扫描离子电导显微镜(scanning ion conductance microscopy,SICM)是一种非接触式的扫描探针显微技术(scanning probe microscopy,SPM),可以实现生物样品在近生理条件下的成像。随着技术发展,目前广泛应用于生物医学领域的SICM主要包括两...扫描离子电导显微镜(scanning ion conductance microscopy,SICM)是一种非接触式的扫描探针显微技术(scanning probe microscopy,SPM),可以实现生物样品在近生理条件下的成像。随着技术发展,目前广泛应用于生物医学领域的SICM主要包括两种:跳跃式离子电导显微技术(hopping probe ion conductance microscopy,HPICM)和外加压力模式的SICM。前者可以应用于软的、黏的、对外力或其它机械信号敏感的样品的高分辨成像;后者可以通过探针微管对样品局部施加外力刺激或化学、电学、光学或生物分子等信号,实现对样品动力学性质或相关生理过程局部的原位研究。此外,SICM技术具有良好的开放性,能够越来越多地与其它技术手段联用,极大地丰富了其在生物医学领域的应用,可用于疾病发病机理、药物作用以及临床诊断等的研究。但是,目前SICM时间分辨率较低,这制约了它在生物体系动力学行为方面的研究。展开更多
Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and dynamics.With the rapid merging of biotechnology and nanotechnology in recent yea...Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and dynamics.With the rapid merging of biotechnology and nanotechnology in recent years,single plasmonic nanoparticle sensors have endowed a new dimension to the imaging scale given their comparable size to biomolecules such as nucleic acids or antibodies.展开更多
Single-particle tracking photoactivated local- ization microscopy （sptPALM） has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we hav...Single-particle tracking photoactivated local- ization microscopy （sptPALM） has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-β type II receptor （TβRII） at high expression level using both traditional single-particle tracking （SPT） and sptPALM. The ligand-induced aggregation of TβRII oligomers was further indicated by sptPALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, sptPALM outperforms traditional SPT by providing more accurate biophysical information,展开更多
文摘扫描离子电导显微镜(scanning ion conductance microscopy,SICM)是一种非接触式的扫描探针显微技术(scanning probe microscopy,SPM),可以实现生物样品在近生理条件下的成像。随着技术发展,目前广泛应用于生物医学领域的SICM主要包括两种:跳跃式离子电导显微技术(hopping probe ion conductance microscopy,HPICM)和外加压力模式的SICM。前者可以应用于软的、黏的、对外力或其它机械信号敏感的样品的高分辨成像;后者可以通过探针微管对样品局部施加外力刺激或化学、电学、光学或生物分子等信号,实现对样品动力学性质或相关生理过程局部的原位研究。此外,SICM技术具有良好的开放性,能够越来越多地与其它技术手段联用,极大地丰富了其在生物医学领域的应用,可用于疾病发病机理、药物作用以及临床诊断等的研究。但是,目前SICM时间分辨率较低,这制约了它在生物体系动力学行为方面的研究。
文摘Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and dynamics.With the rapid merging of biotechnology and nanotechnology in recent years,single plasmonic nanoparticle sensors have endowed a new dimension to the imaging scale given their comparable size to biomolecules such as nucleic acids or antibodies.
基金supported by the National Basic Research Program of China(2013CB933701)the National Natural Science Foundation of China(21127901+2 种基金9141311991213305)the Chinese Academy of Science
文摘Single-particle tracking photoactivated local- ization microscopy （sptPALM） has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-β type II receptor （TβRII） at high expression level using both traditional single-particle tracking （SPT） and sptPALM. The ligand-induced aggregation of TβRII oligomers was further indicated by sptPALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, sptPALM outperforms traditional SPT by providing more accurate biophysical information,
