducted at different temperatures and strain rates vertical samples of first-year freshwater ice in a res- with an electronic universal machine equipped...Uniaxial compression experiments on horizontal and ervoir were conducted at different temperatures and strain rates vertical samples of first-year freshwater ice in a res- with an electronic universal machine equipped with low temperature cabinet. The results show that there is no difference between the strengths of two horizontal samples with grain sizes ranging from 1 to 4 mm and 4 to 14 mm, while the strength of the 1--4 mm vertical samples is 1.4 times higher than that of the 4--14 mm vertical samples because of the change of crystal structure. For different load- ing directions, the strengths of the horizontal samples do not differ from those of the vertical samples with the same grain sizes. The relation among the uniaxial compressive strength, strain rate and temperature was established through data analysis in both the ductile and brittle regions.展开更多
Prostate-specific membrane antigen (PSMA) is a cellsurface glycoprotein expressed predominantly in prostatesecretory acinar epithelium and prostate cancer cells aswell as in several extraprostatic tissues. Mouse monoc...Prostate-specific membrane antigen (PSMA) is a cellsurface glycoprotein expressed predominantly in prostatesecretory acinar epithelium and prostate cancer cells aswell as in several extraprostatic tissues. Mouse monoclonal antibody 4G5 specific to the extracellular domainof PSMA was used to screen two phage displayed peptide libraries (9aa linear and 9aa cys library). Three 4G5reactive phagotopes were identified. Sequence analysis ofisolated clones demonstrated that the interaction motif'VDPA/SK' has high homology to 719-725aa on PSMA.Immunohistochemical staming of the prostate cancer sam ple with the PSMA-mimic phagotope (mimotope) immunized serum antibodies demonstrate that the mimotopeisolated from the phage displayed peptide libraries can induce PSMA specific immune response in vivo.展开更多
Matching soil grid unit resolutions with polygon unit map scales is important to minimize the uncertainty of regional soil organic carbon(SOC) pool simulation due to their strong influences on the modeling.A series of...Matching soil grid unit resolutions with polygon unit map scales is important to minimize the uncertainty of regional soil organic carbon(SOC) pool simulation due to their strong influences on the modeling.A series of soil grid units at varying cell sizes was derived from soil polygon units at six map scales,namely,1:50 000(C5),1:200 000(D2),1:500 000(P5),1:1 000 000(N1),1:4 000 000(N4) and 1:14 000 000(N14),in the Taihu Region of China.Both soil unit formats were used for regional SOC pool simulation with a De Nitrification-DeC omposition(DNDC) process-based model,which spans the time period from 1982 to 2000 at the six map scales.Four indices,namely,soil type number(STN),area(AREA),average SOC density(ASOCD) and total SOC stocks(SOCS) of surface paddy soils that were simulated by the DNDC,were distinguished from all these soil polygon and grid units.Subjecting to the four index values(IV) from the parent polygon units,the variations in an index value(VIV,%) from the grid units were used to assess its dataset accuracy and redundancy,which reflects the uncertainty in the simulation of SOC pools.Optimal soil grid unit resolutions were generated and suggested for the DNDC simulation of regional SOC pools,matching their respective soil polygon unit map scales.With these optimal raster resolutions,the soil grid units datasets can have the same accuracy as their parent polygon units datasets without any redundancy,when VIV < 1% was assumed to be a criterion for all four indices.A quadratic curve regression model,namely,y = – 0.80 × 10^(–6)x^2 + 0.0228 x + 0.0211(R^2 = 0.9994,P < 0.05),and a power function model R? = 10.394?^(0.2153)(R^2 = 0.9759,P < 0.05) were revealed,which describe the relationship between the optimal soil grid unit resolution(y,km) and soil polygon unit map scale(1:10 000x),the ratio(R?,%) of the optimal soil grid size to average polygon patch size(?,km^2) and the ?,with the highest R^2 among different mathematical regressions,respectively.This knowledge may facilitate the grid partitioning of regions during the investigation and simulation of SOC pool dynamics at a certain map scale,and be referenced to other landscape polygon patches' mesh partition.展开更多
文摘Si based single electron transistor (SET) is fabricated successfully on p type SIMOX substrate,based on electron beam (EB) lithography,reactive ion etching (RIE) and thermal oxidation.In particular,using thermal oxidation and etching off the oxide layer,a one dimensional Si quantum wire can be converted into several quantum dots inside quantum wire in connection with the source and drain regions.The differential conductance (d I ds /d V ds ) oscillations and the Coulomb staircases in the source drain current ( I ds ) are shown clearly dependent on the source drain voltage at 5 3K.The I ds V gs (gate voltage) oscillations are observed from the I ds V gs characteristics as a function of V gs at different temperatures and various values of V ds .For a SET whose total capacitance is about 9 16aF,the I ds V gs oscillations can be observed at 77K.
