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不同南药多糖对鸡传染性法氏囊病毒VP2蛋白免疫原性的影响 被引量:6
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作者 朱永健 那威 +6 位作者 杨雨辉 胡日查 杨鑫 李承姮 周瑞刚 韩蒙蒙 陈云 《黑龙江畜牧兽医》 CAS 北大核心 2020年第18期119-123,共5页
为了研究不同南药多糖对鸡传染性法氏囊病毒(IBDV)VP2蛋白免疫原性的影响,试验采取水提醇沉法提取槟榔、巴戟天、砂仁、黄秋葵、牛大力、益智仁、广藿香多糖,对免疫增强剂的安全性进行评估,再将100只雏鸡随机分成10组,每组10只,分别为... 为了研究不同南药多糖对鸡传染性法氏囊病毒(IBDV)VP2蛋白免疫原性的影响,试验采取水提醇沉法提取槟榔、巴戟天、砂仁、黄秋葵、牛大力、益智仁、广藿香多糖,对免疫增强剂的安全性进行评估,再将100只雏鸡随机分成10组,每组10只,分别为益智仁组、广藿香组、牛大力组、巴戟天组、黄秋葵组、槟榔组、砂仁组、VP2蛋白组、油乳组、空白对照组,将各多糖配合鸡IBDV VP2蛋白对雏鸡进行免疫,VP2蛋白组、油乳组、空白对照组分别免疫相同剂量的VP2蛋白、油乳免疫增强剂+VP2蛋白、生理盐水。各组雏鸡于7,14,21,28日龄空腹称重并运用ELISA法测定其血清IBDV特异性抗体水平,同时对雏鸡免疫器官指数的变化进行分析。结果表明:各组雏鸡的体重、体温及进食均正常,注射部位及全身未出现异常变化。14日龄牛大力组、砂仁组雏鸡体重极显著高于油乳组(P<0.01),且显著效果持续长久,而益智仁组、巴戟天组、槟榔组雏鸡则在二免后体重极显著高于油乳组(P<0.01)。巴戟天、黄秋葵、砂仁、槟榔均能提高雏鸡的法氏囊、脾脏免疫器官指数,其中砂仁多糖与槟榔多糖的增强效果优于其他南药多糖,21,28日龄砂仁组与槟榔组的IBDV抗体水平明显高于其他组,且砂仁组在峰值后下降趋势较其他组缓慢。砂仁多糖不仅能促进雏鸡生长发育,同时与IBDV VP2蛋白具有协同作用,能有效增强脾脏与法氏囊免疫功能,提高IBDV特异性抗体水平,显著提高疫苗效率。说明南药多糖对雏鸡的成长没有不良影响,具有较好的安全性。砂仁多糖具有较高的免疫增强效果,可以应用于鸡IBDV VP2蛋白疫苗的佐剂研究。 展开更多
关键词 南药多糖 传染性法氏囊病毒 佐剂 VP2蛋白 抗体水平 促生长
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Effects of Biological Pesticides on Prevention and Treatment of Virus Diseases in Greenhouse Tomato in Autumn 被引量:1
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作者 彭昌家 白体坤 +2 位作者 丁攀 冯礼斌 杨宇衡 《Agricultural Science & Technology》 CAS 2016年第8期1921-1925,共5页
To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to c... To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to conduct field efficacy tests for Lentinan + Gibberellin-heteroauxin-brassinolide (GHB) and Ningnanmycin + GHB. The results showed that when the virus diseases in greenhouse tomato were serious, 0.5% Lentinan AS 3 000 ml + GHB WP 600 g and 8% Ningnanmycin AS 900 ml + GHB WP 600 g per hectare were applied 4 times with an interval of 7 days, and the control effects on the 10th, 17th, 26th and 35th day were above 88%, 87%, 78% and 67%, respectively. The difference in control effect of the two biological pesticides was insignificant, while their control effects were all significantly better than that of moroxydine hydrochloride (CK) + GHB. The obtained results indicated that Lentinan + GHB and Ningnanmycin + GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production. 展开更多
关键词 Tomato Virus disease Biological pesticide LENTINAN NINGNANMYCIN Gibberellin-heteroauxin-brassinolide Control effect
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