采用高效液相色谱法,以丁烯基苯酞为内参物,建立川芎中1种酚酸类成分和5种苯酞类成分的单标多组分HPLC定量分析方法,并考察和验证该方法在川芎质量评价中应用的可行性和准确性。实验所用流动相为乙腈-0.2%甲酸水溶液,流速为1.0 m L·...采用高效液相色谱法,以丁烯基苯酞为内参物,建立川芎中1种酚酸类成分和5种苯酞类成分的单标多组分HPLC定量分析方法,并考察和验证该方法在川芎质量评价中应用的可行性和准确性。实验所用流动相为乙腈-0.2%甲酸水溶液,流速为1.0 m L·min-1,柱温30℃,检测波长分别为252 nm(阿魏酸、藁本内酯、丁烯基苯酞)和266 nm(洋川芎内酯I、洋川芎内酯A、阿魏酸松柏酯),进样量20μL,梯度洗脱。结果显示,单标多组分HPLC定量分析法计算所得值与外标法实测值之间无显著性差异,RSD值均小于5%;相对校正因子的耐用性较好,色谱条件改变后,相对校正因子变化的RSD值均小于5%。所建立的单标多组分HPLC定量分析法可准确地用于中药川芎的多指标同步质量控制。展开更多
To gain superior computational efficiency, it might be necessary to change the underlying philosophy of the simplex method. In this paper, we propose a Phase-1 method along this line. We relax not only the conventiona...To gain superior computational efficiency, it might be necessary to change the underlying philosophy of the simplex method. In this paper, we propose a Phase-1 method along this line. We relax not only the conventional condition that some function value increases monotonically, but also the condition that all feasible variables remain feasible after basis change in Phase-1. That is, taking a purely combinatorial approach to achieving feasibility. This enables us to get rid of ratio test in pivoting, reducing computational cost per iteration to a large extent. Numerical results on a group of problems are encouraging.展开更多
Aiming at the defects of traditional four-wheel aligner such as many sensors,complex operation and slow detection speed,a fast and accurate 3D four-wheel alignment detection method is studied.Firstly,a new and special...Aiming at the defects of traditional four-wheel aligner such as many sensors,complex operation and slow detection speed,a fast and accurate 3D four-wheel alignment detection method is studied.Firstly,a new and special circle center target board is designed to calibrate the camera,and then the registration of the homography matrix is optimized by using the improved RANSAC(Random sample consensus)algorithm combined with the designed special target board,and the parameters of the wheel alignment system are adjusted by using the space vector principle.Accurate measurements are made to obtain the parameters of the four-wheel alignment.Design a calibration comparison experiment between the traditional target board and the new type of target board,and conduct a comparative test with the existing four-wheel aligner of the depot.The experimental results show that the use of the new target board-binding optimization algorithm can improve the calibration efficiency by about 9%to 21%,while improving the calibration accuracy by about 10.6%to 17.8%.And through the real vehicle test,it is verified that the use of the new target combined with the optimization algorithm can ensure the accuracy and reliability of the four-wheel positioning.This method has a certain significance in the rapid detection of vehicle four-wheel alignment parameters.展开更多
This study searches for use of simplex theory in talent management. It is a research topic belonging to this study. Human resource management （HRM） can be described with performance focus and talent management. This...This study searches for use of simplex theory in talent management. It is a research topic belonging to this study. Human resource management （HRM） can be described with performance focus and talent management. This study presents a new perspective in talent management. Firstly, Talent management may be described with fulfilling organizational positions by bets talents, because talents further performance of departments and performance of firm. Firm has departments, such as production department, marketing departments, finance department, and etc.. This study suggests simplex method for talent management for practitioners. It identifies research question and has two propositions that simplex may be used in talent management. Secondly, study depicts linear of American HRM It is based on a relationship among human resource （HR） systems, various HRM practices, and organizational performance. Linear proposition of study is that, HRM practices as a system have an impact on firm performance （goal function）.展开更多
[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize a...[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.展开更多
With further increase of the number of on-chip device, the bus structure has not met the requirements. In order to make better communication between each part, the chip designers need to explore a new structure to sol...With further increase of the number of on-chip device, the bus structure has not met the requirements. In order to make better communication between each part, the chip designers need to explore a new structure to solve the interconnection of on-chip device. The paper proposes a network-on-chip dynamic and adaptive algorithm which selects NoC platform with 2-dimension mesh as the carrier, incorporates communication energy consumption and delay into unified cost function and uses ant colony optimization to realize NOC map facing energy consumption and delay. The experiment indicates that compared with random map, single objective optimization can separately saves （30% - 47 %） and （ 20% - 39%） in communication energy consumption and execution time compared with random map, and joint objective optimization can further excavate the potential of time dimension in mapping scheme dominated by the energy.展开更多
