Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this ...Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.展开更多
The effect of the repeated unidirectional bending （RUB） process and annealing on the formability of magnesium alloy sheets was investigated. The RUB process and annealing treatments produce two effects on microstruc...The effect of the repeated unidirectional bending （RUB） process and annealing on the formability of magnesium alloy sheets was investigated. The RUB process and annealing treatments produce two effects on microstructure： grain coarsening and weakening of the texture. The sheet that underwent RUB and was annealed at 300 ℃exhibits the best formability owing to the reduction of the （0002） basal texture intensity, which results in low yield strength, large fracture elongation, small Lankford value （r-value） and large strain hardening exponent （n-value）. Compared with the as-received sheet, the coarse-grain sheet produced by RUB and annealing at 400 ℃ exhibits lower tensile properties but higher formability. The phenomenon is because the deformation twin enhanced by grain coarsening can accommodate the strain of thickness.展开更多
The stress aging behavior of Al-Cu alloy under various applied stresses, i.e., elastic stress, yield stress and plasticdeformation stress, was investigated using single crystals. The resulting microstructures and the ...The stress aging behavior of Al-Cu alloy under various applied stresses, i.e., elastic stress, yield stress and plasticdeformation stress, was investigated using single crystals. The resulting microstructures and the yield strength were examined bytransmission electron microscopy （TEM） and compression tests, respectively. The results indicate that an elastic stress of 15 MPa ishigh enough to influence the precipitation distribution of θ′ during aging at 180℃. The applied stress loading along [116]Aldirection results in increased number density of θ′ on （001）Al habit planes. This result becomes more significant with increasingapplied stress and leads to lower yield strength of Al-Cu single crystals during aging. Moreover, the generation of the preferentialorientation of θ′ was discussed by the effect of the dislocation induced by applied stress as well as the role of the misfit between theθ′-precipitate and Al matrix. The results are in agreement with the effect of the latter one.展开更多
DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone pro...DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone proteins, we reconstitute nucleosomes using lambda DNA and whole histone proteins by dialysis and perform direct atomic force microscopy （AFM） imaging. Compared with non-specific DNA and histone binding, nucleosomes are formed within the assembled “beads-on-a-string” nucleosomal array by dialysis. These observations facilitate the establishment of the molecular mechanisms of nucleosome and demonstrate the capability of AFM for protein-DNA interaction analysis.展开更多
Two experimental single crystal superalloys with 2% Cr and 4% Cr (mass fraction) were cast in a directionally solidified furnace, while other alloying element contents were kept unchanged. The effects of Cr content on...Two experimental single crystal superalloys with 2% Cr and 4% Cr (mass fraction) were cast in a directionally solidified furnace, while other alloying element contents were kept unchanged. The effects of Cr content on the microstructure, phase stability, tensile properties at 1100 °C and stress rupture properties at 1070 °C and 160 MPa of the single crystal superalloy were investigated. The results show that the size ofγ′ phase particles become small and uniform, and the cubic shape turns a little regular with the increase of Cr content. Theγ′ directional coarsening and rafting were observed in the 2% Cr and 4% Cr alloys after long term aging (LTA) at 1100 °C. The rafting rate ofγ′ phase increased with increasing Cr content. Needle-shaped topologically close packed (TCP) phases precipitated and grew along fixed direction in both alloys. The precipitating rate and volume fraction of TCP phases significantly increased with the increase of Cr content. The tensile property of the alloy increased and the stress rupture properties of the alloy decreased with the increase of Cr content at high temperature. The increase of Cr content increased the partition ratio of TCP forming elements, Re, W, and Mo, and the saturation degrees of these elements inγ phases increased. Therefore, the high temperature phase stability of the alloy decreased with the increase of Cr content.展开更多
[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription（DDRT）analysis.[Method] Soybean varieties ＂Jilin 30＂ and ＂Tongnong 13＂ were used as materials to carry ...[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription（DDRT）analysis.[Method] Soybean varieties ＂Jilin 30＂ and ＂Tongnong 13＂ were used as materials to carry out analysis on false positives in DDRT analysis.[Result] An important origin of false positives appeared in DDRT analysis was the non-specific amplification caused by the combination of single primer and cDNA.The parallel PCR test of single primer should be set so as to verify whether the obtained fragments were the false positives or the PCR productions combined with single primer.[Conclusion] This study had provided basis for improving the success rate of DDRT experiment.展开更多
