以盆栽的C4植物-湖南稷子 Echinochloafrumentacea 为材料,用6-苄氨基嘌呤 BA 和脱落酸 ABA 定位涂抹湖南稷子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节.实验结果表明,ABA和具有...以盆栽的C4植物-湖南稷子 Echinochloafrumentacea 为材料,用6-苄氨基嘌呤 BA 和脱落酸 ABA 定位涂抹湖南稷子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节.实验结果表明,ABA和具有细胞分裂素活性的BA是调控Na+、K+及游离脯氨酸在不同层位叶中分配的重要因素.ABA涂抹湖南稷子的上位叶片,上位叶片中的Na+比其下位叶片高35.0%;用ABA涂抹湖南稷子的下位叶片,下位叶片中的K+比其上位叶片高31.4%,下位叶鞘中的K+比其上位叶鞘高53.7%.用BA涂抹湖南稷子的下位叶片,下位叶片中的K+和脯氨酸分别比其上位叶片高16.5%和31.7%;用BA或ABA定位涂抹植物地上不同部位,引起植物整株水平上Na+、K+向光合作用强的部位,特别是向活跃期的穗中选择性运输的能力增强,游离脯氨酸也多集中于代谢旺盛的光合器官和生殖器官.展开更多
Cyclodextrins are widely used for various purposes and limited because of it′s low solubility.Branched cyclodextrins have attracted more and more attention because of their many advantages such as higher solubility i...Cyclodextrins are widely used for various purposes and limited because of it′s low solubility.Branched cyclodextrins have attracted more and more attention because of their many advantages such as higher solubility in water.In this study a new method for synthesizing maltosyl(α-1→6)β-cyclodextrin with a high yield was described.Mal-β-CD was synthesized through the reverse reaction of Bacillus licheniformis pullulanase and identified.The factors which affect the formation of maltosyl(α1→6)β-cyclodextrin were studied and the optimum conditions were obtained as follows: 250 U/g β-CD of enzyme amount,molar ratio of maltose to β-CD 12∶1～16∶1,a substrate mass fraction of 80%,the pH of reaction mixture 4.5,reaction temperature 70 ℃ and reaction time 60 h respectively.The product was separated by Sephadex G25 column and further purified by Bio-gel P-2 column.Maltosyl(α-1→6)β-cyclodextrin was identified by IR,ESI-MS and NMR.It was proved to be a effective method to synthesize maltosyl(α-1→6)β-cyclodextrin by using Bacillus licheniformis pullulanase.The yield of Mal-β-CD was about 56% under the optimal conditions.展开更多
文摘以盆栽的C4植物-湖南稷子 Echinochloafrumentacea 为材料,用6-苄氨基嘌呤 BA 和脱落酸 ABA 定位涂抹湖南稷子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节.实验结果表明,ABA和具有细胞分裂素活性的BA是调控Na+、K+及游离脯氨酸在不同层位叶中分配的重要因素.ABA涂抹湖南稷子的上位叶片,上位叶片中的Na+比其下位叶片高35.0%;用ABA涂抹湖南稷子的下位叶片,下位叶片中的K+比其上位叶片高31.4%,下位叶鞘中的K+比其上位叶鞘高53.7%.用BA涂抹湖南稷子的下位叶片,下位叶片中的K+和脯氨酸分别比其上位叶片高16.5%和31.7%;用BA或ABA定位涂抹植物地上不同部位,引起植物整株水平上Na+、K+向光合作用强的部位,特别是向活跃期的穗中选择性运输的能力增强,游离脯氨酸也多集中于代谢旺盛的光合器官和生殖器官.
文摘Cyclodextrins are widely used for various purposes and limited because of it′s low solubility.Branched cyclodextrins have attracted more and more attention because of their many advantages such as higher solubility in water.In this study a new method for synthesizing maltosyl(α-1→6)β-cyclodextrin with a high yield was described.Mal-β-CD was synthesized through the reverse reaction of Bacillus licheniformis pullulanase and identified.The factors which affect the formation of maltosyl(α1→6)β-cyclodextrin were studied and the optimum conditions were obtained as follows: 250 U/g β-CD of enzyme amount,molar ratio of maltose to β-CD 12∶1～16∶1,a substrate mass fraction of 80%,the pH of reaction mixture 4.5,reaction temperature 70 ℃ and reaction time 60 h respectively.The product was separated by Sephadex G25 column and further purified by Bio-gel P-2 column.Maltosyl(α-1→6)β-cyclodextrin was identified by IR,ESI-MS and NMR.It was proved to be a effective method to synthesize maltosyl(α-1→6)β-cyclodextrin by using Bacillus licheniformis pullulanase.The yield of Mal-β-CD was about 56% under the optimal conditions.