Dynamic mutations of simple sequence repeats （SSRs） have been demonstrated to affect normal gene function and cause different genetic disorders. Several conserved and even partial functional SSR patterns are discove...Dynamic mutations of simple sequence repeats （SSRs） have been demonstrated to affect normal gene function and cause different genetic disorders. Several conserved and even partial functional SSR patterns are discovered in inherited orthologous disease genes. To explore a wide range of SSRs in genetic diseases, a comprehensive system focusing on identifying orthologous SSRs of disease genes through a comparative genomics mechanism is constructed and accomplished by adopting online Mendelian inheritance in man （OMIM） and NCBI HomoloGene databases as the fundamental resources of human genetic diseases and homologous gene information. In addition, an efficient and effective algorithm for searching SSR patterns is also developed for providing annotated SSR information among various model species. By integrating these data resources and mining technologies, biologists and doctors can systematically retrieve novel and important conserved SSR information among orthologous disease genes. The proposed system, Orthologous SSR for Disease Genes （OSDG）, is the first comprehensive framework for identifying orthologous SSRs as potential causative factors of genetic disorders and is freely available at http：//osdg.cs.ntou.edu.tw/.展开更多
In this work,we propose incorporating the finite cell method(FCM)into the absolute nodal coordinate formulation(ANCF)to improve the efficiency and robustness of ANCF elements in simulating structures with complex loca...In this work,we propose incorporating the finite cell method(FCM)into the absolute nodal coordinate formulation(ANCF)to improve the efficiency and robustness of ANCF elements in simulating structures with complex local features.In addition,an adaptive subdomain integration method based on a triangulation technique is devised to avoid excessive subdivisions,largely reducing the computational cost.Numerical examples demonstrate the effectiveness of the proposed method in large deformation,large rotation and dynamics simulation.展开更多
文摘采用高效液相色谱法,以丁烯基苯酞为内参物,建立川芎中1种酚酸类成分和5种苯酞类成分的单标多组分HPLC定量分析方法,并考察和验证该方法在川芎质量评价中应用的可行性和准确性。实验所用流动相为乙腈-0.2%甲酸水溶液,流速为1.0 m L·min-1,柱温30℃,检测波长分别为252 nm(阿魏酸、藁本内酯、丁烯基苯酞)和266 nm(洋川芎内酯I、洋川芎内酯A、阿魏酸松柏酯),进样量20μL,梯度洗脱。结果显示,单标多组分HPLC定量分析法计算所得值与外标法实测值之间无显著性差异,RSD值均小于5%;相对校正因子的耐用性较好,色谱条件改变后,相对校正因子变化的RSD值均小于5%。所建立的单标多组分HPLC定量分析法可准确地用于中药川芎的多指标同步质量控制。
文摘To gain superior computational efficiency, it might be necessary to change the underlying philosophy of the simplex method. In this paper, we propose a Phase-1 method along this line. We relax not only the conventional condition that some function value increases monotonically, but also the condition that all feasible variables remain feasible after basis change in Phase-1. That is, taking a purely combinatorial approach to achieving feasibility. This enables us to get rid of ratio test in pivoting, reducing computational cost per iteration to a large extent. Numerical results on a group of problems are encouraging.
基金Anhui Province Key Research and Development Program(No.2022107020012)Shenzhen Science and Technology Innovation Project(No.JSGG20191129102008260)。
文摘Aiming at the defects of traditional four-wheel aligner such as many sensors,complex operation and slow detection speed,a fast and accurate 3D four-wheel alignment detection method is studied.Firstly,a new and special circle center target board is designed to calibrate the camera,and then the registration of the homography matrix is optimized by using the improved RANSAC(Random sample consensus)algorithm combined with the designed special target board,and the parameters of the wheel alignment system are adjusted by using the space vector principle.Accurate measurements are made to obtain the parameters of the four-wheel alignment.Design a calibration comparison experiment between the traditional target board and the new type of target board,and conduct a comparative test with the existing four-wheel aligner of the depot.The experimental results show that the use of the new target board-binding optimization algorithm can improve the calibration efficiency by about 9%to 21%,while improving the calibration accuracy by about 10.6%to 17.8%.And through the real vehicle test,it is verified that the use of the new target combined with the optimization algorithm can ensure the accuracy and reliability of the four-wheel positioning.This method has a certain significance in the rapid detection of vehicle four-wheel alignment parameters.
