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小麦TaMAPK1基因的克隆及其功能研究
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作者 张潇予 吴保为 +3 位作者 张思雨 马猛 刘香利 赵惠贤 《麦类作物学报》 CAS CSCD 北大核心 2023年第5期537-544,共8页
丝裂原活化蛋白激酶(MAPK)在植物生长发育与逆境应答过程中具有重要作用。为探究小麦MAPK基因在植物生长发育中的作用,利用生物信息学分析其编码蛋白的序列和结构,发现该基因编码的蛋白含有一个保守的催化丝氨酸/苏氨酸磷酸化的激酶结构... 丝裂原活化蛋白激酶(MAPK)在植物生长发育与逆境应答过程中具有重要作用。为探究小麦MAPK基因在植物生长发育中的作用,利用生物信息学分析其编码蛋白的序列和结构,发现该基因编码的蛋白含有一个保守的催化丝氨酸/苏氨酸磷酸化的激酶结构域,属于丝氨酸/苏氨酸蛋白激酶。系统进化分析结果表明,小麦MAPK基因与拟南芥MAPK1为直系同源基因,故将其命名为TaMAPK1。进一步通过转基因方法创建了以野生型拟南芥Col-0为背景的TaMAPK1过表达株系(TaMAPK1-OE)和以拟南芥AtMAPK1基因功能缺失纯合突变体mapk1为遗传背景的TaMAPK1回补系(TaMAPK1-com),并对其进行表型观察和产量性状调查,结果发现,突变体mapk1的抽薹时间比Col-0提前2.8 d,TaMAPK1-com的抽薹时间与Col-0无显著变化;与Col-0相比,突变体mapk1的株高、总分枝数、种子大小、单株种子重量和单株生物量均显著提高,而TaMAPK1-com和TaMAPK1-OE株系的种子大小、单株和种子重量和单株生物量均显著减少。 展开更多
关键词 小麦 TaMAPK1 抽薹时期 种子大小 单株种子重量
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Response of Peach (Prunus persica) cv. to Foliar Application of Potassium and Copper
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作者 Shawkat Mustafa Mohammed AI-Atrushy Sarfaraz Fatah Ali Al-bamarny 《Journal of Agricultural Science and Technology(A)》 2013年第1期47-52,共6页
The experiment was carried out in a private orchard situated at Atrush town, Duhok Govemorate Kurdistan Region-Iraq during 2011 season to study the effect of foliar application of three concentrations of potassium (0... The experiment was carried out in a private orchard situated at Atrush town, Duhok Govemorate Kurdistan Region-Iraq during 2011 season to study the effect of foliar application of three concentrations of potassium (0.0%, 0.5% and 1%) and three concentrations of copper (0.0%, 0.02% and 0.04%) on an eight years old peach cultivar, planted in clay soil, spaced at 4.0 m x 4.0 m. The foliar application was done twice, on April 24, 2011 and May 25, 2011. The results showed that spraying of potassium at both concentration (0.5% and 1%) or copper at high concentration (0.04%) had a positive effect on leaf area, leaf fresh weight, leaf dry weight, total chlorophyll, fruit weight, fruit number and yield per tree as well as fruit diameter, pith thick, pulp weight, seed weight and total soluble solid. The interaction between potassium and copper significantly enhanced all detected traits, since trees receiving 1% and sprayed with 0.04% of copper was characterized by the highest values of all growth and yield characteristics compared to the lowest values at untreated trees (control). 展开更多
关键词 POTASSIUM COPPER PEACH Amish.
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