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基于多源域适应的单细胞智能分类
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作者 魏琢艺 罗迈 +3 位作者 李文兵 曾远松 余伟江 杨跃东 《计算机工程》 CAS CSCD 北大核心 2024年第6期48-55,共8页
单细胞核糖核酸(RNA)测序技术被成功应用于产生人体组织和器官的高分辨率细胞图谱,这加深了研究者们对人类疾病组织中细胞异质性的理解。细胞注释是单细胞RNA测序数据分析中非常关键的一步,许多典型的模型利用一个有标签的单细胞参考数... 单细胞核糖核酸(RNA)测序技术被成功应用于产生人体组织和器官的高分辨率细胞图谱,这加深了研究者们对人类疾病组织中细胞异质性的理解。细胞注释是单细胞RNA测序数据分析中非常关键的一步,许多典型的模型利用一个有标签的单细胞参考数据集去注释目标数据集,但目标数据集中部分细胞类型可能不在参考数据集中。整合多个参考数据集可以更好地覆盖目标数据集中的细胞类型,然而多个参考数据集和目标数据集之间存在因测序技术差异等原因造成的批次效应。为此,提出一种基于多源域适应的单细胞分类模型,利用多个已标注细胞类型的参考数据集分别与未标注细胞类型的目标数据集进行对抗训练,消除了批次效应。采用虚拟对抗训练,进一步提升模型预测结果对数据点周围局部微小扰动或噪声的鲁棒性,防止过拟合。在多个单细胞数据集上的实验结果表明,该模型比目前主流模型的细胞识别精度至少提升了5个百分点,为新测序的单细胞身份鉴定提供了新的选择和参考。 展开更多
关键词 单细胞核糖核酸测序 单细胞分类 多源域适应 对抗训练 深度学习
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隐私保护下单细胞RNA测序数据细胞分类研究
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作者 徐文嘉 岑孟杰 陈亮 《医学信息学杂志》 CAS 2024年第10期86-89,共4页
目的/意义研究安全的高维稀疏数据处理方法,提高分析精度并保障敏感信息安全,促进单细胞RNA测序技术的广泛应用。方法/过程提出基于可信执行环境(trusted execution environment,TEE)的解决方案,将训练数据加密后传输至TEE,在安全隔离... 目的/意义研究安全的高维稀疏数据处理方法,提高分析精度并保障敏感信息安全,促进单细胞RNA测序技术的广泛应用。方法/过程提出基于可信执行环境(trusted execution environment,TEE)的解决方案,将训练数据加密后传输至TEE,在安全隔离环境中解密并训练,获得训练后的模型参数。对比分析TEE和传统明文环境下使用基于神经网络的自动细胞类型识别模型和支持向量机进行细胞分类的表现。结果/结论TEE下两种分类模型的F 1分别达到0.904和0.879,与传统明文环境下性能相当;TEE提供的安全执行环境对模型的准确性和效率影响极有限,可用于处理敏感或私有数据场景。 展开更多
关键词 可信执行环境 单细胞RNA测序 单细胞分类 隐私保护
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Predicting the Reproduction Strategies of Several Microalgae Through Their Genome Sequences 被引量:1
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作者 GUO Li YANG Guanpin 《Journal of Ocean University of China》 SCIE CAS 2015年第3期491-502,共12页
Documenting the sex and sexual reproduction of the microalgae is very difficult, as most of the results are based on the microscopic observation that can be heavily influenced by genetic, physiological and environment... Documenting the sex and sexual reproduction of the microalgae is very difficult, as most of the results are based on the microscopic observation that can be heavily influenced by genetic, physiological and environmental conditions. Understanding the reproduction strategy of some microalgae is required to breed them in large scale culture industry. Instead of direct observation of sex and sexual reproduction under microscope, the whole set or the majority of core meiosis genes may evidence the sex and sexual reproduction in the unicellular algae, as the meiosis is necessary for maintaining the genomic stability and the advantages of genetic recombination. So far, the available genome sequences and bioinformatic tools (in this study, homolog searching and phylogenetic analysis) allow us to propose that at least 20 core meiosis genes (among them 〉6 must be meiosis specific) are enough for an alga to maintain its sexual reproduction. According to this assumption and the genome sequences, it is possible that sexual reproduction was carried out by Micromonas pusilla and Cyanidiosehyzon merolae, while asexual reproduction was adopted by Bigelowiella natans, Guillardia theta, Nannochloropsis gaditana, N. oeeanica, Chlorella variablis, Phaeodactylum tricornutum and Thalassiosira pseu- donana. This understanding will facilitate the breeding trials of some economic microalgae (e.g., N. gaditana, N. oceanica, C. vari- ablis and P. tricornutum). However, the reproduction strategies of these microalgae need to be proved by further biological experiments. 展开更多
关键词 MICROALGA sexual reproduction MEIOSIS core meiosis gene meiosis specific gene homolog searching phylogenetic analysis
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THE GLOBAL STABILITY OF A COMPETING CHEMOSTAT MODEL WITH DELAYED NUTRIENT RECYCLING 被引量:1
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作者 JIANGLiqiang MAZhien P.Fergola 《Journal of Systems Science & Complexity》 SCIE EI CSCD 2005年第1期19-26,共8页
In this paper, by applying the method of Liapunov functionals we study the global stability of the positive equilibrium of a competing chemostat model with delayed nutrient recycling. The sufficient conditions of the ... In this paper, by applying the method of Liapunov functionals we study the global stability of the positive equilibrium of a competing chemostat model with delayed nutrient recycling. The sufficient conditions of the global stability (involved in average time delay or not) are obtained. 展开更多
关键词 CHEMOSTAT distributed time delay positive equilibrium global stability
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