南华松叶蜂病毒DnNPV的发现及其毒力测试

One virus of Diprion nanhuaensis was separated from the died D. nanhuaensis, the virus belongs to nuclear polyhedrosis virus, bacilliform virus particle in inclusion body appears SNPV(single nucleocapsid polyhedrosis virus) by electron microscopy. The structure of inclusion body appears polyhedron and close to rotundity, and the length of diameter of inclusion body ranges 0.5～1.0 μm, a little proportion of them shows anomalistic form. There are many bacilliform virus particles in one inclusion body. Six virus particles in one inclusion body take up a large proportion and one virus particle in one inclusion body only takes up a small proportion, the length of virus particle ranges 125～200 nm,and the length of diameter of virus particle ranges 25～30 nm. Meanwhile,the virus can infect the 2～3 instars Neodiprion xiangyunicus and the 2～3 instars D. nanhuaensis in laboratory.

南华松叶蜂的发生与防治

南华松叶蜂(Diprion nanhuaensis Xiao)属膜翅目、叶蜂总科、松叶蜂科。主要是以幼虫取食针叶,一头幼虫一生可取食针叶700 cm左右,危害云南松、华山松、马尾松。通过10多年的观察与防治试验研究,基本摸清了南华松叶蜂的生活史、生物学特性、发生规律及综合防治技术,为南华松叶蜂的防治提供了科学依据。

南华松叶蜂生物学习性观察及防治试验

南华松叶蜂在贵州平坝1 a发生2代,以老熟幼虫在萤内越冬,第1代幼虫于7～8月取食2年生松针造成局部性危害,幼虫有群集习性,便于人工防治。据室内人工饲养观察,幼虫期受姬蜂及寄蝇寄生,其寄生率高居40%～88%,表明通过充分运用天敌因素,可以实现害虫的自然控制。