Contacting mode atomic force microscopy (AFM) is used to measure the In 0.35 Ga 0.65 As/GaAs epilayer grown at low temperature (460℃).Unlike the normal layer by layer growth (FvdM mode) or self organized i...Contacting mode atomic force microscopy (AFM) is used to measure the In 0.35 Ga 0.65 As/GaAs epilayer grown at low temperature (460℃).Unlike the normal layer by layer growth (FvdM mode) or self organized islands growth (SK mode),samples grown under 460℃ are found to be large islands with atomic thick terraces.AFM measurements reveale near one monolayer high steps.This kind of growth is good between FvdM and SK growth modes and can be used to understand the evolution of strained epitaxy from FvdM to SK mode.展开更多
Laser multi\|layer cladding experiments were performed on the substrate of DD3 single crystal with FGH95 powder as cladding material.The solidification microstructure in the sample was investigated.It was found that t...Laser multi\|layer cladding experiments were performed on the substrate of DD3 single crystal with FGH95 powder as cladding material.The solidification microstructure in the sample was investigated.It was found that the solidification microstructure was greatly influenced by the crystallography orientation of the substrate and the local solidification conditions.When the angle between the preferred orientation of the single crystal and the direction of heat flow in the cladding layer is less than 30°,single crystal cladding layers were acquired.Otherwise the crystallography orientation of the cladding layer will deviate from the orientation of the substrate and the microstructure with polycrystalline appears.Meanwhile,even when the experiments were performed on the same preferred crystal surface,the solidification microstructures will be different distinctly resulting from the variation of the local solidification conditions.The secondary arms were degenerated and the primary arm spacing was about 10\|20μm.Further investigation shows that the phases of the cladding layer are mainly made up ofγ,γ′,the flower\|likeγ/γ′eutectic and carbide.The morphology ofγ′was cubical and the size is less than 0.1μm.展开更多
基金support from the National Institute of General Medical Sciences(1R35G M147172-01).
文摘Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.
基金Project(CSTC2010AA4035)supported by Scientific and Technological Project of Chongqing Science and Technology Commission,ChinaProject(50504019)supported by the National Natural Science Foundation of China+1 种基金Project(CDJZR11130008)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(CDJXS10130001)supported by the Chongqing University Postgraduates'Science and Innovation Fund,China
文摘The effect of the repeated unidirectional bending （RUB） process and annealing on the formability of magnesium alloy sheets was investigated. The RUB process and annealing treatments produce two effects on microstructure： grain coarsening and weakening of the texture. The sheet that underwent RUB and was annealed at 300 ℃exhibits the best formability owing to the reduction of the （0002） basal texture intensity, which results in low yield strength, large fracture elongation, small Lankford value （r-value） and large strain hardening exponent （n-value）. Compared with the as-received sheet, the coarse-grain sheet produced by RUB and annealing at 400 ℃ exhibits lower tensile properties but higher formability. The phenomenon is because the deformation twin enhanced by grain coarsening can accommodate the strain of thickness.
基金Project(51375503)supported by the National Natural Science Foundation of China
文摘The stress aging behavior of Al-Cu alloy under various applied stresses, i.e., elastic stress, yield stress and plasticdeformation stress, was investigated using single crystals. The resulting microstructures and the yield strength were examined bytransmission electron microscopy （TEM） and compression tests, respectively. The results indicate that an elastic stress of 15 MPa ishigh enough to influence the precipitation distribution of θ′ during aging at 180℃. The applied stress loading along [116]Aldirection results in increased number density of θ′ on （001）Al habit planes. This result becomes more significant with increasingapplied stress and leads to lower yield strength of Al-Cu single crystals during aging. Moreover, the generation of the preferentialorientation of θ′ was discussed by the effect of the dislocation induced by applied stress as well as the role of the misfit between theθ′-precipitate and Al matrix. The results are in agreement with the effect of the latter one.
