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灯盏菊(Erigeron breviscapus (vant.) Hand-mazz)单株组培工厂化生产种苗技术研究 被引量:2
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作者 马玉芳 许继宏 梅慧 《云南大学学报(自然科学版)》 CAS CSCD 2001年第S1期113-116,共4页
筛选了药用植物灯盏菊愈伤组织诱导、增殖及生根培养基配方 ,首次用单一灯盏菊植株外植体进行诱导产生的愈伤组织转移诱导出灯盏菊芽 .完成了该种植物组培苗生根和入土栽培的整套技术研究 ,为灯盏菊的人工栽培和育种提供了一个新的途径 .
关键词 灯盏菊 单株组培 快繁技术
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Research on Tissue Culture and Rapid Propagation Technology of Superior Individuals of Lonicera edulis Turcz 被引量:5
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作者 金灿 曹后男 +2 位作者 宗成文 李文剑 安丰云 《Agricultural Science & Technology》 CAS 2011年第11期1585-1588,共4页
[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as m... [Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS+1.0 mg/L of 6-BA + 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS+ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite (1∶ 1, V/V), survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production. 展开更多
关键词 Lonicera edulis Turcz Tissue culture Superior individual
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