基金Supported by Central University Basic Research Operating Expenses Special Fund(XDJK2011C026)Southwest University Doctoral Fund(09BSR04)~~
文摘[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16(45) orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2+ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2+, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.
基金Weaponry Equipment Pre-Research Foundation of PLA Equipment Ministry (No. 9140A06050409JB8102)Pre-Research Foundation of PLA University of Science and Technology (No. 2009JSJ11)
文摘To solve the query processing correctness problem for semantic-based relational data integration,the semantics of SAPRQL(simple protocol and RDF query language) queries is defined.In the course of query rewriting,all relative tables are found and decomposed into minimal connectable units.Minimal connectable units are joined according to semantic queries to produce the semantically correct query plans.Algorithms for query rewriting and transforming are presented.Computational complexity of the algorithms is discussed.Under the worst case,the query decomposing algorithm can be finished in O(n2) time and the query rewriting algorithm requires O(nm) time.And the performance of the algorithms is verified by experiments,and experimental results show that when the length of query is less than 8,the query processing algorithms can provide satisfactory performance.
文摘Based on the orthodox theory,a model of a single electron transistor (SET) of metallic tunneling junctions is built using the master equation method. Several parameters of the device, such as capacitance, resistance and temperature,are input into the model and thus the I-V curves are attained. These curves are consistent with those from other experiments; therefore, the model is verified. However, there still exists a difference between simulated results and experimental results,mainly comes from the stationary case of the master equation. In other words, precision of simulated results would be increased if the transient case of the master equation is considered. Moreover, the current increases exponentially at higher drain voltages, which is due to the fact that the barrier suppression is caused by the image charge potential.
文摘Objective To identify epitope relating to BAC 5 mcAb, a kind of monoclonal antibody (mcAb) located on the surface of nasopharyngeal carcinoma (NPC) cells. Methods Using BAC 5 mcAb as a selected target, the 3 rounds of biopanning to a 12 mer random peptide library (RPL) presented by M13 phages were carried out. The positive M13 phage clones were chosen and confirmed with sandwich ELISA for antibody capture and competitive assay. The exogenous DNA fragments in the positive/negative M13 phages were sequenced to deduce and compare the order of the amino acids of exogenous peptides among the phage clones. Results 77% (35/45) of the phages eluted from the 3rd round of biopnning could be captured by BAC 5 mcAb. The 3 kinds of the peptides were displayed by M13 phages from the 8 positive clones identified with competitive assay. The same character of '-P-V-'structure existed near N-terminus of the 3 different peptides, i.e. -H-Q-S-H-Y-P-Y-P-V-V-S-L- (4/8) -Q-N-Q-A-W-F-S-Q-P-V-R-M- (3/8) and T-Q-A-Y-K-G-F-P-V-L-P-S- (1/8) in comparison with the peptide ' -N-H-Q-S-T-F-W-Q-K-W-T-A-' displayed by M13 phages from the negative clones (6/6). Conclusion BAC 5 mcAb can recognize the 3 kinds of the peptides with-P-V-structure near N-terminus. These peptides mimic the structure of the epitope on the surface of NPC cells recognized by BAC 5 mcAb.
