目的运用荧光原位杂交技术(fluorescent in situ hybridization,FISH)对废弃胚胎固定好的单卵裂球进行检测,了解染色体数目异常,即非整倍体(anenploidy)改变在体外受精(in vitro-fertilization,IVF)中的发生情况。方法应用橘红色LSI-21...目的运用荧光原位杂交技术(fluorescent in situ hybridization,FISH)对废弃胚胎固定好的单卵裂球进行检测,了解染色体数目异常,即非整倍体(anenploidy)改变在体外受精(in vitro-fertilization,IVF)中的发生情况。方法应用橘红色LSI-21探针对IVF患者正常受精D3的废弃胚胎活检后单个卵裂球进行FISH检测,统计并分析其信号结果。结果活检胚胎33个,2个胚胎未出现信号,获31个胚胎,共64个卵裂球杂交信号。包括年龄≥35岁患者胚胎7个,<35岁患者胚胎24个。其中2倍体信号胚胎20个,单倍体信号胚胎3个,3倍体信号胚胎8个,21号染色体异常的胚胎为11个,其中35岁以上患者占3个,35岁以下患者占8个。结论人IVF废弃胚胎21号染色体异常几率多。患者年龄≥35岁者尤甚。展开更多
Biodesulfurization （BDS） is a promising technology for deep desulfurization. In this work, Pseudomonas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels. It is ...Biodesulfurization （BDS） is a promising technology for deep desulfurization. In this work, Pseudomonas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels. It is found that thiophene and dibenzothiophene （DBT） can be simultaneously metabolized by immobilized R-8 cells. The initial sulfur content in the model oil is 300 mg·kg-1 （thiophene ＂ DI3T= 1 ＂ 1）. After 10 h of treatment, the thiophene concentration is reduced by 40%, while DBT is reduced by 25%. The utilization rate of thiophene is faster than that of DBT. Moreover, the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems. Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5 ： 1. The immobilized cells are successfully reused over 15 batch cycles. In the last batch, the desulfurization activity remains at least 75% of the first batch.展开更多
文摘目的运用荧光原位杂交技术(fluorescent in situ hybridization,FISH)对废弃胚胎固定好的单卵裂球进行检测,了解染色体数目异常,即非整倍体(anenploidy)改变在体外受精(in vitro-fertilization,IVF)中的发生情况。方法应用橘红色LSI-21探针对IVF患者正常受精D3的废弃胚胎活检后单个卵裂球进行FISH检测,统计并分析其信号结果。结果活检胚胎33个,2个胚胎未出现信号,获31个胚胎,共64个卵裂球杂交信号。包括年龄≥35岁患者胚胎7个,<35岁患者胚胎24个。其中2倍体信号胚胎20个,单倍体信号胚胎3个,3倍体信号胚胎8个,21号染色体异常的胚胎为11个,其中35岁以上患者占3个,35岁以下患者占8个。结论人IVF废弃胚胎21号染色体异常几率多。患者年龄≥35岁者尤甚。
基金Supported by the National Natural Science Foundation of China (21076216,30970046)
文摘Biodesulfurization （BDS） is a promising technology for deep desulfurization. In this work, Pseudomonas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels. It is found that thiophene and dibenzothiophene （DBT） can be simultaneously metabolized by immobilized R-8 cells. The initial sulfur content in the model oil is 300 mg·kg-1 （thiophene ＂ DI3T= 1 ＂ 1）. After 10 h of treatment, the thiophene concentration is reduced by 40%, while DBT is reduced by 25%. The utilization rate of thiophene is faster than that of DBT. Moreover, the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems. Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5 ： 1. The immobilized cells are successfully reused over 15 batch cycles. In the last batch, the desulfurization activity remains at least 75% of the first batch.