基金ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China （No.11274374）, the National Basic Research Program of China （No.2009CB930704）, and the Basic Scientific Research Foundation of China Agricultural University （No.2012QJ026）.
文摘DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone proteins, we reconstitute nucleosomes using lambda DNA and whole histone proteins by dialysis and perform direct atomic force microscopy （AFM） imaging. Compared with non-specific DNA and histone binding, nucleosomes are formed within the assembled “beads-on-a-string” nucleosomal array by dialysis. These observations facilitate the establishment of the molecular mechanisms of nucleosome and demonstrate the capability of AFM for protein-DNA interaction analysis.
文摘Two experimental single crystal superalloys with 2% Cr and 4% Cr (mass fraction) were cast in a directionally solidified furnace, while other alloying element contents were kept unchanged. The effects of Cr content on the microstructure, phase stability, tensile properties at 1100 °C and stress rupture properties at 1070 °C and 160 MPa of the single crystal superalloy were investigated. The results show that the size ofγ′ phase particles become small and uniform, and the cubic shape turns a little regular with the increase of Cr content. Theγ′ directional coarsening and rafting were observed in the 2% Cr and 4% Cr alloys after long term aging (LTA) at 1100 °C. The rafting rate ofγ′ phase increased with increasing Cr content. Needle-shaped topologically close packed (TCP) phases precipitated and grew along fixed direction in both alloys. The precipitating rate and volume fraction of TCP phases significantly increased with the increase of Cr content. The tensile property of the alloy increased and the stress rupture properties of the alloy decreased with the increase of Cr content at high temperature. The increase of Cr content increased the partition ratio of TCP forming elements, Re, W, and Mo, and the saturation degrees of these elements inγ phases increased. Therefore, the high temperature phase stability of the alloy decreased with the increase of Cr content.
文摘[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription（DDRT）analysis.[Method] Soybean varieties ＂Jilin 30＂ and ＂Tongnong 13＂ were used as materials to carry out analysis on false positives in DDRT analysis.[Result] An important origin of false positives appeared in DDRT analysis was the non-specific amplification caused by the combination of single primer and cDNA.The parallel PCR test of single primer should be set so as to verify whether the obtained fragments were the false positives or the PCR productions combined with single primer.[Conclusion] This study had provided basis for improving the success rate of DDRT experiment.
文摘Contacting mode atomic force microscopy (AFM) is used to measure the In 0.35 Ga 0.65 As/GaAs epilayer grown at low temperature (460℃).Unlike the normal layer by layer growth (FvdM mode) or self organized islands growth (SK mode),samples grown under 460℃ are found to be large islands with atomic thick terraces.AFM measurements reveale near one monolayer high steps.This kind of growth is good between FvdM and SK growth modes and can be used to understand the evolution of strained epitaxy from FvdM to SK mode.
基金National Key Basic Research Development Program me of china(No.G2000067205-3)
文摘Laser multi\|layer cladding experiments were performed on the substrate of DD3 single crystal with FGH95 powder as cladding material.The solidification microstructure in the sample was investigated.It was found that the solidification microstructure was greatly influenced by the crystallography orientation of the substrate and the local solidification conditions.When the angle between the preferred orientation of the single crystal and the direction of heat flow in the cladding layer is less than 30°,single crystal cladding layers were acquired.Otherwise the crystallography orientation of the cladding layer will deviate from the orientation of the substrate and the microstructure with polycrystalline appears.Meanwhile,even when the experiments were performed on the same preferred crystal surface,the solidification microstructures will be different distinctly resulting from the variation of the local solidification conditions.The secondary arms were degenerated and the primary arm spacing was about 10\|20μm.Further investigation shows that the phases of the cladding layer are mainly made up ofγ,γ′,the flower\|likeγ/γ′eutectic and carbide.The morphology ofγ′was cubical and the size is less than 0.1μm.