文摘A library of 2 ×107 random octapeptides was constructed by use of phagemid-based monovalent phage display system. The randomly synthesized degenerated oligodeoxyribonucleotides (oligos ) were fused to the truncated g Ⅲ (p230-p403). Sequence analysis of 11 randomly chosen clones suggested that the degenerated inserts and its deduced amino acid (aa) sequences are randomly distributed. The library was used to select binding peptides to the monoclonal antibody (mAb) 9E10, which recognizes a continuous decapeptide epi- tope of denatured human c-myc protein. After four to five rounds of panning, most of the eluted clones could bind to 9E10. Sequence analysis of the selected positive clones indicated that the binding sequences could fall into two classes, one class (clone 1) shares a consensus motif, ISE x x L, with c-myc decapeptide; and the sequences of the other class are entirely different. The binding of both classes to 9E10 could be specifically inhibited by free c-myc decapeptide. The immunogenicity of the phage peptide was further investigated by construction of multivalent displayed phage peptides and immunization of animals with or without adjuvant. ELISA and competitive ELISA showed that anti-serum from both mice and rabbit immunized with either clone could bind to the original antigen, c-myc decapeptide. These results denote that in spite of the dissimilarity of the selected peptides with c-myc decapeptide, they are capable of inducing similar immune respones in vivo, thus actually mimicking the antigen epitope.
基金the National Key Project Foundation of China (No. 2001BA201A0605) and partially supported by the State Key Lab for Mechanical Transmission..
文摘A new web product data management architecture is presented. The three-tier web architecture and Simple Object Access Protocol (SOAP) are combined to build the web-based product data management (PDM) system which includes three tiers: the user services tier, the business services tier, and the data services tier. The client service component uses the server-side technology, and Extensible Markup Language (XML) web service which uses SOAP as the communication protocol is chosen as the business service component. To illustrate how to build a web-based PDM system using the proposed architecture, a case PDM system which included three logical tires was built. To use the security and central management features of the database, a stored procedure was recommended in the data services tier. The business object was implemented as an XML web service so that client could use standard internet protocols to communicate with the business object from any platform. In order to satisfy users using all sorts of browser, the server-side technology and Microsoft ASP.NET was used to create the dynamic user interface.
基金Project(201704910741) supported by the China Scholarship CouncilProjects(51874274,51774266,51874273,51621006) supported by the National Natural Science Foundation of ChinaProject(2018YFC0808401) supported by the National Key Research and Development Program of China
文摘Damage in rock salt has significant implication on permeability, which affects the tightness of underground salt cavern gas storage in further. During the leaching of a salt cavern, the brine with formation temperature and pressure can promote the self-healing of rock salt in the excavation damage zone (EDZ). Laboratory tests were conducted to study the promoting effect. The permeability of two intact rock salt specimens was tested. Then they were damaged into two kinds of the state respectively through uniaxial compression. After that, they were put in saturated brine (with a temperature of 50℃ and pressure of 12 MPa, which we called the repair environment in this paper) for 7 d. Finally, the permeability and mechanical properties were obtained after the damaged specimens being repaired. The results show that the permeability of intact rock salt is below 10^-19 m^2;the permeability increases by more than two orders because of damage;the permeability decreases significantly after being repaired, which can be comparable to its intact state. Discussions of the repair mechanisms are presented (especially the mechanism of recrystallization), which may help to provide significant guidance for the study of the tightness and stability of gas storage facilities in China.
文摘The problem of maintaining data consistency in mobile broadcast environments is researched. Quasi serializability is formally defined and analyzed at first. It was shown that quasi serializability is less stringent than serializability when database consistency is maintained for transactions. Then, corresponding concurrency control protocol that supports both update transactions and read-only transactions is outlined for mobile broadcast environments. Finally, the simulation results confirmed that the proposed protocol could improve the response time significantly.
