为了分析樱桃番茄‘VFNT Cherry’(syn.LA1221)和加工番茄‘Heinz1706’组配的F1代果实质量超亲杂种优势的遗传基础,利用其F2群体,通过QTL-seq和单标记分析法进行了果实质量QTL定位,共检测到10个果实质量位点,其中8个位点的大果等位基...为了分析樱桃番茄‘VFNT Cherry’(syn.LA1221)和加工番茄‘Heinz1706’组配的F1代果实质量超亲杂种优势的遗传基础,利用其F2群体,通过QTL-seq和单标记分析法进行了果实质量QTL定位,共检测到10个果实质量位点,其中8个位点的大果等位基因为显性,包括部分显性位点5个[QTL for Fruit Weight 2.1(qFW2.1)、qFW5.2、qFW7.1、qFW8.1、qFW9.1]和超显性位点3个(qFW1.1、qFW5.1、qFW6.1)。而且qFW2.1、qFW5.1、qFW7.1、qFW8.1、qFW9.1的大果等位基因来自‘Heinz1706’,qFW1.1、qFW5.2、qFW6.1的大果等位基因来自‘VFNTCherry’。因此F1代番茄果实质量出现超亲现象可能是由于其聚合了来自双亲的显性大果等位基因。上述8个显性位点中qFW9.1的表型变异贡献率最高,达到12.19%。通过交换单株后代鉴定进一步验证了qFW9.1位点并将其定位区间缩小到4.5 Mb。展开更多
Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable condit...Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable conditions.To investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840,a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse.A major quantitative trait locus(QTL),derived from Sumai 3,was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis(LTN).The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai 3.Unlike Lr34,lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size,and a smaller effect on severity.Additive effects were observed between lm and Lr34 for severity and LTN,and an epistatic effect was observed for infection type.Single marker analysis also identified several other QTL with minor effects on severity,infection type,or LTN.展开更多
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat （SSR） genotyping data. The amplified fragments from SSR primers were highly polymorphic in the bad...The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat （SSR） genotyping data. The amplified fragments from SSR primers were highly polymorphic in the badey accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 badey accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.展开更多
文摘为了分析樱桃番茄‘VFNT Cherry’(syn.LA1221)和加工番茄‘Heinz1706’组配的F1代果实质量超亲杂种优势的遗传基础,利用其F2群体,通过QTL-seq和单标记分析法进行了果实质量QTL定位,共检测到10个果实质量位点,其中8个位点的大果等位基因为显性,包括部分显性位点5个[QTL for Fruit Weight 2.1(qFW2.1)、qFW5.2、qFW7.1、qFW8.1、qFW9.1]和超显性位点3个(qFW1.1、qFW5.1、qFW6.1)。而且qFW2.1、qFW5.1、qFW7.1、qFW8.1、qFW9.1的大果等位基因来自‘Heinz1706’,qFW1.1、qFW5.2、qFW6.1的大果等位基因来自‘VFNTCherry’。因此F1代番茄果实质量出现超亲现象可能是由于其聚合了来自双亲的显性大果等位基因。上述8个显性位点中qFW9.1的表型变异贡献率最高,达到12.19%。通过交换单株后代鉴定进一步验证了qFW9.1位点并将其定位区间缩小到4.5 Mb。
基金partly funded by the Priority Academic Program Development of Jiangsu Higher Education Institutionthe National Research Initiative of the USDA Cooperative State Research,Education and Extension Service,CAP (2006-55606-16629)the Kan-sas Agricultural Experiment Station,Manhattan,Kansas,USA (10-325-J)
文摘Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable conditions.To investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840,a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse.A major quantitative trait locus(QTL),derived from Sumai 3,was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis(LTN).The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai 3.Unlike Lr34,lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size,and a smaller effect on severity.Additive effects were observed between lm and Lr34 for severity and LTN,and an epistatic effect was observed for infection type.Single marker analysis also identified several other QTL with minor effects on severity,infection type,or LTN.
基金Project supported by the National Natural Science Foundation of China (Nos. 30700485 and 30771333)the Zhejiang Provincial Natural Science Foundation (No. Y306641),China
文摘The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat （SSR） genotyping data. The amplified fragments from SSR primers were highly polymorphic in the badey accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 badey accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.