文摘This study searches for use of simplex theory in talent management. It is a research topic belonging to this study. Human resource management （HRM） can be described with performance focus and talent management. This study presents a new perspective in talent management. Firstly, Talent management may be described with fulfilling organizational positions by bets talents, because talents further performance of departments and performance of firm. Firm has departments, such as production department, marketing departments, finance department, and etc.. This study suggests simplex method for talent management for practitioners. It identifies research question and has two propositions that simplex may be used in talent management. Secondly, study depicts linear of American HRM It is based on a relationship among human resource （HR） systems, various HRM practices, and organizational performance. Linear proposition of study is that, HRM practices as a system have an impact on firm performance （goal function）.
基金Supported by Major Projects for Cultivation of New Varieties ofGenetically Modified Organisms (2009ZX08010-011B)
文摘[Objective]The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method]Using inbred line "Chang 7-2" and hybrid "Zhengdan 958" of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for10 d were investigated.[Result]The rate of albino seedlings not only was related to kanamycin concentration,but also had relationship with solution volume during soaking process.The difference between inbred line and hybrid was no significant.When 100 ml of kanamycin solution with concentration of 200 mg/L was used to soak seeds for 3 d,the rate of albino seedlings basically could reach 100%.When 100 ml of kanamycin solution with concentration of 100 mg/L was used to soak 20 seeds for 3 d to carry out resistance screening,the accuracy was up to 84.8% after verifying the screening test of T1 transgenic maize plants.[Conclusion]The method was feasible,which could be used as a simple method for screening transgenic gene maize with kanamycin resistance.
文摘With further increase of the number of on-chip device, the bus structure has not met the requirements. In order to make better communication between each part, the chip designers need to explore a new structure to solve the interconnection of on-chip device. The paper proposes a network-on-chip dynamic and adaptive algorithm which selects NoC platform with 2-dimension mesh as the carrier, incorporates communication energy consumption and delay into unified cost function and uses ant colony optimization to realize NOC map facing energy consumption and delay. The experiment indicates that compared with random map, single objective optimization can separately saves （30% - 47 %） and （ 20% - 39%） in communication energy consumption and execution time compared with random map, and joint objective optimization can further excavate the potential of time dimension in mapping scheme dominated by the energy.
文摘Dynamic mutations of simple sequence repeats （SSRs） have been demonstrated to affect normal gene function and cause different genetic disorders. Several conserved and even partial functional SSR patterns are discovered in inherited orthologous disease genes. To explore a wide range of SSRs in genetic diseases, a comprehensive system focusing on identifying orthologous SSRs of disease genes through a comparative genomics mechanism is constructed and accomplished by adopting online Mendelian inheritance in man （OMIM） and NCBI HomoloGene databases as the fundamental resources of human genetic diseases and homologous gene information. In addition, an efficient and effective algorithm for searching SSR patterns is also developed for providing annotated SSR information among various model species. By integrating these data resources and mining technologies, biologists and doctors can systematically retrieve novel and important conserved SSR information among orthologous disease genes. The proposed system, Orthologous SSR for Disease Genes （OSDG）, is the first comprehensive framework for identifying orthologous SSRs as potential causative factors of genetic disorders and is freely available at http：//osdg.cs.ntou.edu.tw/.
基金supported by the National Natural Science Foundation of China(Grant Nos.52175223,and 11802072)the Fundamental Research Funds for the Central Universities(Grant No.B210201038).
文摘In this work,we propose incorporating the finite cell method(FCM)into the absolute nodal coordinate formulation(ANCF)to improve the efficiency and robustness of ANCF elements in simulating structures with complex local features.In addition,an adaptive subdomain integration method based on a triangulation technique is devised to avoid excessive subdivisions,largely reducing the computational cost.Numerical examples demonstrate the effectiveness of the proposed method in large deformation,large rotation and dynamics simulation.
