目的:评价局部骨髓单核细胞移植对大鼠缺血心肌血管生成作用及对血流动力学的影响。方法:将Lwe is大鼠26只随机分为移植组(n=14)及对照组(n=12),结扎大鼠左冠脉管降支,建立急性心肌梗死模型。将新分离的骨髓单核细胞注射到大鼠的缺血心...目的:评价局部骨髓单核细胞移植对大鼠缺血心肌血管生成作用及对血流动力学的影响。方法:将Lwe is大鼠26只随机分为移植组(n=14)及对照组(n=12),结扎大鼠左冠脉管降支,建立急性心肌梗死模型。将新分离的骨髓单核细胞注射到大鼠的缺血心肌中,术后4周观察心肌血管数目,结合心脏血流动力学参数评价心功能的改善情况。结果:4周后血流动力学检测移植组相比对照组左心室舒张末期压力降低,有统计学意义(P<0.01);压力变化率最大值(dp/dt)升高,有统计学意义(P<0.01)。骨髓单核细胞心肌内移植的大鼠中,毛细血管密度大大增加,移植组和对照组心肌血管数分别为23.1±1.5个/HPF vs 10.5±1.8个/HPF,P<0.01。结论:局部移植骨髓单核细胞能明显促进缺血心肌新生血管生成,增加缺血区灌注,并可在一定程度上改善血流动力学。展开更多
AIM: To study the inhibitory effect of mononuclear bone marrow cell (BMC) transplantation on carbon tetrachloride (CCl4) -induced liver fibrosis in rats.METHODS: Rat liver fibrosis models were induced by CCl4 and alco...AIM: To study the inhibitory effect of mononuclear bone marrow cell (BMC) transplantation on carbon tetrachloride (CCl4) -induced liver fibrosis in rats.METHODS: Rat liver fibrosis models were induced by CCl4 and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group (n = 10) and control group (n = 10). BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl4 was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Liver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis (hydroxyproline and collagen fibre) and liver pathological grades in rats were evaluated. RESULTS: The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6 ± 128.8 mg/g in treatment group, and 596.0 ± 341.8 mg/g in control group. The percentage of collagen fibre was 3.75% ± 0.98% in treatment group and 5.02% ± 0.44% in control group. There was a significant difference between the two groups (P < 0.05). Liver pathological grade decreased from grade Ⅳ to grade Ⅲ partially in treatment group (P < 0.05) with no obvious improvement in control group (P > 0.05). There was a significant difference between treatment group and control group (P < 0.05).CONCLUSION: Transplantation of BMC can improve liver fibrosis due to chronic liver injury in rats.展开更多
文摘目的:评价局部骨髓单核细胞移植对大鼠缺血心肌血管生成作用及对血流动力学的影响。方法:将Lwe is大鼠26只随机分为移植组(n=14)及对照组(n=12),结扎大鼠左冠脉管降支,建立急性心肌梗死模型。将新分离的骨髓单核细胞注射到大鼠的缺血心肌中,术后4周观察心肌血管数目,结合心脏血流动力学参数评价心功能的改善情况。结果:4周后血流动力学检测移植组相比对照组左心室舒张末期压力降低,有统计学意义(P<0.01);压力变化率最大值(dp/dt)升高,有统计学意义(P<0.01)。骨髓单核细胞心肌内移植的大鼠中,毛细血管密度大大增加,移植组和对照组心肌血管数分别为23.1±1.5个/HPF vs 10.5±1.8个/HPF,P<0.01。结论:局部移植骨髓单核细胞能明显促进缺血心肌新生血管生成,增加缺血区灌注,并可在一定程度上改善血流动力学。
基金the Key Scientific and Technological Projects of Guangdong Province, No. 2005A30201007Guangdong Provincial Natural Science Fundation, No. 06021343
文摘AIM: To study the inhibitory effect of mononuclear bone marrow cell (BMC) transplantation on carbon tetrachloride (CCl4) -induced liver fibrosis in rats.METHODS: Rat liver fibrosis models were induced by CCl4 and alcohol administration. After 8 wk, twenty rats were randomly allocated into treatment group (n = 10) and control group (n = 10). BMC were infused into the rats in treatment group via the portal vein, while heparinized saline was infused in control group. CCl4 was hypodermically injected into the rats twice a week for 4 wk. At the end of wk 12, all rats were humanely sacrificed. Liver samples were taken and stained with HE or Masson trichrome. The general conditions, liver fibrosis (hydroxyproline and collagen fibre) and liver pathological grades in rats were evaluated. RESULTS: The general conditions of the rats in treatment group improved markedly, but not in control group. Hydroxyproline was 504.6 ± 128.8 mg/g in treatment group, and 596.0 ± 341.8 mg/g in control group. The percentage of collagen fibre was 3.75% ± 0.98% in treatment group and 5.02% ± 0.44% in control group. There was a significant difference between the two groups (P < 0.05). Liver pathological grade decreased from grade Ⅳ to grade Ⅲ partially in treatment group (P < 0.05) with no obvious improvement in control group (P > 0.05). There was a significant difference between treatment group and control group (P < 0.05).CONCLUSION: Transplantation of BMC can improve liver fibrosis due to chronic liver injury in rats.