基金Supported by National Natural Science Foundation of China (No. 50879008 and No. 51079021)
文摘Uniaxial compression experiments on horizontal and ervoir were conducted at different temperatures and strain rates vertical samples of first-year freshwater ice in a res- with an electronic universal machine equipped with low temperature cabinet. The results show that there is no difference between the strengths of two horizontal samples with grain sizes ranging from 1 to 4 mm and 4 to 14 mm, while the strength of the 1--4 mm vertical samples is 1.4 times higher than that of the 4--14 mm vertical samples because of the change of crystal structure. For different load- ing directions, the strengths of the horizontal samples do not differ from those of the vertical samples with the same grain sizes. The relation among the uniaxial compressive strength, strain rate and temperature was established through data analysis in both the ductile and brittle regions.
文摘Prostate-specific membrane antigen (PSMA) is a cellsurface glycoprotein expressed predominantly in prostatesecretory acinar epithelium and prostate cancer cells aswell as in several extraprostatic tissues. Mouse monoclonal antibody 4G5 specific to the extracellular domainof PSMA was used to screen two phage displayed peptide libraries (9aa linear and 9aa cys library). Three 4G5reactive phagotopes were identified. Sequence analysis ofisolated clones demonstrated that the interaction motif'VDPA/SK' has high homology to 719-725aa on PSMA.Immunohistochemical staming of the prostate cancer sam ple with the PSMA-mimic phagotope (mimotope) immunized serum antibodies demonstrate that the mimotopeisolated from the phage displayed peptide libraries can induce PSMA specific immune response in vivo.
基金Under the auspices of Special Project of National Key Research and Development Program(No.2016YFD0200301)National Natural Science Foundation of China(No.41571206)Special Project of National Science and Technology Basic Work(No.2015FY110700-S2)
文摘Matching soil grid unit resolutions with polygon unit map scales is important to minimize the uncertainty of regional soil organic carbon(SOC) pool simulation due to their strong influences on the modeling.A series of soil grid units at varying cell sizes was derived from soil polygon units at six map scales,namely,1:50 000(C5),1:200 000(D2),1:500 000(P5),1:1 000 000(N1),1:4 000 000(N4) and 1:14 000 000(N14),in the Taihu Region of China.Both soil unit formats were used for regional SOC pool simulation with a De Nitrification-DeC omposition(DNDC) process-based model,which spans the time period from 1982 to 2000 at the six map scales.Four indices,namely,soil type number(STN),area(AREA),average SOC density(ASOCD) and total SOC stocks(SOCS) of surface paddy soils that were simulated by the DNDC,were distinguished from all these soil polygon and grid units.Subjecting to the four index values(IV) from the parent polygon units,the variations in an index value(VIV,%) from the grid units were used to assess its dataset accuracy and redundancy,which reflects the uncertainty in the simulation of SOC pools.Optimal soil grid unit resolutions were generated and suggested for the DNDC simulation of regional SOC pools,matching their respective soil polygon unit map scales.With these optimal raster resolutions,the soil grid units datasets can have the same accuracy as their parent polygon units datasets without any redundancy,when VIV < 1% was assumed to be a criterion for all four indices.A quadratic curve regression model,namely,y = – 0.80 × 10^(–6)x^2 + 0.0228 x + 0.0211(R^2 = 0.9994,P < 0.05),and a power function model R? = 10.394?^(0.2153)(R^2 = 0.9759,P < 0.05) were revealed,which describe the relationship between the optimal soil grid unit resolution(y,km) and soil polygon unit map scale(1:10 000x),the ratio(R?,%) of the optimal soil grid size to average polygon patch size(?,km^2) and the ?,with the highest R^2 among different mathematical regressions,respectively.This knowledge may facilitate the grid partitioning of regions during the investigation and simulation of SOC pool dynamics at a certain map scale,and be referenced to other landscape polygon patches' mesh partition.